GlycoCATCH™ Affinity Purification Microspin 0.2 mg
Enrichment resin for affinity purification of 0.2 mg mucin-type O-glycoproteins and peptides
GlycOCATCH is an affinity resin for purification of mucin-type O-glycosylated proteins and peptides. The affinity resin is based on inactive OpeRATOR® enzyme that has been engineered to bind O-glycosylated proteins and peptides with high affinity.
GlycOCATCH is designed to specifically bind O-glycosylated proteins and peptides. The affinity resin is based on inactive OpeRATOR enzyme
that has been engineered to bind core 1 and core 2 type O-GalNAc glycosylated proteins and peptides with high affinity. GlycOCATCH is provided in a spin-column format to allow easy-to-use enrichment of O-glycoproteins. Due to the strong interaction between the GlycOCATCH resin and O-glycoproteins/ peptides, the elution is performed using 8 M urea or competitive release using active OpeRATOR enzyme (included). For optimal performance, the sialic acid content
of the glycoprotein will need to be removed using the included SialEXO enzyme.
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