Tell us about your project!
In the current work, we utilized FabRICATOR (IdeS) digestion to streamline the characterization of a very complex antibody (trastuzumab)-oligonucleotide conjugate (AOC). Both denaturing and native Orbitrap mass spectrometry approaches were employed to analyze the corresponding intermediate and final AOC samples at intact and subunit levels.
How did SmartEnzymes enhance your project?
FabRICATOR has proven its ease of use, efficiency, and excellent specificity! Actually, FabRICATOR-based workflows have been an essential part of the Spectroswiss’ mAb analysis toolbox for almost a decade. We were the first to use FabRICATOR for the electron transfer dissociation (ETD) middle-down mass spectrometry of mAbs in 2014 (https://pubs.acs.org/doi/10.1021/ac4036857). Today, we regularly use FabRICATOR in our research and CRO activities for the mAb and ADC/AOC analysis that we perform on several structural levels.
Publication
Blog post
Discover the SmartEnzymes used in this project!
