Can I use FabRICATOR (IdeS) with very low amounts of IgG?

There should not be any problems to use low concentrations of IgG. If you don’t get complete digestion, try to increase the incubation time. There is no risk of over digestion and the incubation time can be prolonged to over night if necessary.

An alternative would be to increase the ratio enzyme to antibody (i.e. Follow protocol for mouse IgG). We have not made any specific tests on low concentrations but we have not heard that it would be any problems.

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