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April 21, 2023 | GenovisWebinars |

We are excited to announce the speaker and topic for our next GenovisWebinar!

LC-MS is an important technique for mAb characterization and the monitoring of potentially important attributes and modifications. Middle-level analysis is an efficient way to characterize and monitor your mAbs as it allows for much more detailed modification information to be determined compared to an intact approach. Overall efficiency of these approaches can be improved further by automating the analytical process enabling detailed middle-level characterization to be performed without the requirement for any hands-on sample preparation.
We are delighted to invite Claire Butré from Quality Assistance to describe how using FabRICATOR® HPLC columns has enabled the development of fully automated (2D-)LC-MS workflows for multi-attribute monitoring (MAM) analysis of mAbs at the subunit level in a QC-compliant manner.
Approaches like this allow for detailed mAb characterization without the need for manual sample preparation, which can make overall process optimization both faster and easier!
Title: Multiple-Attribute Monitoring in QC: Alternatives to the Bottom-up Approach
Date: April 27th, 2023
Time: 4 pm CET

Key Learning Objectives

  • Use of FabRICATOR for multiple attribute monitoring of monoclonal antibodies
  • Implementation of an automated 2D LC-MS workflow for characterization of biotherapeutics
  • How to set up on-line reduction

Who Should Attend?

  • Scientists involved in the MS characterization of biopharmaceuticals
  • Scientists performing middle-level analysis of mAbs
  • Scientists with an interest in implementing MS-in-QC assays
  • Contract Research Organizations (CROs)
  • Contract Manufacturing Organizations (CMOs)
  • Academic scientists working with protein characterization by MS



FabRICATOR (IdeS) is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.
The enzyme is widely used in characterization, quality control, stability testing, production monitoring and clone selection of antibody-based therapeutics such as mAbs, ADCs, biosimilars and Fc-fusion proteins. FabRICATOR HPLC is used for on-column digestion of IgG below the hinge.
Learn More About FabRICATOR HPLC