FabRICATOR®
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FabRICATOR® Low Endotoxin
Lyophilized enzyme with low levels of endotoxin for below hinge digestion of IgG
FabRICATOR (IdeS) is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.
FabRICATOR Low Endotoxin is available as a lyophilized powder in two different sizes; 2000 and 5000 units, for digestion of 2 or 5 mg IgG, respectively. This product formulation has low levels of endotoxins per vial, with <0.04 EU /vial for the 2000 unit vials and <0.10 EU/vial for the 5000 unit vials.
Why FabRICATOR® Low Endotoxin?
- Efficient digestion with documented low levels of endotoxin
- Suitable for use in sensitive in vivo or cell-based assays
Available Products
FabRICATOR® Low Endotoxin 2000 units
Lyophilized enzyme with low levels of endotoxin for below hinge digestion of 2 mg IgG
FabRICATOR® Low Endotoxin 5000 units
Lyophilized enzyme with low levels of endotoxin for below hinge digestion of 5 mg IgG
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit digests ≥ 95% of 1 µg human IgG when incubated in 10 mM sodium phosphate, 137 mM NaCl, 2.7 mM KCl pH 7.4 at 37°C for 30 min.
Buffers
The buffers below have been tested for compatibility with FabRICATOR digestion at different pH. Note that the FabRICATOR enzyme is irreversibly inactivated below pH 5.0. Digestion above pH 8.0 requires prolonged/optimized conditions.
- Phosphate buffer saline (PBS) 6.0 – 8.0
- Tris buffer 7.0 – 8.0
- MES buffer 5.5 – 6.5
- HEPES buffer 7.0 – 8.0
- Ammonium bicarbonate buffer 6.0 – 7.0
- Sodium acetate buffer 6.0
Content and Storage
The FabRICATOR Low Endotoxin is reconstituted by addition of water. No preservatives added. After reconstitution, it is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
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Instruction for Use
Product Specifications
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