FabRICATOR® Z
Download Product Folder
FabRICATOR® Z Fab2 Kit
Immobilized enzyme and affinity resin for below hinge digestion of mouse IgG2a and IgG3 and purification of fragments
FabRICATOR Z (IdeZ) is a cysteine protease that digests mouse IgG2a and IgG3 at a specific site below the hinge, generating a homogenous pool of F(ab’)2 and Fc fragments after a two-hour incubation.
FabRICATOR Z Fab2 Kit consists of spin columns of FabRICATOR Z Immobilized for antibody digestion, and spin columns of CaptureSelect™* Fc resin for purification of fragments. One FabRICATOR Z spin column contains sufficient FabRICATOR Z-coupled agarose beads to process 0.5 mg mouse IgG2a and IgG3.
Why FabRICATOR® Z Fab2 Kit?
- Purification of homogenous F(ab’)2 fragments from mouse IgG2a and IgG3
- Fast – pure fragments in under two hours
- Reliable workflow for preparative applications
Available Products
FabRICATOR® Z Fab2 Kit Microspin 0.5 mg
Immobilized enzyme and affinity resin for below hinge digestion of 0.5 mg mouse IgG2a and IgG3 and purification of fragments
FabRICATOR® Z Fab2 Kit Microspin 5 x 0.5 mg
Immobilized enzyme and affinity resin for below hinge digestion of 5 x 0.5 mg mouse IgG2a and IgG3 and purification of fragments
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Activity
One spin column contains sufficient FabRICATOR Z-coupled agarose beads to digest 0.5 mg mouse IgG2a or IgG3.
Content and Storage
The FabRICATOR Z Immobilized spin columns and CaptureSelect™* spin columns are supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the products!
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »FAQ and Support
Can I re-use FabRICATOR Z Immobilized columns?
We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.
Can I release Fc fragments from the CaptureSelect column after isolating the F(ab’)2 fragments?
It is possible to elute the Fc fragments from the CaptureSelect column using a low pH buffer, for example a 0.1M Glycine buffer pH=3.0. The pH must immediately after elution be adjusted to neutral pH by adding 0.1x elution volume of 1M Tris pH=8.0.
Legal & Disclaimers
All rights reserved. Genovis products may be covered by one or more patents, trademarks and copyrights owned by Genovis AB or licensed from third parties. For more information about commercial rights, please contact the Genovis team at licensing@genovis.com.
Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
* Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.
© Genovis AB