NEW! We’re Launching TransGLYCIT™ Remodeling Afucosylated Man5!

We are proudly launching a new format of our popular TransGLYCIT technology! With TransGLYCIT Remodeling Afucosylated Man5, it is possible to generate human IgG with fully homogenous Man5 glycoforms within a few hours!
 
TransGLYCIT is a platform technology that enables efficient and site-specific human IgG glycan remodeling or conjugation. With TransGLYCIT, antibodies with defined and homogenous glycoforms are generated using fast and robust enzymatic workflows.
 

• Efficient and site-specific human IgG Fc N-glycan remodeling
• Prepares human IgG with homogenous glycoprofiles
• A rapid workflow – transglycosylation is completed within a few hours

Generation of Man5 Glycoforms using TransGLYCIT™

Of the high-mannose N-glycan structures, Man5 is the most common one present on therapeutic antibodies. Antibodies carrying Man5 have shown increased clearance rates compared to those carrying complex N-glycan structures. With TransGLYCIT, defined Man5 glycoforms of therapeutic antibodies can be generated to investigate the impact of this N-glycan structure on functional antibody properties such as serum half-life and clearance.
 
High-mannose N-glycan structures are biosynthetically generated without core fucose. Using the TransGLYCIT platform, trastuzumab was remodeled to carry the Man5 glycoform within three hours. The transglycosylated antibody was analyzed at the subunit level using LC-MS (Fig. 1). The mass spectra show the glycan profile of native trastuzumab (top) and the resulting homogeneity and mass shift after complete transglycosylation of the antibody using TransGLYCIT Remodeling Afucosylated Man5.
 

 
Figure 1. Deconvoluted mass spectra of the scFc fragment of native trastuzumab (top) and after transglycosylation with TransGLYCIT Remodeling Afucosylated Man5 (bottom). The mAb was digested with FabRICATOR and the subunits analyzed by reversed-phase LC-MS on a Waters™ BioAccord™ system equipped with a Waters™ BioResolve RP mAb column (2.1 x 50 mm).
 

TransGLYCIT – Transglycosylation of IgG