Question 1

Does FabRICATOR introduce modifications?

kk · Accepted Answer

FabRICATOR is a cysteine protease. It should not result in any other modifications than the digestion of the peptide bond. Digestion will be between the two Glycines (...CPAPELLG / GPSVF...) and the C-terminus will regain the carboxyl group - that is addition of one OH-group and the N-terminal amino group will be regained (addition of one H).

In summary, R1-CO-NH-R2 + H2O --> R1-COOH + NH2-R2.

Question 2

Does FabRICATOR introduce modifications?

kk · Accepted Answer

FabRICATOR is a cysteine protease. It should not result in any other modifications than the digestion of the peptide bond. Digestion will be between the two Glycines (...CPAPELLG / GPSVF...) and the C-terminus will regain the carboxyl group - that is addition of one OH-group and the N-terminal amino group will be regained (addition of one H).

In summary, R1-CO-NH-R2 + H2O --> R1-COOH + NH2-R2.

Question 3

Does FabRICATOR introduce modifications?

kk · Accepted Answer

FabRICATOR is a cysteine protease. It should not result in any other modifications than the digestion of the peptide bond. Digestion will be between the two Glycines (...CPAPELLG / GPSVF...) and the C-terminus will regain the carboxyl group - that is addition of one OH-group and the N-terminal amino group will be regained (addition of one H).

In summary, R1-CO-NH-R2 + H2O --> R1-COOH + NH2-R2.