Substrate Specificity of FucosEXO®

Performance Activity

Selective removal of α1-2/3/4 fucose for more comprehensive N- and O-glycan characterization.

Fucose is present on both N- and O-glycans in different linkages. α1-2, α1-3, and α1-4 linked fucose most commonly occurs in O-glycans, and as antenna fucosylation of N-glycans, whereas α1-6 linked fucose is found as a modification of the N-glycan core.

We measured release of fucose by FucosEXO from a panel of different oligosaccharide substrates representing the different linkages. FucosEXO efficiently releases α1-2, α1-3, and α1-4 linked fucose, without activity on α1-6 linked fucose residues.

Specificity for α1-2, α1-3, and α1-4 linked fucose

The substrate specificity of FucosEXO. Substrate specificity was analyzed using equal molar amounts of synthetic oligosaccharides; α1-2 (2’- fucosyllactose,) α1-3 (3-fucosyllactose), α1-4 (Lewis a) and α1-6 (α1-6 fucosylated chitobiose). Substrates were incubated with FucosEXO for 30 min at 37°C and the amount of released fucose measured spectrometrically using an L-Fucose Assay Kit (Megazymes).

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