Does FabRICATOR introduce modifications?

FabRICATOR belongs to the cysteine protease family. There should be no modification other than the cleavage of the peptide bond between the two glycines. The c-terminal will regain the carboy group – that is addition of one OH-group and the N-terminal amino group will be regained (addition of one H).

In summary, R1-CO-NH-R2 + H2O –> R1-COOH + NH2-R2

Order