IgASAP™
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IgASAP™ Sub1 Lyophilized
Lyophilized enzyme for above hinge digestion of human IgA1
IgASAP is a family of IgA-digesting enzymes. IgASAP Sub1 is an IgA1-specific enzyme that digests human IgA1 at one specific site above the hinge, and IgASAP Sub1+2 specifically digests IgA1 and IgA2m1 above the hinge. Both enzymes generate intact and homogenous Fab and Fc fragments.
IgASAP Sub1 hydrolyzes both secretory and serum IgA1. The digestion is complete within 1 hour, and due to the specificity of the enzyme, there is no risk of overdigestion if the incubation time is prolonged. The enzyme is active at pH 6.5 to 8.0 and does not require reducing conditions or co-factors for activity. The optimal activity is at 37°C, but the digestion can also be performed at room temperature using an increased incubation time.
IgASAP Sub1 is cloned from Streptococcus oralis and expressed in E. coli. The enzyme contains a His-tag and has a molecular weight of 148 kDa.
IgASAP Sub1 Lyophilized is available as a lyophilized powder in 1000 unit vials for digestion of 1 mg IgA1.
Why IgASAP™ Sub1 Lyophilized?
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
IgASAP™ Sub1 Lyophilized 1000 units
Lyophilized enzyme for above hinge digestion of 1 mg human IgA1
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit IgASAP Sub1 digests ≥ 90% of 1 µg human IgA1 when incubated in PBS (10 mM sodium phosphate, 140 mM NaCl, 2.7 mM KCl), pH 7.4 at 37°C for 1 hour.
Content and Storage
IgASAP Sub1 Lyophilized is reconstituted by the addition of water. No preservatives added. After reconstitution, the enzyme is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and the content should be stored at -20°C upon arrival.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Applications
Site-specific digestion of human IgA1, generating homogeneous Fab and Fc fragments for high-resolution middle-level LC-MS characterization.
Subclass selective digestion of IgA in complex samples like serum, enabling targeted analysis of IgA1 and IgA2m1 without off-target cleavage.
Performance Activity
Highly specific digestion of IgA, with no activity on IgG or IgM, enabling targeted middle-level characterization of IgA1 and IgA2 subclasses.
FAQ and Support
Which buffers are compatible with IgASAP Sub1?
The enzyme is active at pH 6.5 to 8.0, tolerates up to 300 mM NaCl, and does not require reducing conditions or co-factors for activity. PBS and TBS buffers are both compatible. Optimization may be required if buffers other than the recommended are used.
Does IgASAP Sub1 digest human IgA1-only?
IgASAP Sub1 may digest IgA1 from other primate species with similar hinge sequences, but this has not been tested. IgASAP Sub1 does not digest IgA2.
Can the IgASAP Sub1 digestion be performed at lower temperatures?
The optimal activity is at 37°C, but the digestion can also be performed at room temperature using a prolonged incubation time.
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