IgGZERO™
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Lyophilized enzyme with low levels of endotoxin for hydrolysis of complex-type Fc N-glycans

IgGZERO (EndoS) is an IgG-specific endoglycosidase hydrolyzing complex N-glycans at the Fc N-glycosylation sites.
IgGZERO Low Endotoxin is available as a lyophilized powder in 2000 unit vials for deglycosylation of 2 mg IgG. This product formulation has low levels of endotoxins per vial (< 0.2 EU).
Lyophilized enzyme with low levels of endotoxin for deglycosylation of 2 mg IgG

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One unit deglycosylates ≥ 95% of 1μg human IgG when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30 min.
IgGZERO Low Endotoxin is reconstituted by addition of water. No preservatives added. After reconstitution, IgGZERO Low Endotoxin is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
A simple and rapid estimation of IgG core fucosylation using LC-MS analysis.
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