Isotype-specific Digestion using IgMBRAZOR™

Performance Activity

IgM-specific digestion with no off-target cleavage of IgG or IgA, enabling selective generation of Fab’ and Fc fragments.

IgMBRAZOR is a unique IgM-specific enzyme that digests human IgM at one specific site below the CH2 to generate F(ab’)2 and Fc fragments.

Here, all four subclasses of human IgG along with IgA and IgM were incubated with IgMBRAZOR Lyophilized. IgM was successfully digested into F(ab’)2 and Fc fragments in 30 minutes.

Highly specific digestion of IgM

No digestion was observed for any of the IgG subclasses or IgA. This shows the isotype-specificity of IgMBRAZOR towards human IgM antibodies and demonstrates the possibility of using the enzyme in complex samples such as serum, plasma, and blood samples without the risk of off-target digestion.

No digestion observed for any IgG subclass or IgA

Isotype specificity of IgMBRAZOR. Human IgG1 (trastuzumab), IgG2 (panitumumab), IgG3 (from human serum; polyclonal), IgG4 (nivolumab) and IgA (from human serum; polyclonal) were incubated with IgMBRAZOR Lyophilized for 2 h at 37°C. IgM (from human serum; polyclonal) was incubated with IgMBRAZOR Lyophilized for 30 minutes or 2h at 37°C. All samples were analyzed reduced with SDS-PAGE.

Related Products

Resources