FabRICATOR® MagIC

Immobilized enzyme on magnetic beads for automated digestion of IgG below the hinge

Available Products

  • Immobilized

  • Magnetic

FabRICATOR (IdeS) is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.

The FabRICATOR MagIC magnetic beads contains FabRICATOR Immobilized enzyme. The beads enable fast automated digestion of multiple antibodies in parallel in a middle-level characterization workflow. They are designed for automation platforms, but perform equally well on a shaking table if automation is not available.

After digestion, the samples can be directly transferred to the autosampler of the LC-MS for fast chromatography or to another analytical method for analysis. Automated parallel antibody digestion and subunit generation allow for the processing of the numerous samples associated with clone selection, process development and stability studies for a potential biopharmaceutical. Automated antibody subunit analysis reduces the operator time and the risk for sample handling errors, and also increases the throughput.

Why FabRICATOR® MagIC?

  • No enzyme in the final sample
  • Fast, automated sample preparation
  • Digestion of up to 96 samples in parallel
  • Applicable to all magnetic automation platforms

Available Products

  • FabRICATOR® MagIC 2 ml

    Immobilized enzyme on magnetic beads for automated digestion of IgG below the hinge

    A0-FRM-024

    €792.00

    Buy / Request a Quote

    • FabRICATOR® MagIC 4 x 2 ml

      Immobilized enzyme on magnetic beads for automated digestion of IgG below the hinge

      A0-FRM-096

      €2,665.00

      Buy / Request a Quote

      For information on how to order, visit Place an Order or contact us directly at order@genovis.com.

      Product Information

      Product Overview

      Product size: 2 ml and 4 x 2 ml
      Sample size: Digestion of up to 24 x 200 µg IgG (2ml) and 96 x 200 µg IgG (4 x 2 ml)
      Short Specification: 20µL settled magnetic agarose resin digest >=95% of 20 and 200µg human IgG1 in 100µL 10 mM Sodium Phosphate, 150 mM NaCl pH 7.4 in 20 minutes at 37°C as monitored by SDS-PAGE
      Starting material: Human IgG1-4, Fc-fusion proteins
      Operating temperature: 37°C
      Storage conditions: +4°-8°C
      Bead material: Magnetic agarose beads
      Compatible Additives: 5mM TECP for generation of reduced subunits, Fd’, LC and scFc.
      0.05% Polysorbate 20 in the digestion buffer to minimize shear stress in automated systems.

      Performance

      The performance of FabRICATOR MagIC at 37°C is typically less than 20 minute digestion with efficiency of >95% for 20-200 µg human IgG1 (1 mg/ml) sample in PBS, pH 7.4.

      Content and Storage

      FabRICATOR MagIC is supplied as a 25% bulk resin in vials. The product is shipped cold and should be stored at +4-8°C.

      Resources

      FAQ and Support

      FabRICATOR MagIC is suitable for using in automated systems that can handle magnetic beads. On the applications page you can read about how it’s used in a KingFisher™ workflow.

      The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme.

      We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.

      FabRICATOR is a cysteine protease. It should not result in any other modifications than the digestion of the peptide bond. Digestion will be between the two Glycines (…CPAPELLG / GPSVF…) and the C-terminus will regain the carboxyl group – that is addition of one OH-group and the N-terminal amino group will be regained (addition of one H).

      In summary, R1-CO-NH-R2 + H2O –> R1-COOH + NH2-R2.

      The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme.

      Find all FAQ here

      Legal & Disclaimers

      All rights reserved. Genovis products may be covered by one or more patents, trademarks and copyrights owned by Genovis AB or licensed from third parties. For more information about commercial rights, please contact the Genovis team at licensing@genovis.com.

      Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.

      The trademarks Thermo Scientific™, KingFisher™ and BindIt™ are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

      © Genovis AB

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