SialEXO™
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SialEXO™ Immobilized
Immobilized enzymes for desialylation of glycoproteins in spin columns
The SialEXO products are sialidases for the removal and analysis of sialic acids. The enzymes are active on both N- and O-linked glycans present on native glycoproteins or released glycan structures.
The SialEXO Immobilized spin columns contain SialEXO enzyme covalently coupled to agarose beads, for desialylation of glycoproteins without contaminating the final preparation with enzyme. It hydrolyzes sialic acids (α2-3, α2-6 and α2-8) on native glycoproteins and released glycans.
The Microspin columns are available for desialylation of 2 x 0.5 mg, 5 x 0.5 mg, or 10 x 0.5 mg glycoprotein.
Why SialEXO™ Immobilized?
- Easy-to-use spin columns
- Increased enzyme-to-substrate ratio for improved digestion efficiency
- No enzyme in the final sample
Available Products
SialEXO™ Immobilized Microspin 2 x 0.5 mg
Immobilized enzymes for desialylation of 2 x 0.5 mg glycoprotein in spin columns
SialEXO™ Immobilized Microspin 5 x 0.5 mg
Immobilized enzymes for desialylation of 5 x 0.5 mg glycoprotein in spin columns
SialEXO™ Immobilized Microspin 10 x 0.5 mg
Immobilized enzymes for desialylation of 10 x 0.5 mg glycoprotein in spin columns
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Activity
0.1 ml of settled SialEXO Immobilized agarose resin hydrolyzes sialic acids from > 90% of 0.5 mg C1 inhibitor in 30 min at room temperature in PBS pH 7.4 as monitored by ion exchange chromatography.
One Microspin column contains sufficient SialEXO-coupled agarose beads to desialylate 0.5 mg glycoprotein.
Content and Storage
SialEXO Immobilized is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »SialEXO™ for Complete and Rapid Desialylation of Therapeutic Glycoproteins
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Applications
Simplified charge variant analysis by removing sialic acid-driven heterogeneity to reveal underlying protein profile.
Performance Activity
Complete desialylation of complex glycoproteins to simplify glycan profiles and enable more confident protein characterization.
FAQ and Support
Can I use SialEXO for desialylation of both N- and O-glycans?
Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.
Does SialEXO require denaturation of the proteins for activity on glycoproteins?
No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.
Can I use SialEXO in combination with PNGaseF?
Yes, both enzymes are active under native conditions, preferably use 20 mM Tris pH 7. If denaturing reaction conditions are required for PNGaseF, the reaction with SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.
Can I use SialEXO in combination with OglyZOR or OpeRATOR?
Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.
Does SialEXO contain a purification tag?
Yes, SialEXO contains a 6x His-tag.
Legal & Disclaimers
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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