Tell us about your project and how SmartEnzymes enhanced it
F(ab)2 workflows have for years been an essential part of Symphogen’s characterization toolbox. With the introduction of FabRICATOR MagIC we have made a swift transition to automated, fast and robust F(ab)2 production. Combining the FabRICATOR MagIC with the KingFisher Prime Duo robot we can now produce F(ab)2 subunits in less than 30 minutes, and with minimal manual sample handling. Indeed, the only time we handle the antibody sample is when we pipet the sample into the 96 well plate, where the digestion is performed subsequently. In other words, the FabRICATOR MagIC workflow is truly a one-step pipetting workflow. The rest is handled by the FabRICATOR MagIC and the KingFisher Prime Duo robot.
After the digestion, the 96 well plate with the digested mAbs is transferred directly to autosampler of our LC MS platform and analyzed. Simple, robust, hands-off and superfast production of F(ab)2 subunits for analytical characterization of antibody biopharmaceuticals.
Have ideas for new SmartEnzymes and what they could be called? Tell us more:
MagIC versions of Fab generating enzymes and enzymes for deglycosylation of mAbs.
