Question 1

Is FabRICATOR stable in the presence of denaturing agents such as guanidine hydrochloride or SDS?

kk · Accepted Answer

We have tested 4-6 M GuHCl which the enzyme does not withstand without significant decrease of activity. SDS have not been evaluated but 0.1% RapiGest from waters have negative influence on activity. Taken together the enzyme does not well tolerate denaturing agents although we have not tested this in more detailed studies.

Question 2

Is FabRICATOR stable in the presence of denaturing agents such as guanidine hydrochloride or SDS?

kk · Accepted Answer

We have tested 4-6 M GuHCl which the enzyme does not withstand without significant decrease of activity. SDS have not been evaluated but 0.1% RapiGest from waters have negative influence on activity. Taken together the enzyme does not well tolerate denaturing agents although we have not tested this in more detailed studies.

Question 3

Is FabRICATOR stable in the presence of denaturing agents such as guanidine hydrochloride or SDS?

kk · Accepted Answer

We have tested 4-6 M GuHCl which the enzyme does not withstand without significant decrease of activity. SDS have not been evaluated but 0.1% RapiGest from waters have negative influence on activity. Taken together the enzyme does not well tolerate denaturing agents although we have not tested this in more detailed studies.