GingisKHAN™
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GingisKHAN™ Fab Kit
Lyophilized enzyme and affinity resin for above hinge digestion of human IgG1 and purification of Fab fragments
GingisKHAN (Kgp) is a cysteine protease that digests human IgG1 at a single site above the hinge, generating intact and homogenous Fab and Fc fragments.
GingisKHAN Fab Kit contains one vial of lyophilized GingisKHAN, five vials of GingisKHAN Reducing Agent for antibody digestion, and four microspin columns of CaptureSelect™* CH1 resin for affinity purification of the Fab fragments.
The CaptureSelect™ IgG-CH1 affinity matrix recognizes the CH1 domain of human IgG antibodies, enabling purification of Fab fragments independent on the light-chain isotype. Due to its unique selectivity for the CH1 domain, no co-purification of free light-chain contaminants will occur. Each CaptureSelect™ microspin column contains sufficient material to purify 0.5 mg human IgG1.
Why GingisKHAN™ Fab Kit?
- Purification of homogeneous Fab fragments from human IgG1
- Reliable workflow for preparative applications
- Enables chain pairing characterization of bispecific antibodies
Available Products
GingisKHAN™ Fab Kit 2 mg
Lyophilized enzyme and affinity resin for above hinge digestion of 2 mg human IgG1 and purification of fragments
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit of GingisKHAN enzyme digests ≥ 95% of 1μg human IgG1 when incubated in 0.1 M Tris, pH 8.0 at 37°C for 1h.
Content and Storage
GingisKHAN Fab Kit is shipped cold. The GingisKHAN Lyophilized vials and GingisKHAN Reducing Agent should be stored at -20°C upon arrival.
The microspin columns containing CaptureSelect™* IgG-CH1 resin should be stored at +4-8°C. Do not freeze the spin columns!
After reconstitution, the GingisKHAN enzyme is stable for 2 months at +4-8°C. Reconstituted GingisKHAN Reducing Agent cannot be stored – use it freshly prepared!
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Applications
Efficient Fab generation for SPR-based separation of affinity and avidity effects, supporting detailed functional characterization of monoclonal antibodies.
Citations
Powered by BiozFAQ and Support
Can you recommend a protocol for purification of the Fab?
Genovis provides a GingisKHAN Fab kit containing a specific Capture Select CH1 resin for further purification of the Fab. See this page for more information.
Is GingisKHAN an IgG specific protease?
No, GingisKHAN is a general protease that with the recommended reaction conditions, 1 hour with light reducing conditions, digests human IgG1 at one specific site (…KSCDK/THTCPPCP…) above the hinge, generating Fab and Fc.
Can I use another reducing agent besides GingisKHAN reducing agent?
We recommend using the 10x reducing agent provided with GingisKHAN which has an optimized pH to provide full enzymatic activity without affecting the disulphide bridges between the light chain and Fd region of the heavy chain, keeping the Fab intact. If other reducing agent is used there is a risk for non-optimal reaction conditions. It is important to use freshly prepared Reducing Agent (max storage 6h) for guaranteed activity of GinigsKHAN.
Can I use GingisKHAN on other subclasses than human IgG1?
No. GingisKHAN is a protease that digests at exposed Lysines with no secondary structure restrictions. At the recommended reaction conditions, GingisKHAN digestion above the hinge has only been confirmed on human IgG1 with the exposed lysine above the hinge, (…KSCDK / THTCPPCP…). No digestion has been confirmed on other subclasses of human IgG except for several digestion sites in human IgG3.
How can I stop the GingisKHAN reaction?
The enzyme is a cysteine protease that can be inhibited by alkylating agents such as iodoacetamide. There are also several other known inhibitors to this enzyme, like KYT-36.
Legal & Disclaimers
All rights reserved. Genovis products may be covered by one or more patents, trademarks and copyrights owned by Genovis AB or licensed from third parties. For more information about commercial rights, please contact the Genovis team at licensing@genovis.com.
Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
* Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.
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