GingisREX™
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GingisREX™ Lyophilized
Lyophilized enzyme for digestion C-terminally of arginine residues
GingisREX (RgpB) is an arginine-specific protease that digests proteins C-terminally of arginine residues, including Arg-Pro linkages that are difficult to digest with other enzymes.
GingisREX Lyophilized is available as a lyophilized powder in vials of 5 μg enzyme.
Why GingisREX™ Lyophilized?
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
GingisREX™ Lyophilized 5 ug
Lyophilized enzyme for arginine-specific protein digestion. Processes 0.1-1 mg protein.
GingisREX™ Lyophilized 5 x 5 ug
Lyophilized enzyme for arginine-specific protein digestion. Processes 5 x 0.1-1 mg protein.
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Quality Control Assay
The activity and specificity of GingisREX is tested on oxidized insulin β-chain at a protease:substrate ratio of 1:20 and 1:200.
The digestion reaction is performed at 37°C for 30 min and 18 h and analysed with RP-HPLC.
Buffers
To maintain the enzymatically active cysteine in a reduced form, TCEP is added to preserve the reducing environment (see user instructions below for details).
GingisREX is active in a broad pH range, 5.0-9.0, with optimal activity between pH 6.5-8.0. Digestion can be performed in the presence of chaotropic agents or detergents such as urea, SDS, tween and SDC. Buffers tested and shown to be compatible with GingisREX are Tris, Bis-Tris and ammonium bicarbonate.
Content and Storage
GingisREX Lyophilized is reconstituted by addition of water. No preservatives added.
GingisREX is shipped cold and should be stored at -20°C upon arrival. After reconstitution, the enzyme is stable for at least 1 month at 4-8°C.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »GingisREX™ for Characterization of Lysine Conjugated ADCs
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GlyCLICK® for Generation and Analysis of ADCs
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GingisREX™ for Specific Digestion at Arginine Residues
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Applications
Arginine-specific peptide mapping with improved perfomance over Arg-C, generates larger peptides complementary to trypsin.
Performance Activity
Highly specific arginine-specific protease with limited off-target digestion, enabling clean peptide maps and high-confidence LC-MS data analysis.
Citations
Powered by BiozFAQ and Support
Is the reaction ready in only 60 minutes?
The digestion time is affected by many factors like the enzyme to sample ratio, pH of the digestion reaction, and the sample characteristics. The reaction time needs to be optimized for each specific case.
Is cysteine really needed for activation of the cysteine protease if TCEP or DTT is added in the reaction?
Yes, cysteine is required for both activity and specificity of the GingisREX enzyme.
Can guanidinium hydrochloride be used as a denaturing agent instead of urea?
No, GingisREX is inhibited by GdHCl even in low concentrations. This chaotrope should be avoided.
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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