GingisKHAN™
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GingisKHAN™ Lyophilized
Lyophilized enzyme for above hinge digestion of human IgG1
GingisKHAN (Kgp) is a cysteine protease that digests human IgG1 at a single site above the hinge, generating intact and homogenous Fab and Fc fragments.
GingisKHAN Lyophilized is available as a lyophilized powder in 2000 unit vials for digestion of up to 2 mg human IgG1. The enzyme requires mildly reducing conditions to be for active, and is provided together with 5 vials of lyophilized GingisKHAN Reducing Agent.
Why GingisKHAN™ Lyophilized?
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
GingisKHAN™ Lyophilized 2000 units
Lyophilized enzyme for above hinge digestion of 2 mg human IgG1
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit GingsKHAN Lyophilized digests ≥ 95% of 1μg human IgG1 when incubated in 0.1 M Tris, pH 8.0 at 37°C for 1 hour.
Content and Storage
GingisKHAN Lyophilized is reconstituted by addition of water. No preservatives added. It is supplied with 5 vials of lyophilized GingisKHAN Reducing Agent (10x), containing 20 mM cysteine upon reconstitution.
GingisKHAN Lyophilized and GingisKHAN Reducing Agent are shipped cold and should be stored at -20°C upon arrival.
After reconstitution, the GingisKHAN enzyme is stable for at least 1 month at +4-8°C. Reconstituted GingisKHAN Reducing Agent cannot be stored – use it freshly prepared!
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
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What is the difference between FabALACTICA and GingisKHAN?
FabALACTICA (IgdE) and GingisKHAN (Kgp) are both cysteine proteases that digest IgG1 above the hinge to generate intact Fab and Fc fragments. FabALACTICA is specific for human IgG1 and digests at a specific site (…KSCDKT / HTCPPCP…) with overnight incubation without the need for reducing conditions. GingisKHAN is a general protease that with the recommended reaction conditions, 1 hour with light reducing conditions, digests human IgG1 at a specific site (…KSCDK/THTCPPCP…).
How can I stop the GingisKHAN reaction?
The enzyme is a cysteine protease that can be inhibited by alkylating agents such as iodoacetamide. There are also several other known inhibitors to this enzyme, like KYT-36.
Can you recommend a protocol for purification of the Fab?
Genovis provides a GingisKHAN Fab kit containing a specific Capture Select CH1 resin for further purification of the Fab. See this page for more information.
Can I use GingisKHAN on other subclasses than human IgG1?
No. GingisKHAN is a protease that digests at exposed Lysines with no secondary structure restrictions. At the recommended reaction conditions, GingisKHAN digestion above the hinge has only been confirmed on human IgG1 with the exposed lysine above the hinge, (…KSCDK / THTCPPCP…). No digestion has been confirmed on other subclasses of human IgG except for several digestion sites in human IgG3.
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