GalactEXO®
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GalactEXO® Lyophilized
Lyophilized enzyme for hydrolysis of β1-3,4-linked galactose
GalactEXO is a β-Galactosidase mix that hydrolyses β1-3,4-linked terminal galactose on N- and O-glycosylated proteins or oligosaccharides.
GalactEXO Lyophilized is available as a lyophilized powder in 2000 unit vials for hydrolysis of β1-3,4-linked galactose on 2 mg glycoprotein.
Why GalactEXO® Lyophilized?
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
GalactEXO® Lyophilized 2000 units
Lyophilized enzyme for hydrolysis of β1-3,4-linked galactose on 2 mg glycoprotein
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit of GalactEXO Lyophilized hydrolyzes galactoses from >95% of 0.15 nmol Gal-β1,3-GalNAc-pNP and 2 nmol Gal-β1,4-GlcNAc-pNP when incubated in 20 mM Tris pH 6.8 at 37°C for 30 min. 1 unit of GalactEXO Lyophilized will hydrolyze the galactose of 1 μg of glycoprotein.
Content and Storage
GalactEXO Lyophilized is reconstituted by addition of water. No preservatives added. After reconstitution, GalactEXO Lyophilized is stable for 1 month at +4-8°C. The product is shipped cold and should be stored at -20°C upon arrival.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
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Applications
Removal of glycan β-galactose to simplify IgG glycan heterogeneity, improve LC-MS analysis, and reveal underlying glycation modifications.
FAQ and Support
Does GalactEXO digest fucosylated glycan structures such as Lewis X or Lewis a?
No. Branching fucose linked to the same residue as the β-galactose inhibits GalactEXO activity and needs to be removed prior to GalactEXO treatment. The FucosEXO enzyme can be used for hydrolysis of α1-2, α1-3 and α1-4 linked fucose.
Can you combine GalactEXO with SialEXO or SialEXO 23 to remove both galactoses and sialic acids in a single reaction?
Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.
How long is the reaction time for GalactEXO?
For most substrates such as antibodies or Fc-fusion proteins a 2 h incubation results in hydrolysis of over 95% of the present galactose. More complex substrates with high degree of galactosylation may require longer incubation times.
Does GalactEXO hydrolyze galactose on N and O-glycosylated proteins?
Yes, both beta1-3 linked galactose on O-glycans and beta1-4 galactose on N-glycans are hydrolyzed by GalactEXO.
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