Tell us about yourself and ImaGene-iT
I am CSO at ImaGene-iT, an independent contract research company supporting the life science industry and academia. Our projects comprise a variety of questions and involve various types of biological samples on both the histological and cellular level. As a principal investigator, I perform most of the quality assessments using high-resolution confocal microscopy along with imaging for further digital analysis.
What are the key aspects of experimental design?
From the question at hand and to obtain the best end result, we want to participate in the whole chain of tailor-made procedures. This includes the initial handling of samples, the choice of labeling technology and the best suited detection and imaging equipment.
What are the advantages of using GlyCLICK?
In our tests, antibodies with GlyCLICK-conjugated fluorophores work very well for imaging in both in vitro cell and tissue analyses. The conjugates provide an excellent signal-to-noise ratio for detection and imaging with fluorescence microscopy. For confocal microscope analysis, GlyCLICK-conjugated antibodies give an optimal range of intensity levels. The optimal range of detected intensity levels improves the quality of assessment as well as the digital imaging.
What does the future of imaging look like?
In our field, one of the major recent advances in imaging is the ability to extract a large amount of data from images. The use of markers with a known number of conjugation sites per target molecule, such as the GlyCLICK technology offers, can significantly improve quantitation possibilities for all types of imaging-based analysis. The combined use of GlyCLICK-conjugated fluorescence with small animal in vivo imaging could further expand the quality of quantitative data that can be collected for our clients, from the cellular level to the whole animal.
