FabALACTICA™
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FabALACTICA™ Lyophilized
Lyophilized enzyme for above hinge digestion of human IgG1
FabALACTICA (IgdE) is a cysteine protease that digests human IgG1 at a specific site above the hinge, generating intact and homogenous Fab and Fc fragments.
FabALACTICA Lyophilized is available as a lyophilized powder in 2000 unit vials for digestion of 2 mg human IgG1.
Why FabALACTICA™ Lyophilized?
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
FabALACTICA™ Lyophilized 2000 units
Lyophilized enzyme for above hinge digestion of 2 mg human IgG1
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit digests ≥ 90% of 1 μg human IgG1, when incubated in 150 mM sodium phosphate, pH 7.0 at 37°C overnight (16-18 h).
Content and Storage
FabALACTICA Lyophilized is reconstituted by addition of water. No preservatives added. After reconstitution, the enzyme is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »AppNote FabALACTICA™ for Paired Glycan Analysis
Download Application Notes
SmartEnzymes™ for Generation of Homogeneous Antibody Fragments
Download Scientific Poster
Performance Activity
Simple preparation of highly pure Fab fragments from IgG1 antibodies using FabALACTICA digestion combined with Fc affinity purification.
Citations
Powered by BiozFAQ and Support
Can I digest Fc-fusion proteins using FabALACTICA (IgdE)?
No, FabALACTICA has shown very poor activity on Fc-fusion proteins.
What is the difference between FabALACTICA and GingisKHAN?
FabALACTICA (IgdE) and GingisKHAN (Kgp) are both cysteine proteases that digest IgG1 above the hinge to generate intact Fab and Fc fragments. FabALACTICA is specific for human IgG1 and digests at a specific site (…KSCDKT / HTCPPCP…) with overnight incubation without the need for reducing conditions. GingisKHAN is a general protease that with the recommended reaction conditions, 1 hour with light reducing conditions, digests human IgG1 at a specific site (…KSCDK/THTCPPCP…).
Can FabALACTICA and FabRICATOR be used in the same reaction?
Yes, for human IgG1 molecules both enzymes can be used in combination to generate a specific hinge peptide. The incubation time is significantly longer for FabALACTICA and it is recommended to first run the FabALACTICA digest and then add FabRICATOR for the last 30 min.
We want to make Fab’s directly from antibodies. When would the selection of FabALACTICA be the first choice over GingisKHAN or FabULOUS?
FabALACTICA does not require reducing conditions as both GingisKHAN and FabULOUS does, and allows for generation of Fab fragments from native human IgG1.
Can I release Fc fragments from the CaptureSelect column after isolating the Fab fragments?
It is not recommended to elute the Fc fragments from the CaptureSelect column since the fraction might also include undigested or semi digested material.
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