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2022-12-12
Wael Bahnan, Lotta Happonen , Hamed Khakzad, Vibha Kumra Ahnlide, Therese de Neergaard , Sebastian Wrighton , Oscar Andre1, Eleni Bratanis , Di Tang , Thomas Hellmark , Lars Björck , Oonagh Shannon, Lars Malmström, Johan Malmström & Pontus Nordenfelt - Challenges and Strategies for a Thorough Characterization of Antibody Acidic Charge Variants
2022-11-03
Y. Diana Liu , Lance Cadang, Karenna Bol, Xiao Pan, Katherine Tschudi, Mansour Jazayri, Julien Camperi , David Michels, John Stults, Reed J. Harris and Feng Yang - Discriminating cross-reactivity in polyclonal IgG1 responses against SARS-CoV-2 variants of concern
2022-10-15
Danique M. H. van Rijswijck, Albert Bondt, Max Hoek, Karlijn van der Straten, Tom G. Caniels, Meliawati Poniman, Dirk Eggink, Chantal Reusken, Godelieve J. de Bree, Rogier W. Sanders, Marit J. van Gils & Albert J. R. Heck - Mechanistic insights into the rational design of masked antibodies
2022-06-25
Carolina T Orozco, Manuela Bersellini, Lorraine M Irving, Wesley W Howard, David Hargreaves, Paul W A Devine, Elise Siouve, Gareth J Browne, Nicholas J Bond, Jonathan J Phillips,, Peter Ravn, Sophie E Jackson - Combination of IM-Based Approaches to Unravel the Coexistence of Two Conformers on a Therapeutic Multispecific mAb
2022-05-23
Evolène Deslignière, Simon Ollivier, Anthony Ehkirch, Armelle Martelet, David Ropartz, Nelly Lechat, Oscar Hernandez-Alba, Jean-Michel Menet, Séverine Clavier, Hélène Rogniaux, Bruno Genet, and Sarah Cianférani - Structures of activin ligand traps using natural sets of type I and type II TGFb receptors
2022-01-21
Erich J. Goebel, Chandramohan Kattamuri, Gregory R. Gipson, Lavanya Krishnan, Moises Chavez, Magdalena Czepnik, Michelle C. Maguire, Rosa Grenha, Maria Håkansson, Derek T. Logan, Asya V. Grinberg, Dianne Sako, Roselyne Castonguay, Ravindra Kumar, and Thomas B. Thompson - Systematic analysis of human antibody response to ebolavirus glycoprotein reveals high prevalence of neutralizing public clonotypes
2022-01-13
Elaine C. Chen, Pavlo Gilchuk, Seth J. Zost, Philipp A. Ilinykh, Elad Binshtein, Kai Huang, Luke Myers, Stefano Bonissone, Samuel Day, Chandrahaas R. Kona, Andrew Trivette, Joseph X. Reidy, Rachel E. Sutton, Christopher Gainza, Summer Monroig, Edgar Davidson, Erica Ollmann Saphire, Benjamin J. Doranz, Natalie Castellana, Alexander Bukreyev, Robert H. Carnahan, James E. Crowe Jr - Human plasma IgG1 repertoires are simple, unique, and dynamic
2021-09-16
Albert Bondt, Max Hoek, Sem Tamara, Olaf L. Cremer, Marc J.M. Bonten, Albert J.R. Heck - The monoclonal antibody combination REGEN-COV protects against SARS-CoV-2 mutational escape in preclinical and human studies
2021-05-28
Richard Copin, Alina Baum, Elzbieta Wloga, Kristen E. Pascal, Stephanie Giordano, Benjamin O. Fulton, Anbo Zhou, Nicole Negron, Kathryn Lanza, Newton Chan, Angel Coppola, Joyce Chiu, Min Ni, Yi Wei, Gurinder S. Atwal, Annabel Romero Hernandez, Kei Saotome, Yi Zhou, Matthew C. Franklin, Andrea T. Hooper, Shane McCarthy, Sara Hamon, Jennifer D. Hamilton, Hilary M. Staples, Kendra Alfson, Ricardo Carrion, Jr., Shazia Ali, Thomas Norton, Selin Somersan-Karakaya, Sumathi Sivapalasingam, Gary A. Herman, David M. Weinreich, Leah Lipsich, Neil Stahl, Andrew J. Murphy, George D. Yancopoulos, Christos A. Kyratsous - Structural insight into SARS-CoV-2 neutralizing antibodies and modulation of syncytia
2021-04-19
Daniel Asarnow, Bei Wang, Wen-Hsin Lee, Yuanyu Hu, Ching-Wen Huang, Bryan Faust, Patricia Miang Lon Ng, Eve Zi Xian Ngoh, Markus Bohn, David Bulkley, Andrés Pizzorno, Beatrice Ary, Hwee Ching Tan, Chia Yin Lee, Rabiatul Adawiyah Minhat, Olivier Terrier, Mun Kuen Soh, Frannie Jiuyi Teo, Yvonne Yee Chin Yeap, Shirley Gek Kheng Seah, Conrad En Zuo Chan, Emily Connelly, Nicholas J. Young, Sebastian Maurer-Stroh, Laurent Renia, Brendon John Hanson, Manuel Rosa- Calatrava, Aashish Manglik, Yifan Cheng, Charles S. Craik, Cheng-I Wang - Multiattribute Monitoring of Antibody Charge Variants by Cation- Exchange Chromatography Coupled to Native Mass Spectrometry
2021-03-09
Markus Haberger, Anna-Katharina Heidenreich, Michaela Hook, Jürgen Fichtl, Rainer Lang, Florian Cymer, Mahdi Adibzadeh, Felix Kuhne, Harald Wegele, Dietmar Reusch, Lea Bonnington and Patrick Bulau - Generating Informative Sequence Tags from Antigen-Binding Regions of Heavily Glycosylated IgA1 Antibodies by Native Top-Down Electron Capture Dissociation
2021-02-11
Jean-Francois Greisch, Maurits A. den Boer, Frank Beurskens, Janine Schuurman, Sem Tamara, Albert Bondt, Albert J. R. Heck - C-terminal lysine clipping of IgG1: impact on binding to human FcγRIIIa and neonatal Fc receptors
2021-01-18
Valegh Faid, Yann Leblanc, Marie Berger, Alexander Seifert, Nicolas Bihoreau, Guillaume Chevreux - Selectivity over coverage in de novo sequencing of IgGs
2020-10-06
Maurits A. den Boer, Jean-Francois Greisch, Sem Tamara, Albert Bondt, Albert J. R. Heck - Intact and subunit-specific analysis of bispecific antibodies by sheathless CE-MS
2020-08-21
Christoph Gstöttner, Simone Nicolardi, Markus Haberger, Dietmar Reusch, Manfred Wuhrer, Elena Domínguez-Vegaa - The impact of proline isomerization on antigen binding and the analytical profile of a trispecific anti-HIV antibody
2020-01-10
Alessandro Masiero, Lechat Nelly, Gentric Marianne, Sourrouille Christophe, Laville Florian, Crépin Ronan, Borel Claire, Ziegler Cornelia, Bisch Grégoire, Leclerc Eric, Laurent Ludovic, Brault Dominique, Alexandre Sylvie, Gagnaire Marie, Duffieux Francis, Soubrier Fabienne, Capdevila Cécile, Arnould Isabelle, Dumas Jacques, Dabin Jérôme, Genet Bruno, Radošević Katarina, Menet Jean-Michel & Prades Catherine - A generic method for intact and subunit level characterization of mAb charge variants by native mass spectrometry
2019-09-23
Y. Leblanc, V. Faid, M.A. Lauber, Q. Wang, N. Bihoreau, G. Chevreux - Characterization of therapeutic antibody fragmentation using automated capillary western blotting as an orthogonal analytical technique
2019-07-04
Yunxiao Zhu, Deepti Ahluwalia, Yingchen Chen, Madesh Belakavadi, Amit Katiyar, Tapan K. Das - Chromatographic behavior of bivalent bispecific antibodies on cation exchange columns. II. Biomolecular perspectives
2019-04-09
Lucas K. Kimerer, Timothy M. Pabst, Alan K. Hunter, Giorgio Carta - Application of a label-free and domain-specific free thiol method in monoclonal antibody characterization
2019-03-25
Yi Pu, Yunqiu Chen, Tai Nguyen, Chong-Feng Xu, Li Zang, Zoran Sosic, Tyler Carlage - Characterization and analysis of scFv-IgG bispecific antibody size variants
2018-07-25
Mingyan Cao, Chunlei Wang, Wai Keen Chung, Dana Motabar, Jihong Wang, Elizabeth Christian, Shihua Lin, Alan Hunter, Xiangyang Wang & Dengfeng Liu - A three-point identity criteria tool for establishing product identity using icIEF method
2018-03-01
Deepti Ahluwalia⁎, Madesh Belakavadi, Tapan K. Das, Amit Katiyar - Middle-up analysis of monoclonal antibodies after combined IgdE and IdeS hinge proteolysis: Investigation of free sulfhydryls
2017-11-21
Valegh Faid, Yann Leblanc, Nicolas Bihoreau, Guillaume Chevreux
- Capillary liquid chromatography coupled with mass spectrometry for analysis of nanogram protein quantities on a wide-pore superficially porous particle column in top-down proteomics
2023-01-01
Laurent Lignieres, Véronique Legros, Manel Khelil, Nicolas Senecaut, Matthew A Lauber, Jean-Michel Camadro, Guillaume Chevreux - Development, validation, and implementation of a robust and quality control-friendly focused peptide mapping method for monitoring oxidation of co-formulated monoclonal antibodies
2022-11-29
Chengdong Xu, Sumee Khanal, Nicholas A Pierson, Jorge Quiroz, Brent Kochert, Xiaoyu Yang, David Wylie, Christopher A Strulson - Challenges and Strategies for a Thorough Characterization of Antibody Acidic Charge Variants
2022-11-03
Y. Diana Liu , Lance Cadang, Karenna Bol, Xiao Pan, Katherine Tschudi, Mansour Jazayri, Julien Camperi , David Michels, John Stults, Reed J. Harris and Feng Yang - A Combination of Native LC-MS Approaches for the Comprehensive Characterization of the Antibody-Drug Conjugate Trastuzumab Deruxtecan
2022-10-26
Evolène Deslignière, Hélène Diemer, Stéphane Erb, Pierre Coliat, Xavier Pivot, Alexandre Detappe, Oscar Hernandez-Alba, Sarah Cianférani - Development of a 64Cu-labeled CD4+ T cell targeting PET tracer: evaluation of CD4 specificity and its potential use in collagen-induced arthritis
2022-09-16
Anne Skovsbo Clausen, Camilla Christensen, Esben Christensen, Sigrid Cold, Lotte Kellemann Kristensen, Anders Elias Hansen & Andreas Kjaer - Expediting the chromatographic analysis of COVID-19 antibody therapeutics with ultra-short columns, retention modeling and automated method development
2022-09-12
Bastiaan Duivelshof, Arnold Zöldhegyi, Davy Guillarme, Matthew Lauber, Szabolcs Fekete - Microchip electrophoresis separation coupled to mass spectrometry (MCE-MS) for the rapid monitoring of multiple quality attributes of monoclonal antibodies
2022-08-26
Seth Madren, Linda Yi - Implementing 1.5 mm internal diameter columns into analytical workflows
2022-06-10
Benjamin P. Libert, Justin M. Godinho, Samuel W. Foster, James P. Grinias, Barry E. Boyes - Cysteine metabolic engineering and selective disulfide reduction produce superior antibody-drug-conjugates
2022-05-04
Renée Procopio-Melino, Frank W. Kotch, Amar S. Prashad, Jose M. Gomes, Wenge Wang, Bo Arve, Andrew Dawdy, Lawrence Chen, Justin Sperry, Christine Hosselet, Tao He, Ronald Kriz, Laura Lin, Kimberly Marquette, Lioudmila Tchistiakova, Will Somers, Jason C. Rouse & Xiaotian Zhong - Coupling Anion Exchange Chromatography with Native Mass Spectrometry for Charge Heterogeneity Characterization of Monoclonal Antibodies
2022-04-14
Anita P. Liu, Yuetian Yan, Shunhai Wang, and Ning Li - Therapeutic antibody glycosylation impacts antigen recognition and immunogenicity
2022-04-13
Babette Wolf, Mateusz Piksa, Isabelle Beley, Agnes Patoux, Thierry Besson, Valerie Cordier, Bernd Voedisch, Patrick Schindler, Daniela Stöllner, Ludovic Perrot, Stephan von Gunten, Dominique Brees, Michael Kammüller - Correlative N-glycan and charge variant analysis of cetuximab expressed in murine, chinese hamster and human expression systems
2022-02-17
Anne Trappe, Florian Füssl, Silvia Millán-Martín, Rachel Ronan, Izabella Zaborowska, Jonathan Bones - Deciphering the High Viscosity of a Therapeutic Monoclonal Antibody in High Concentration Formulations by Microdialysis-Hydrogen/Deuterium Exchange Mass Spectrometry
2022-01-07
Xiaobin Xu, Aming Zhang, Yingjie Xu, Yuan Cao, Stephen Cale, Matthew Franklin, Prasad Sarangapani, Peter M.Ihnat, Haibo Qiu, Dingjiang Liu, Erica A.Pyles, Mohammed Shameem, Ning Li - A New Practice to Monitor the Fabrication Process of Fab-Targeting Ligands from Bevacizumab by LC-MS: Preparation and Analytical Characterization
2022-01-04
Franck Marquet, Valentina D’Atri, Davy Guillarme and Gerrit Borchard - Development of a sub-hour on-line comprehensive cation exchange chromatography x RPLC method hyphenated to HRMS for the characterization of lysine-linked antibody-drug conjugates
2021-12-25
Soraya Chapel, Florent Rouvière, Pierre Guibal, Delphine Mathieu, Sabine Heinisch - Next generation Fc scaffold for multispecific antibodies
2021-12-17
Bram Estes, Athena Sudom, Danyang Gong, Douglas A. Whittington, Vivian Li, Christopher Mohr, Danqing Li, Timothy P. Riley, Stone D.-H. Shi, Jun Zhang, Fernando Garces, and Zhulun Wang - Intra-articular delivery of full-length antibodies through the use of an in situ forming depot
2021-12-13
Alexis Fayd'herbe De Maudave, Wilhem Leconet, Karine Toupet, Michael Constantinides, Guillaume Bossis, Marionde Toledo, Jérôme Vialaret, Christophe Hirtz, Adolfo Lopez-Noriega, Christian Jorgensen, Daniele Noël, Pascale Louis-Plence, Sylvestre Grizot, Martin Villalba - Post-Column Denaturation-Assisted Native Size-Exclusion Chromatography–Mass Spectrometry for Rapid and In-Depth Characterization of High Molecular Weight Variants in Therapeutic Monoclonal Antibodies
2021-11-17
Yuetian Yan, Tao Xing, Anita P. Liu, Zhengqi Zhang, Shunhai Wang, and Ning Li - Elevated Fab glycosylation of anti-hinge antibodies
2021-11-02
J Koers, NIL Derksen, WJJ Falkenburg, P Ooijevaar-de Heer, MT Nurmohamed, GJ Wolbink & T Rispens - Systematic workflow for studying domain contributions of bispecific antibodies to selectivity in multimodal chromatography
2021-10-23
Siddharth S. Parasnavis, Ben Niu, Matthew Aspelund, Wai K. Chung, Mark Snyder, Steven M. Cramer - Quantitative N-Glycan Profiling of Therapeutic Monoclonal Antibodies Performed by Middle-Up Level HILIC-HRMS Analysis
2021-10-20
Bastiaan L. Duivelshof, Steffy Denorme, Koen Sandra, Xiaoxiao Liu, Alain Beck, Matthew A. Lauber, Davy Guillarme and Valentina D’Atri - Side-by-Side Comparison of uPAR-Targeting Optical Imaging Antibodies and Antibody Fragments for Fluorescence-Guided Surgery of Solid Tumors
2021-10-12
Victor M. Baart, Labrinus van Manen, Shadhvi S. Bhairosingh, Floris A. Vuijk, Luisa Iamele, Hugo de Jonge, Claudia Scotti, Massimo Resnati, Robert A. Cordfunke, Peter J. K. Kuppen, Andrew P. Mazar, Jacobus Burggraaf, Alexander L. Vahrmeijer & Cornelis F. M. Sier - Inline electrochemical reduction of NISTmAb for middle-up subunit liquid chromatography-mass spectrometry analysis
2021-09-29
Tomos E. Morgan, Craig Jakes, Hendrik-Jan Brouwer, Silvia Millán-Martín, Jean-Pierre Chervet, Ken Cook, Sara Carillo and Jonathan Bones - Ultra-short ion-exchange columns for fast charge variants analysis of therapeutic proteins
2021-09-22
Jose Antonio Navarro-Huerta, Amarande Murisier, Jennifer M. Nguyen, Matthew A. Lauber, Alain Beck, Davy Guillarme, Szabolcs Fekete - Drug-to-Antibody Ratio Estimation via Proteoform Peak Integration in the Analysis of Antibody–Oligonucleotide Conjugates with Orbitrap Fourier Transform Mass Spectrometry
2021-09-14
Konstantin O. Nagornov, Natalia Gasilova, Anton N. Kozhinov, Pasi Virta, Patrik Holm, Laure Menin, Victor J. Nesatyy, and Yury O. Tsybin - Characterization of N-Terminal Glutamate Cyclization in Monoclonal Antibody and Bispecific Antibody Using Charge Heterogeneity Assays and Hydrophobic Interaction Chromatography
2021-09-10
Mingyan Cao, Niluka De Mel, Jihong Wang, Conner Parthemore, Yang Jiao, Weimin Chen, Shihua Lin, Dengfeng Liu, Greg Kilby, Xiaoyu Chen - Bispecific antibody characterization by a combination of intact and site-specific/chain-specific LC/MS techniques
2021-09-03
Bastiaan L.Duivelshof, Alain Beck, Davy Guillarme, Valentina D'Atri - Analytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture of TNF-α
2021-08-17
Etienne Laborie, Victor Le-Minh, Thanh Duc Mai, Mehdi Ammar, Myriam Taverna & Claire Smadja - Evaluation of strategies for overcoming trifluoroacetic acid ionization suppression resulted in single-column intact level, middle-up, and bottom-up reversed-phase LC-MS analyses of antibody biopharmaceuticals
2021-05-12
Maria A. Khalikova, Laimonas Skarbalius, Denis K. Naplekov, Siddharth Jadeja, František Švec, Juraj Lenčo - In-capillary (electrophoretic) digestion-reduction-separation: A smart tool for middle-up analysis of mAb
2021-05-03
Meriem Dadouch, Yoann Ladner, Claudia Bich, Jérôme Montels, Jacques Morel, Cherine Bechara, Catherine Perrin - Chromatographic and Adsorptive Behavior of a Bivalent Bispecific Antibody and Associated Fragments
2021-04-27
Lucas K. Kimerer, Ben Niu, Timothy M. Pabst, Weiguo Zhai, Alan K. Hunter, Giorgio Carta - Automated and Faster Affinity Capture Method for Biotransformation Assessment of Site-Specific Antibody Drug Conjugates
2021-03-22
Aarti Jashnani, Srikanth Kotapati, Madhura Deshpande, Sayumi Yamazoe, Pavel Strop, Arvind Rajpal and Gavin Dollinger - Application of middle-down approach in quantitation and catabolite identification of protein by LC–high-resolution mass spectrometry
2021-03-15
Lijuan Kang, Shengsheng Xu, Yongle Pang, Tom Kirchner, Yue-mei Zhang, Wilson Edwards, Raul Camacho, Lisa Norquay, Naidong Weng & Wenying Jian - Alternative mobile phase additives for the characterization of protein biopharmaceuticals in liquid chromatography – Mass spectrometry
2021-02-24
Honorine Lardeux, Bastiaan L.Duivelshof, OlivierColas, AlainBeck, David V.McCalley, DavyGuillarme, ValentinaD’Atri - Electron Transfer Dissociation Parameter Optimization Using Design of Experiments Increases Sequence Coverage of Monoclonal Antibodies
2021-02-17
Milos Cejkov, Tyler Greer, Reid O’Brien Johnson, Xiaojing Zheng and Ning Li - Microdroplet Ultrafast Reactions Speed Antibody Characterization
2021-02-05
Pengyi Zhao, Harsha P. Gunawardena, Xiaoqin Zhong, Richard N. Zare and Hao Chen - C-terminal lysine clipping of IgG1: impact on binding to human FcγRIIIa and neonatal Fc receptors
2021-01-18
Valegh Faid, Yann Leblanc, Marie Berger, Alexander Seifert, Nicolas Bihoreau, Guillaume Chevreux - Cysteine in cell culture media induces acidic IgG1 species by disrupting the disulfide bond network
2020-11-17
Elke Prade, Anne Zeck, Fabian Stiefel, Andreas Unsoeld, David Mentrup, Erik Arango-Gutierrez, Ingo H. Gorr - Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural Heterogeneity
2020-10-29
Wenwen Zhu, Menglin Li, Jinlan Zhang - Use of Ultra-short Columns for Therapeutic Protein Separations, Part 2: Designing the Optimal Column Dimension for Reversed- Phase Liquid Chromatography
2020-10-07
Szabolcs Fekete, Amarande Murisier, Jennifer M. Nguyen, Michael J. Bolton, Jonathan Belanger, Alain Beck, Jean-Luc Veuthey, Kevin Wyndham, Matthew A. Lauber, and Davy Guillarme - Selectivity over coverage in de novo sequencing of IgGs
2020-10-06
Maurits A. den Boer, Jean-Francois Greisch, Sem Tamara, Albert Bondt, Albert J. R. Heck - Towards automation of Collision Induced Unfolding experiments through online Size Exclusion Chromatography coupled to native Mass Spectrometry
2020-09-04
Evolène Deslignière, Anthony Ehkirch, Thomas Botzanowski, Alain Beck, Oscar Hernandez-Alba, and Sarah Cianférani - Improved N- and C-terminal sequencing of proteins by combining positive and negative ion MALDI in-source decay mass spectrometry
2020-08-17
Simone Nicolardi, David Peter Alexander Kilgour, Yuri E.M. van der Burgt, Manfred Wuhrer - IgG-cleaving endopeptidase enables in vivo gene therapy in the presence of anti-AAV neutralizing antibodies
2020-06-01
Christian Leborgne, Elena Barbon, Jeffrey M. Alexander, Hayley Hanby, Sandrine Delignat, Daniel M. Cohen, Fanny Collaud, Saghana Muraleetharan, Dan Lupo, Joseph Silverberg, Karen Huang, Laetitia van Wittengerghe, Béatrice Marolleau, Adeline Miranda, Anna Fabiano, Victoria Daventure, Heena Beck, Xavier M. Anguela, Giuseppe Ronzitti, Sean M. Armour, Sebastien Lacroix-Desmazes & Federico Mingozzi - Middle Level IM–MS and CIU Experiments for Improved Therapeutic Immunoglobulin Subclass Fingerprinting
2020-05-26
Thomas Botzanowski, Oscar Hernandez-Alba, Martine Malissard, Elsa Wagner-Rousset, Evolène Deslignière, Olivier Colas, Jean-François Haeuw, Alain Beck, and Sarah Cianférani - Distinctive binding properties of human monoclonal LGI1 autoantibodies determine pathogenic mechanisms
2020-05-21
Melanie Ramberger, Antonio Berretta, Jeanne M M Tan, Bo Sun, Sophia Michael, Tianrong Yeo, Jakob Theorell, Rachael Bashford-Rogers, Sofija Paneva, Victoria O’Dowd, Neesha Dedi, Sarfaraj Topia, Robert Griffin, Jorge Ramirez-Franco, Oussama El Far, Stéphanie Baulac, Maria I Leite, Arjune Sen, Alexander Jeans, David McMillan, Diane Marshall, Daniel Anthony, Daniel Lightwood, Patrick Waters, Sarosh R Irani - Generation and validation of structurally defined antibody–siRNA conjugates
2020-04-29
Alex R Nanna, Alexander V Kel’in, Christopher Theile, Justin M Pierson, Zhi Xiang Voo, Ashish Garg, Jayaprakash K Nair, Martin A Maier, Kevin Fitzgerald, Christoph Rader - Monitoring CD8a+ T Cell Responses to Radiotherapy and CTLA-4 Blockade Using [64Cu]NOTA-CD8a PET Imaging
2020-02-21
Lotte K. Kristensen, Camilla Christensen, Maria Z. Alfsen, Sigrid Cold, Carsten H. Nielsen & Andreas Kjaer - Combination of intact, middle-up and bottom-up levels to characterize 7 therapeutic monoclonal antibodies by capillary electrophoresis – Mass spectrometry
2020-01-23
Jérémie Giorgetti, Alain Beck, Emmanuelle Leize-Wagner, Yannis-Nicolas François, - Middle-level IM-MS and CIU experiments for improved therapeutic immunoglobulin isotype fingerprinting
2020-01-21
Thomas Botzanowski, Oscar Hernandez-Alba, Martine Malissard, Elsa Wagner-Rousset, Evolène Deslignière, Olivier Colas, Jean-François Haeuw, Alain Beck, Sarah Cianférani - Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo
2020-01-09
Anja Schaffert, Maja Hanic´, Mislav Novokmet, Olga Zaytseva, Jasminka Krištic´, Anja Lux, Lars Nitschke, Matthias Peipp, Marija Pezer, René Hennig, Erdmann Rapp, Gordan Lauc, Falk Nimmerjahn - Implementation of a High-Resolution Liquid Chromatography–Mass Spectrometry Method in Quality Control Laboratories for Release and Stability Testing of a Commercial Antibody Product
2019-12-23
Izabela Sokolowska, Jingjie Mo,Fatie Rahimi Pirkolachahi, Carol McVean, Lars A. T. Meijer, Linda Switzar, Crina Balog, Michael J. Lewis, Ping Hu - Universal Affinity Capture Liquid Chromatography-Mass Spectrometry Assay for Evaluation of Biotransformation of Site-Specific Antibody Drug Conjugates in Preclinical Studies
2019-12-20
Srikanth Kotapati, David Passmore, Sayumi Yamazoe, Rajesh Kishore Kumar Sanku, Qiang Cong, Yam B. Poudel, Naidu S. Chowdari, Sanjeev Gangwar, Chetana Rao, Vangipuram S. Rangan, Pina M. Cardarelli, Shrikant Deshpande, Pavel Strop, Gavin Dollinger, and Arvind Rajpal - Analysis of cetuximab N-Glycosylation using multiple fractionation methods and capillary electrophoresis mass spectrometry
2019-12-07
Csaba Váradi, Craig Jakes, Jonathan Bones - Strong Cation Exchange Ultra-Performance Liquid Chromatography Coupled to UV and MS Detectors Allows Robust and Reproducible Characterization of mAb and ADC Charge Variants
2019-12-05
Michal Kliman, Arnie Aistars, Yutaka Matsuda, Brian A. Mendelsohn - Comparing different domains of analysis for the characterisation of N-Glycans on monoclonal antibodies
2019-11-29
Sara Carillo, Raquel Pérez-Robles, CraigJakes, Meire Ribeiro da Silva, Silvia Millán Martín, Amy Farrell, Natalia Navas, Jonathan Bones - TR1801-ADC: A highly potent cMet antibody-drug conjugate with high activity in patient-derived xenograft models of solid tumors
2019-11-14
Marco Gymnopoulos, Oscar Betancourt, Vincent Blot, Ryo Fujita, Diana Galvan, Vincent Lieuw, Sophie Nguyen, Jeanette Snedden, Christine Stewart, Jose Villicana, Jon Wojciak, Eley Wong, Raul Pardo, Neki Patel, Francois D’Hooge, Balakumar Vijayakrishnan, Conor Barry, John A. Hartley, Philip W. Howard, Roland Newman, Julia Coronella - Monitoring glycation levels of a bispecific monoclonal antibody at subunit level by ultrahigh resolution MALDI FT-ICR mass spectrometry
2019-10-21
Christoph Gstöttner, Dietmar Reusch, Markus Haberger, Irina Dragan, Peter van Veelen, David P. A. Kilgour, Yury O. Tsybin, Yuri E.M. van der Burgt, Manfred Wuhrer & Simone Nicolardi - Unambiguous Sequence Characterization of a Monoclonal Antibody in a Single Analysis Using a Nonspecific Immobilized Enzyme Reactor
2019-10-04
Joshua Hinkle, Robert Anthony D'Ippolito, Maria C. Panepinto, Weihan Wang, Dina L. Bai, Jeffrey Shabanowitz, and Donald F. Hunt - Susceptibility of protein therapeutics to spontaneous chemical modifications by oxidation, cyclization, and elimination reactions
2019-10-01
Luigi Grassi, Chiara Cabrele - A generic method for intact and subunit level characterization of mAb charge variants by native mass spectrometry
2019-09-23
Y. Leblanc, V. Faid, M.A. Lauber, Q. Wang, N. Bihoreau, G. Chevreux - Middle-Down Multi-Attribute Analysis of Antibody- Drug Conjugates with Electron Transfer Dissociation
2019-08-23
Bifan Chen, Ziqing Lin, Yanlong Zhu, Yutong Jin, Eli Larson, Qingge Xu, Cexiong Fu, Zhaorui Zhang, Qunying Zhang, Wayne Pritts, and Ying Ge - In Vivo Surveillance and Elimination of Teratoma-forming Human Embryonic Stem Cells with Monoclonal Antibody 2448 targeting Annexin A2
2019-08-07
Heng Liang Tan, Bao Zhu Tan, Winfred Xi Tai Goh, Simeon Cua, Andre Choo - Forced Degradation Testing as Complementary Tool for Biosimilarity Assessment
2019-07-21
Yan Felix Karl Dyck, Daniel Rehm, Jan Felix Joseph, Karsten Winkler, Volker Sandig, Wolfgang Jabs, Maria Kristina Parr - Utility of High Resolution NMR Methods to Probe the Impact of Chemical Modifications on Higher Order Structure of Monoclonal Antibodies in Relation to Antigen Binding
2019-07-01
Subhabrata Majumder, Andrew Saati, Shibu Philip, Lucy L. Liu, Elaine Stephens, Jason C. Rouse, Arun Alphonse Ignatius - Using bispecific antibodies in forced degradation studies to analyze the structure–function relationships of symmetrically and asymmetrically modified antibodies
2019-06-04
Adam R. Evans, Michael T. Capaldi, Geetha Goparaju, David Colter, Frank F. Shi, Sarah Aubert, Lian-Chao Li, Jingjie Mo, Michael J. Lewis, Ping Hu, Pedro Alfonso & Promod Mehndiratta - Confident monoclonal antibody sequence verification by complementary LC-MS techniques
2019-05-23
Amy Farrell, Sara Carillo, Jonathan Bones, Kai Scheffler, Ken Cook - Two sequential layers of antibody‐mediated control of Legionella pneumophila infection
2019-05-10
Stefan S. Weber, Diana Stoycheva, Falk Nimmerjahn, Annette Oxenius - Tuning selectivity in cation-exchange chromatography applied for monoclonal antibody separations, part 2: evaluation of recent stationary phases
2019-05-09
Amarande Murisiera, Evelin Farsang, Krisztián Horváthb, Matthew Lauberc, Alain Beck, Davy Guillarmea, Szabolcs Feketea - Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
2019-04-24
GE Bergdahl, M Hedström, B Mattiasson - Chromatographic behavior of bivalent bispecific antibodies on cation exchange columns. II. Biomolecular perspectives
2019-04-09
Lucas K. Kimerer, Timothy M. Pabst, Alan K. Hunter, Giorgio Carta - Computer-aided gradient optimization of hydrophilic interaction liquid chromatographic separations of intact proteins and protein glycoforms
2019-04-03
Guusje van Schaick Bob W.J. Pirok Rob Haselberg Govert W. Somsen Andrea F.G. Gargano - Application of a label-free and domain-specific free thiol method in monoclonal antibody characterization
2019-03-25
Yi Pu, Yunqiu Chen, Tai Nguyen, Chong-Feng Xu, Li Zang, Zoran Sosic, Tyler Carlage - N-glycans of complex glycosylated biopharmaceuticals and their impact on protein clearance
2019-03-21
Fabian Higel, Theresa Sandl, Chi-Ya Kao, Nicole Pechinger, Fritz Sörgel, Wolfgang Friess, Florian Wolschina, Andreas Seidl - Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual
2019-03-19
Shelly J. Krebs, Young D. Kwon, Chaim A. Schramm, ..., Merlin L. Robb, Peter D. Kwong, Nicole A. Doria-Rose - IgG Charge: Practical and Biological Implications
2019-03-14
Danlin Yang, Rachel Kroe-Barrett, Sanjaya Singh, Thomas Laue - MHC class II proteins mediate cross-species entry of bat influenza viruses
2019-02-20
Umut Karakus, Thiprampai Thamamongood, Kevin Ciminski, Wei Ran, Sira C. Günther, Marie O. Pohl, Davide Eletto, Csaba Jeney, Donata Hoffmann, Sven Reiche, Jan Schinköthe, Reiner Ulrich, Julius Wiener, Michael G. B. Hayes, Max W. Chang, Annika Hunziker, Emilio Yángüez, Teresa Aydillo, Florian Krammer, Josua Oderbolz, Matthias Meier, Annette Oxenius, Anne Halenius, Gert Zimmer, Christopher Benner, Benjamin G. Hale, Adolfo García-Sastre, Martin Beer, Martin Schwemmle & Silke Stertz - Tuning selectivity in cation-exchange chromatography applied for monoclonal antibody separations, part 1: Alternative mobile phases and fine tuning of the separation
2019-02-19
Evelin Farsang, Amarande Murisier, Krisztián Horvátha, Alain Beck, Róbert Kormányd, Davy Guillarme, Szabolcs Fekete - Persistent Antibody Clonotypes Dominate the Serum Response to Influenza over Multiple Years and Repeated Vaccinations
2019-02-19
Jiwon Lee, Philipp Paparoditis, Andrew P. Horton, Alexander Frühwirth, Jonathan R.McDaniel, Jiwon Jung, Daniel R. Boutz, Dania A. Hussein, Yuri Tanno, Leontios Pappas, Gregory C. Ippolito, Davide Corti, Antonio Lanzavecchia, GeorgeGeorgiou - Direct quantitation of therapeutic antibodies for pharmacokinetic studies using immuno-purification and intact mass analysis
2019-02-08
Lisa A Vasicek, Xin Zhu, Daniel S Spellman & Kevin P Bateman - Fc Sialylation Prolongs Serum Half-Life of Therapeutic Antibodies
2019-01-25
Mathilde Bas, Aurélie Terrier, Emilie Jacque, Aurélie Dehenne, Virginie Pochet-Béghin, Cécile Beghin, Anne-Sophie Dezetter, Gilles Dupont, Anaïs Engrand, Benjamin Beaufils, Philippe Mondon, Nathalie Fournier, Christophe de Romeuf, Sylvie Jorieux, Alexandre Fontayne, Lennart T. Mars and Céline Monnet - A generic workflow for the characterization of therapeutic monoclonal antibodies—application to daratumumab
2019-01-07
Bastiaan L. Duivelshof, Szabolcs Fekete, Davy Guillarme, Valentina D’Atri - Structural Analysis of Monoclonal Antibodies by Ultrahigh Resolution MALDI In-Source Decay FT-ICR Mass Spectrometry
2018-12-20
Yuri E.M. van der Burgt, David P. A. Kilgour, Yury O. Tsybin, Kristina Srzentic,́ Luca Fornelli, Alain Beck, Manfred Wuhrer, and Simone Nicolardi - A generic workflow for the characterization of therapeutic monoclonal antibodies—application to daratumumab
2018-12-19
Bastiaan L. Duivelshof & Szabolcs Fekete & Davy Guillarme & Valentina D’Atri - Orthogonal Middle-up Approaches for Characterization of the Glycan Heterogeneity of Etanercept by Hydrophilic Interaction Chromatography Coupled to High-Resolution Mass Spectrometry
2018-12-04
Valentina D’Atri, Lucie Novaḱova, Szabolcs Fekete, Dwight Stoll, Matthew Lauber, Alain Beck, and Davy Guillarme - Fast and Simple Qualitative/Semi‐Quantitative Analysis of Monoclonal Antibody Mixtures Using Liquid Chromatography–Electrospray Triple Time‐of‐Flight Mass Spectrometry
2018-10-15
Jin‐Ju Byeon Min‐Ho Park Seok‐Ho Shin Young G. Shin - Ultrasensitive Characterization of Charge Heterogeneity of Therapeutic Monoclonal Antibodies Using Strong Cation Exchange Chromatography Coupled to Native Mass Spectrometry
2018-10-03
Yuetian Yan, Anita Liu, Shunhai Wang, Thomas J. Daly, Ning Li - Comprehensive characterization of monoclonal antibody by Fourier transform ion cyclotron resonance mass spectrometry
2018-09-19
Yutong Jin, Ziqing Lin, Qingge Xu, Cexiong Fu, Zhaorui Zhang, Qunying Zhang, Wayne A. Pritts, Ying Ge - Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-down NMR
2018-08-13
Jiangnan Peng, Sharadrao M Patil, David A. Keire, Kang Chen - Heterogeneity assessment of antibody-derived therapeutics at the intact and middle-up level by low-flow sheathless capillary electrophoresis-mass spectrometry
2018-08-12
Rob Haselberg, Thomas De Vijlder, Raimond Heukers, Martine J. Smit, Edwin P. Romijn, Govert W. Somsen, Elena Domínguez-Vega - Systematic analysis of monoclonal antibodies against Ebola virus GP defines features that contribute to protection
2018-08-09
Erica Ollmann Saphire, Sharon L. Schendel, Marnie L. Fusco, Karthik Gangavarapu, Bronwyn M. Gunn, Anna Z. Wec, Peter J. Halfmann, Jennifer M. Brannan, Andrew S. Herbert, Xiangguo Qiu, Kshitij Wagh, Shihua He, Elena E. Giorgi, James Theiler, Kathleen B. J. Pommert, Tyler B. Krause, Hannah L. Turner, Charles D. Murin, Jesper Pallesen, Edgar Davidson, Rafi Ahmed, M. Javad Aman, Alexander Bukreyev, Dennis R. Burton, James E. Crowe Jr., Carl W. Davis, George Georgiou, Florian Krammer, Christos A. Kyratsous, Jonathan R. Lai, Cory Nykiforuk, Michael H. Pauly, Pramila Rijal, Ayato Takada, Alain R. Townsend, Viktor Volchkov, Laura M. Walker, Cheng-I Wang, Larry Zeitlin, Benjamin J. Doranz, Andrew B. Ward, Bette Korber, Gary P. Kobinger, Kristian G. Andersen, Yoshihiro Kawaoka, Galit Alter, Kartik Chandran, John M. Dye - A Role for Fc Function in Therapeutic Monoclonal Antibody-Mediated Protection against Ebola Virus
2018-08-08
Bronwyn M. Gunn, Wen-Han Yu, Marcus M. Karim, Jennifer M. Brannan, Andrew S. Herbert, Anna Z. Wec, Peter J. Halfmann, Marnie L. Fusco, Sharon L. Schendel, Karthik Gangavarapu, Tyler Krause, Xiangguo Qiu, Shinhua He, Jishnu Das, Todd J. Suscovich, Jonathan Lai, Kartik Chandran, Larry Zeitlin, James E. Crowe, Jr., Douglas Lauffenburger, Yoshihiro Kawaoka, Gary P. Kobinger, Kristian G. Andersen, John M. Dye, Erica Ollmann Saphire, Galit Alter - Rapid charge variant analysis of monoclonal antibodies to support lead candidate biopharmaceutical development
2018-07-29
Anne Trappe, Florian Füssl, Sara Carillo, Izabela Zaborowska, Paula Meleady, Jonathan Bones - Characterization and analysis of scFv-IgG bispecific antibody size variants
2018-07-25
Mingyan Cao, Chunlei Wang, Wai Keen Chung, Dana Motabar, Jihong Wang, Elizabeth Christian, Shihua Lin, Alan Hunter, Xiangyang Wang & Dengfeng Liu - Accurate Sequence Analysis of a Monoclonal Antibody by Top- Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques
2018-06-12
Luca Fornelli, Kristina Srzentić, Romain Huguet, Christopher Mullen, Seema Sharma, Vlad Zabrouskov, Ryan T. Fellers, Kenneth R. Durbin, Philip D. Compton, Neil L. Kelleher - Monoclonal antibody sequence assessment using a hybrid quadrupole-Orbitrap mass spectrometer
2018-05-31
Amy Farrell, Sara Carillo, Kai Scheffler, Ken Cook, Jonathan Bones - Development of Comprehensive Online Two-Dimensional Liquid Chromatography/Mass Spectrometry Using Hydrophilic Interaction and Reversed-Phase Separations for Rapid and Deep Profiling of Therapeutic Antibodies
2018-04-04
Dwight R Stoll, David Christopher Harmes, Gregory O. Staples, Oscar G. Potter, Carston T. Dammann, Davy Guillarme, and Alain Beck - Characterization of recombinant monoclonal antibody variants detected by hydrophobic interaction chromatography and imaged capillary isoelectric focusing electrophoresis
2018-03-30
Cory King, Rekha Patel, Gomathinayagam Ponniah, Christine Nowak, Alyssa Neill, Zhenyu Gu, Hongcheng Liu - Hydrophilic Auristatin Glycoside Payload Enables Improved Antibody-Drug Conjugate Efficacy and Biocompatibility
2018-03-22
Tero Satomaa, Henna Pynnönen, Anja Vilkman, Titta Kotiranta, Virve Pitkänen, Annamari Heiskanen, Bram Herpers, Leo S. Price, Jari Helin and Juhani Saarinen - Targeting of embryonic annexin A2 expressed on ovarian and breast cancer by the novel monoclonal antibody 2448
2018-03-02
Simeon Cua, Heng Liang Tan, Wey Jia Fong, Angela Chin, Ally Lau, Vanessa Ding, Zhiwei Song, Yuansheng Yang and Andre Choo - A Polar Sulfamide Spacer Significantly Enhances the Manufacturability, Stability, and Therapeutic Index of Antibody–Drug Conjugates
2018-02-20
Jorge M. M. Verkade, Marloes A. Wijdeven, Remon van Geel, Brian M. G. Janssen, Sander S. van Berkel and Floris L. van Delft - Characterization of an antibody-drug conjugate by hydrophilic interaction chromatography coupled to mass spectrometry
2018-02-17
Valentina D’Atri, Szabolcs Fekete, Dwight Stoll, Matthew Lauber, Alain Beck, Davy Guillarme - Practical approaches for overcoming challenges in heightened characterization of antibody-drug conjugates with new methodologies and ultrahigh-resolution mass spectrometry
2018-02-02
Olga V. Friese, Jacquelynn N. Smith, Paul W. Brown & Jason C. Rouse - Parsimonious Charge Deconvolution for Native Mass Spectrometry
2018-01-29
Marshall Bern, Tomislav Caval, Yong J Kil, Wilfred Tang, Christopher Becker, Eric Carlson, Doron Kletter, K. Ilker Sen, Nicolas Galy, Dominique Hagemans, Vojtech Franc, and Albert J.R. Heck - IdeS-cleaved mAb subunit analysis with LC-HRAM-MS: a quick and accurate comparison of biosimilar and originator biotherapeutics
2018-01-01
Sara Carillo, Craig Jakes, Izabela Zaborowska, Jonathan Bones, National Institute for Bioprocessing Research and Training (NIBRT) - Subunits analysis approach for the determination of fucosylation levels in monoclonal antibodies using LC-HRAM-MS
2018-01-01
Craig Jakes, Sara Carillo, Izabela Zaborowska, and Jonathan Bones National Institute for Bioprocessing Research and Training (NIBRT) - Middle-up analysis of monoclonal antibodies after combined IgdE and IdeS hinge proteolysis: Investigation of free sulfhydryls
2017-11-21
Valegh Faid, Yann Leblanc, Nicolas Bihoreau, Guillaume Chevreux - Protein de novo sequencing by top-down and middle-down MS/MS: Limitations imposed by mass measurement accuracy and gaps in sequence coverage
2017-11-20
Lidong He, Chad R. Weisbrod, Alan G. Marshall - Massive immune response against IVIg interferes with response against other antigens in mice: A new mode of action?
2017-10-12
Laetitia Sordé, Sebastian Spindeldreher, Ed Palmer, Anette Karle - Identification and Quantification of Signal Peptide Variants in an IgG1 Monoclonal Antibody Produced in Mammalian Cell Lines
2017-08-19
Yunping Huang, Jinmei Fu, Richard Ludwig, Li Tao, Jacob Bongers, Li Ma, Ming Yao, Mingshe Zhu, Tapan Das, Reb Russell - Preclinical Characterization of CTL-1, A Biosimilar Anti-EGFR Monoclonal Antibody for Cetuximab
2017-08-10
Peisheng Hu, Ryan Gra, Long Zheng, Leslie A Khawli and Alan L Epstein - A Generic HPLC Method for Absolute Quantification of Oxidation in Monoclonal Antibodies and Fc-Fusion Proteins Using UV and MS Detection
2017-06-28
Christof Regl, Therese Wohlschlager, Johann Holzmann and Christian G. Huber - Current possibilities of liquid chromatography for the characterization of antibody-drug conjugates
2017-06-24
Balázs Bobály, Sandrine Fleury-Souverain, Alain Beck, Jean-Luc Veuthey, Davy Guillarme, Szabolcs Fekete - Discovery and Characterization of Histidine Oxidation Initiated Cross-links in an IgG1 Monoclonal Antibody
2017-06-21
Chong-Feng Xu, Yunqiu Chen, Linda Yi, Tim Brantley, Brad Stanley, Zoran Sosic, and Li Zang - Protocols for the analytical characterization of therapeutic monoclonal antibodies. II – Enzymatic and chemical sample preparation
2017-06-19
Balazs Bobaly, Valentina D’Atri, Alexandre Goyon, Olivier Colas, Alain Beck, Szabolcs Fekete, Davy Guillarme - Analysis of recombinant monoclonal antibodies in hydrophilic interaction chromatography: A generic method development approach
2017-06-11
Balázs Bobály, Valentina D’Atri, Alain Beck, Davy Guillarme, Szabolcs Fekete - Stable Isotope Quantitative N-Glycan Analysis by Liquid Separation Techniques and Mass Spectrometry
2017-05-14
Stefan Mittermayr, Simone Albrecht, Csaba Váradi, Silvia Millán-Martín, and Jonathan Bones - High resolution top-down experimental strategies on the Orbitrap platform
2017-03-31
Kai Scheffler, Rosa Viner, Eugen Damoc - Orthogonal liquid chromatography–mass spectrometry methods for the comprehensive characterization of therapeutic glycoproteins, from released glycans to intact protein level
2017-03-27
Eric Largy, Fabrice Cantais, Géry Van Vyncht, Alain Beck, Arnaud Delobel - Comparability study of Rituximab originator and follow-on biopharmaceutical
2017-03-19
Othman Montacir, Houda Montacir, Murat Eravci, Andreas Springer, Stephan Hinderlich, Amirhossein Saadati, Maria Kristina Parr - Analysis of Monoclonal Antibodies in Human Serum as a Model for Clinical Monoclonal Gammopathy by Use of 21 Tesla FT-ICR Top-Down and Middle-Down MS/MS
2017-02-28
Lidong HeLissa C. AndersonDavid R. BarnidgeDavid L. MurrayChristopher L. HendricksonAlan G. Marshall - Top-down and middle-down approach by fraction collection enrichment using off-line capillary electrophoresis – mass spectrometry coupling: Application to monoclonal antibody F c/2 charge variants
2017-02-27
Michael Biacchi, Nassur Said, Alain Beck, Emmanuelle Leize-Wagner, Yannis-Nicolas François, - Development of a fast workflow to screen the charge variants of therapeutic antibodies
2017-02-26
Elsa Wagner-Rousset, Szabolcs Fekete, Laura Morel-Chevillet, Olivier Colas, Nathalie Corva ̈ıa, Davy Guillarme, Alain Beck - Rapid assessment of oxidation via middle-down LCMS correlates with methionine side-chain solvent-accessible surface area for 121 clinical stage monoclonal antibodies
2017-02-14
Rong Yang, Tushar Jain, Heather Lynaugh, R. Paul Nobrega, Xiaojun Lu, Todd Boland, Irina Burnina, Tingwan Sun, Isabelle Caffry, Michael Brown, Xiaoyong Zhi, Asparouh Lilov & Yingda Xu - Subunit mass analysis for monitoring antibody oxidation
2017-01-20
Izabela Sokolowska, Jingjie Mo, Jia Dong, Michael J. Lewis, Ping Hu - Highly sensitive glycosylamine labelling of O-glycans using non-reductive β-elimination
2017-01-14
Kenichiro Furuki, Toshimasa Toyo’oka & Kazutoshi Ban - Guiding bispecific monovalent antibody formation through proteolysis of IgG1 single-chain
2017-01-05
Nazzareno Dimasi, Ryan Fleming, Kris F. Sachsenmeier, Binyam Bezabeh, Carl Hay, Jincheng Wu, Erin Sult, Saravanan Rajan, Li Zhuang, Peter Cariuk, Andrew Buchanan, Michael A. Bowen, Herren Wu & Changshou Gao - Anti-Hinge Antibodies Recognize IgG Subclass- and Protease-Restricted Neoepitopes
2017-01-01
Willem J. J. Falkenburg, Dirkjan van Schaardenburg, Pleuni Ooijevaar-de Heer, Michel W. P. Tsang-A-Sjoe,Irene E. M. Bultink, Alexandre E. Voskuyl, Arthur E. H. Bentlage, Gestur Vidarsson, Gertjan Wolbink, and Theo Rispens - Characterization of charge variants of a monoclonal antibody using weak anion exchange chromatography at subunit levels
2016-12-23
G. Ponniah, C. Nowak, A. Neill & H. Liu - Structural analysis of variable domain glycosylation of anti-citrullinated protein antibodies in rheumatoid arthritis reveals the presence of highly sialylated glycans.
2016-12-12
Lise Hafkenscheid, Albert Bondt, Hans U. Scherer, Tom W.J. Huizinga, Manfred Wuhrer, René E.M. Toes and Yoann Rombouts - Custom-Designed Affinity Capture LC-MS F(ab')2 Assay for Biotransformation Assessment of Site-Specific Antibody Drug Conjugates
2016-10-25
D. Su, C. Ng, M. Khosraviani, SF. Yu, E. Cosino, S. Kaur and K. Xu - Quantitative twoplex glycan analysis using 12C6 and 13C6 stable isotope 2-aminobenzoic acid labelling and capillary electrophoresis mass spectrometry
2016-09-23
Csaba Váradi, Stefan Mittermayr, Silvia Millán-Martín and Jonathan Bones - Orthogonal Assessment of Biotherapeutic Glycosylation: A Case Study Correlating N-Glycan Core Afucosylation of Herceptin with Mechanism of Action
2016-09-12
Rosie Upton, Leonard Bell, Colin Guy, Paul Caldwell, Sian Estdale, Perdita E. Barran and David Firth - Target-independent variable region mediated effects on antibody clearance can be FcRn independent
2016-09-09
Ryan L. Kelly, Yao Yu, Tingwan Sun, Isabelle Caffry, Heather Lynaugh, Michael Brown, Tushar Jain, Yingda Xu & K. Dane Wittrup - Nonclinical Evaluation of PF-06438179: A Potential Biosimilar to Remicade® (Infliximab)
2016-09-01
Mazin Derzi, Theodore R. Johnson, Ahmed M. Shoieb, Hugh D. Conlon, Penny Sharpe, Andrew Saati, Sarah Koob, Michael W. Bolt, Leslie G. Lorello, Jim McNally, Carol F. Kirchhoff, Teresa A. Smolarek and Michael W. Leach - Antibodies elicited by the first non-viral prophylactic cancer vaccine show tumor-specificity and immunotherapeutic potential
2016-08-22
Jason J. Lohmueller, Shuji Sato, Lana Popova, Isabel M. Chu, Meghan A. Tucker, Roberto Barberena, Gregory M. Innocenti, Mare Cudic, James D. Ham, Wan Cheung Cheung, Roberto D. Polakiewicz, Olivera J. Finn - High-Throughput, Automated Protein A Purification Platform with Multiattribute LC-MS Analysis for Advanced Cell Culture Process Monitoring
2016-08-03
Jia Dong, Nicole Migliore, Steven J. Mehrman, John Cunningham, Michael J. Lewis and Ping Hu - Comparison of originator and biosimilar therapeutic monoclonal antibodies using comprehensive two-dimensional liquid chromatography coupled with time-of-flight mass spectrometry.
2016-06-30
Matthew Sorensen, David C. Harmes, Dwight R. Stoll, Gregory O. Staples, Szabolcs Fekete, Davy Guillarme & Alain Beck - Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure
2016-06-07
Kang Chen Dianna S. Long Scott C. Lute Michaella J. Levy Kurt A. Brorson David A. Keire - A hingeless Fc fusion system for site-specific cleavage by IdeS
2016-05-21
Shabazz Novarra, Luba Grinberg, Keith W. Rickert, Arnita Barnes, Susan Wilson & Manuel Baca - Lipid- and polyion complex-based micelles as agonist platforms for TNFR superfamily receptors
2016-05-16
Ryan N. Gilbreth, Shabazz Novarra, Leslie Wetzel, Stelios Florinas, Horacio Cabral, Kazunori Kataoka, Jonathan RiosDoria, Ronald J. Christie, Manuel Baca - Rapid and improved characterization of therapeutic antibodies and antibody related products using IdeS digestion and subunit analysis
2016-05-04
Jonathan Sjögren, Fredrik Olsson and Alain Beck - Fc-fusion mimetics
2016-04-29
H. Khalili, P. T. Khaw and S. Brocchini - Characterization of cysteinylation and trisulfide bonds in a recombinant monoclonal antibody.
2016-04-26
Adriana Kita, Gomathinayagam Ponniah, Christine Nowak, and Hongcheng Liu - Potential of Hydrophilic Interaction Chromatography for the Analytical Characterization of Protein Biopharmaceuticals
2016-04-19
Aur´elie Periat Szabolcs Fekete Alessandra Cusumano Jean-Luc Veuthey Alain Beck Matthew Lauber Davy Guillarme - Characterization of the Co-elution of Host Cell Proteins with monoclonal antibodies during protein a purification
2016-04-16
Qingchun Zhang*, Andrew M. Goetze, Huanchun Cui, Jenna Wylie, Ben Tillotson, Art Hewig, Michael P. Hall and Gregory C. Flynn - Characterization of mAb dimers reveals predominant dimer forms common in therapeutic mAbs
2016-03-31
Friederike Plath, Philippe Ringler, Alexandra Graff-Meyer, Henning Stahlberg, Matthias E. Lauer, Arne C. Rufer, Melissa A. Graewert, Dmitri Svergun, Gerald Gellermann, Christof Finkler, Jan O. Stracke, Atanas Koulov & Volker Schnaible - Antigen-Specific Antibody Glycosylation Is Regulated via Vaccination.
2016-03-16
Mahan AE, Jennewein MF, Suscovich T, Dionne K, Tedesco J, Chung AW, Streeck H, Pau M, Schuitemaker H, Francis D, Fast P, Laufer D, Walker BD, Baden L, Barouch DH, Alter G. - Glycation of polyclonal IgGs: Effect of sugar excipients during stability studies
2016-03-15
Leblanc Y, Bihoreau N, Jube M, Andre MH, Tellier Z, Chevreux G - Structural characterization of antibody drug conjugate by a combination of intact, middle-up and bottom-up techniques using sheathless capillary electrophoresis – tandem mass spectrometry as nanoESI infusion platform and separation method
2016-03-06
Nassur Said, Rabah Gahoual, Lauriane Kuhn, Alain Beck, Yannis-Nicolas François, Emmanuelle Leize-Wagner - Characterization of Therapeutic Monoclonal Antibodies at the Subunit-Level using Middle-Down 193 nm Ultraviolet Photodissociation
2016-03-05
Victoria C. Cotham and Jennifer S. Brodbelt - Antibody-based PET imaging of amyloid beta in mouse models of Alzheimer’s disease
2016-02-19
Dag Sehlin, Xiaotian T. Fang, Linda Cato, Gunnar Antoni, Lars Lannfelt & Stina Syvänen - Full validation of therapeutic antibody sequences by middle-up mass measurements and middle-down protein sequencing
2016-01-13
Resemann A, Jabs W, Wiechmann A, Wagner E, Colas O, Evers W, Belau E, Vorwerg L, Evans C, Beck A, Suckau D - Characterization of Cetuximab Fc/2 subunits aggregates by off-line CZE-MS
2016-01-05
Yannis-Nicolas François, Michael Biacchi, Nassur Said, Charly Renard, Alain Beck, Rabah Gahoual, Emmanuelle Leize-Wagner - Intact mass analysis of monoclonal antibodies by capillary electrophoresis—Mass spectrometry
2015-12-20
Mei Han, Brooke M. Rock, Josh T. Pearson, Dan A. Rock - N-glycan composition profiling for quality testing of biotherapeutics
2015-12-01
Christopher Taron, Rebecca Duke - Comprehensive glycosylation profiling of IgG and IgG-fusion proteins by top-down MS with multiple fragmentation techniques
2015-10-15
Bao Quoc Tran, Christopher Barton, Jinhua Feng, Aimee Sandjong, Sung Hwan Yoon, Shivangi Awasthi, Tao Liang, Mohd M. Khan, David P.A. Kilgour, David R. Goodlett, Young Ah Goo - Determination of the NISTmAb Primary Structure
2015-10-15
Trina Formolo, Mellisa Ly, Michaella Levy, Lisa Kilpatrick, Scott Lute, Karen Phinney, Lisa Marzilli, Kurt Brorson, Michael Boyne, Darryl Davis, John Schiel - Pan-ebolavirus and pan-filovirus mouse monoclonal antibodies: protection against Ebola and Sudan viruses.
2015-10-14
Frederick W. Holtsberg, Sergey Shulenin, Hong Vu, Katie A. Howell, Sonal J. Patel, Bronwyn Gunn, Marcus Karim, Jonathan R. Lai, Julia C. Frei, Elisabeth K. Nyakatura, Larry Zeitlin, Robin Douglas, Marnie L. Fusco, Jeffrey W. Froude, Erica Ollmann Saphire, Andrew S. Herbert, Ariel S. Wirchnianski, Calli M. Lear-Rooney, Galit Alter, John M. Dye, Pamela J. Glass, Kelly L. Warfield & M. Javad Aman - Orthogonal Methods for Characterizing the Unfolding of Therapeutic Monoclonal Antibodies: Differential Scanning Calorimetry, Isothermal Chemical Denaturation, and Intrinsic Fluorescence with Concomitant Static Light Scattering
2015-10-09
Deniz B. Temel, Pavel Landsman, Mark L. Brader - Characterization of monoclonal antibodies by a fast and easy LC- MS ToF analysis on culture supernatant
2015-08-05
Antoine Henninot, Aurelie Terrier, Julie Charton, Rémi Urbain, Alexandre Fontayne, Benoit Deprez, Terence Beghyn - Characterization of the Acidic Species of a Monoclonal Antibody Using Weak Cation Exchange Chromatography and LC-MS
2015-07-29
Gomathinayagam Ponniah, Adriana Kita, Christine Nowak, Alyssa Neill, Yekaterina Kori, Saravanamoorthy Rajendran, Hongcheng Liu - Real-Time Product Attribute Control to Manufacture Antibodies with Defined N-Linked Glycan Levels
2015-07-14
Craig Zupke, Lowell J. Brady, Peter G. Slade, Philip Clark, R. Guy Caspary, Brittney Livingston, Lisa Taylor, Kyle Bigham, Arvia E. Morris, Robert W. Bailey - Direct Identification of Rituximab Main Isoforms and Subunit Analysis by Online Selective Comprehensive Two-Dimensional Liquid Chromatography−Mass Spectrometry
2015-07-06
Dwight R. Stoll, David C. Harmes, John Danforth, Elsa Wagner, Davy Guillarme, Szabolcs Fekete, Alain Beck - Analytical characterization of IgG Fc subclass variants through high-resolution separation combined with multiple LC-MS identification
2015-06-16
Xiaomei (Annie) He, Nathaniel Washburn, Enrique Arevalo, John H. Robblee - Design and Pharmacokinetic Characterization of Novel Antibody Formats for Ocular Therapeutics
2015-06-12
Kapil Gadkar, Cinthia V. Pastuskovas, Jennifer E. Le Couter, J. Michael Elliott, Jianhuan Zhang, Chingwei V. Lee, Sarah Sanowar, Germaine Fuh, Hok Seon Kim, T. Noelle Lombana, Christoph Spiess, Makia Nakamura, Phil Hass, Whitney Shatz, Y. Gloria Meng, Justin M. Scheer - Microscale purification of antigen-specific antibodies
2015-06-08
Eric P. Brown, Erica Normandin, Nana Yaw Osei-Owusu, Alison E. Mahan, Ying N. Chan, Jennifer I. Lai, Monica Vaccari, Mangala Rao, Genoveffa Franchini, Galit Alter, Margaret E. Ackerman - A chemical and computational approach to comprehensive glycation characterization on antibodies
2015-06-01
Ramsey A Saleem, Brittany R Affholter, Sihong Deng, Phil C Campbell, Kelli Matthies, Catherine M Eakin & Alison Wallace - A Rapid Approach for Characterisation of Thiol-Conjugated ADCs and Calculation of Drug-Antibody Ratio by LC-MS
2015-06-01
David Firth, Leonard Bell, Martin Squires, Sian Estdale, Colin McKee - Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles-Part 2: mass spectrometric methods
2015-05-21
Dietmar Reusch, Markus Haberger, David Falck, Britta Peter, Bernd Maier, Jana Gassner, Michaela Hook, Katharina Wagner, Lea Bonnington, Patrick Bulau, Manfred Wuhrer - The Impact II, a Very High-Resolution Quadrupole Time-of-Flight Instrument (QTOF) for Deep Shotgun Proteomics
2015-05-19
Scarlet Beck, Annette Michalski, Oliver Raether, Markus Lubeck, Stephanie Kaspar, Niels Goedecke, Carsten Baessmann, Daniel Hornburg, Florian Meier, Igor Paron, Nils A. Kulak, Juergen Cox, and Matthias Mann - Production of α2,6-sialylated IgG1 in CHO cells
2015-04-01
Céline Raymondab, Anna Robothama, Maureen Spearmanc, Michael Butlerc, John Kellya, Yves Durocher - Characterization of the N‐Terminal Heterogeneities of Monoclonal Antibodies Using In-Gel Charge Derivatization of α-Amines and LC- MS/MS
2015-03-13
Daniel Ayoub, Diego Bertaccini, Heĺeǹe Diemer, Elsa Wagner-Rousset, Olivier Colas, Sarah Cianfeŕani, Alain Van Dorsselaer, Alain Beck, Christine Schaeffer-Reiss - Sialylated IgG-Fc: a novel biomarker of chronic inflammatory demyelinating polyneuropathy
2015-03-02
Anna Hiu Yi Wong, Yuki Fukami, Makoto Sudo, Norito Kokubun, Shinsuke Hamada, Nobuhiro Yuki - Characterization of antibody drug conjugate positional isomers at cysteine residues by peptide mapping LC–MS analysis
2015-02-15
Marie-Claire Janin-Bussat, Marina Dillenbourg, Nathalie Corvaia, Alain Beck, Christine Klinguer-Hamour - A method for high-throughput, sensitive analysis of IgG Fc and Fab glycosylation by capillary electrophoresis
2015-02-10
Alison E. Mahan, Jacquelynne Tedesco, Kendall Dionne, Kavitha Baruah, Hao D. Cheng, Philip L. De Jager, Dan H. Barouch, Todd Suscovicha, Margaret Ackermanc, Max Cripsin, Galit Alter - Investigation of Antibody Disulfide Reduction and Re-oxidation and Impact to Biological Activities
2015-02-05
Tian Wang Yaoqing Diana Liu Bing Cai Gang Huang Gregory C. Flynn - Monocyte Recruitment by HLA IgG-Activated Endothelium: The Relationship Between IgG Subclass and FcγRIIa Polymorphisms
2015-02-03
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2015-02-02
Alain Beck, François Debaene, Hélène Diemer, Elsa Wagner-Rousset, Olivier Colas, Alain Van Dorsselaer and Sarah Cianférani - Extensive glycosylation of ACPA-IgG variable domains modulates binding to citrullinated antigens in rheumatoid arthritis
2014-12-16
Yoann Rombouts, Annemiek Willemze, Joyce J B C van Beers, Jing Shi, Priscilla F Kerkman, Linda van Toorn, George M C Janssen, Arnaud Zaldumbide, Rob C Hoeben, Ger J M Pruijn, André M Deelder, Gertjan Wolbink, Theo Rispens, Peter A van Veelen, Tom W J Huizinga, Manfred Wuhrer, Leendert A Trouw, Hans U Scherer, René E M Toes - Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to Fcγ receptors
2014-12-15
Jérémy Loyaua, Pauline Malingea, Bruno Daubeufa, Limin Shanga, Greg Elsonab, Marie Kosco-Vilboisa, Nicolas Fischera & François Rousseaua - Structure and specificity of an antibody targeting a proteolytically cleaved IgG hinge
2014-08-01
Malia TJ, Teplyakov A, Brezski RJ, Luo J, Kinder M, Sweet RW, Almagro JC, Jordan RE, Gilliland GL - IgG Fab glycosylation analysis using a new mass spectrometric high-throughput profiling method reveals pregnancy-associated changes
2014-07-08
Bondt A, Rombouts Y, Selman MH, Hensbergen PJ, Reiding KR, Hazes JM, Dolhain RJ, Wuhrer M. - An EGFR Targeted PET Imaging Probe for the Detection of Colonic Adenocarcinomas in the Setting of Colitis
2014-07-01
Turker NS, Heidari P, Kucherlapati R, Kucherlapati M, Mahmood U - LC-MS analysis of polyclonal IgGs using IdeS enzymatic proteolysis for oxidation monitoring
2014-05-04
Leblanc Y, Romanin M, Bihoreau N, Chevreux G. - Double Blockade of CD14 and Complement C5 Abolishes the Cytokine Storm and Improves Morbidity and Survival in Polymicrobial Sepsis in Mice
2014-04-30
Huber-Lang M, Barratt-Due A, Pischke SE, Sandanger O, Nilsson PH, Nunn MA, Denk S, Gaus W, Espevik T, Mollnes TE. - Assessing analytical methods to monitor isoAsp formation in monoclonal antibodies
2014-04-29
Eakin CM, Miller A, Kerr J, Kung J, Wallace A. - A new tool for monoclonal antibody analysis Application of IdeS proteolysis in IgG domain-specific characterization
2014-04-07
Yan An, Ying Zhang, Hans-Martin Mueller, Mohammed Shameem, Xiaoyu Chen - From High- to Super-resolution Mass Spectrometry
2014-03-01
Tsybin YO - Middle-Down Analysis of Monoclonal Antibodies with Electron Transfer Dissociation Orbitrap Fourier Transform Mass Spectrometry
2014-02-18
Luca Fornelli, Daniel Ayoub, Konstantin Aizikov, Alain Beck, and Yury O. Tsybin - N- and O‑Glycosylation Analysis of Etanercept Using Liquid Chromatography and Quadrupole Time-of-Flight Mass Spectrometry Equipped with Electron-Transfer Dissociation Functionality
2013-12-05
Stephane Houel, Mark Hilliard, Ying Qing Yu, Niaobh McLoughlin, Silvia Millan Martin, Pauline M. Rudd, Jonathan P. Williams, and Weibin Chen - Antibody Drug Conjugate model fast characterization by LC-MS following IdeS proteolytic digestion.
2013-10-31
Wagner-Rousset E, Janin-Bussat MC, Colas O, Excoffier M, Haeuw JF, Rilatt I, Perez M, Corvaïa N, Beck A. - Rapid Fc glycosylation analysis of Fc fusions with IdeS and liquid chromatography mass spectrometry
2013-09-30
Lynaugh H, Li H, Gong B - Correct primary structure assessment and extensive glyco-profiling of cetuximab by a combination of intact, middle-up, middle-down and bottom-up ESI and MALDI mass spectrometry techniques.
2013-09-04
Ayoub D, Jabs W, Resemann A, Evers W, Evans C, Main L, Baessmann C, Wagner E, Suckau D, Beck A. - Engineered protease-resistant antibodies with selectable cell-killing functions
2013-09-01
Kinder M, Greenplate AR, Grugan KD, Soring KL, Heeringa KA, McCarthy SG, Bannish G, Perpetua M, Lynch F, Jordan RE, Strohl WR, Brezski RJ - Profiling formulated monoclonal antibodies by (1)H NMR spectroscopy
2013-09-01
Poppe L, Jordan JB, Lawson K, Jerums M, Apostol I, Schnier PD. - Formulation Development for Antibody-Drug Conjugates
2013-07-01
Junyan A. Ji, Jun Liu and Y. John Wang - Nondenaturing Size-Exclusion Chromatography-Mass Spectrometry to Measure Stress-Induced Aggregation in a Complex Mixture of Monoclonal Antibodies
2013-06-20
Woodard J, Lau H, Latypov RF - Analytical characterization of biosimilar antibodies and Fc- fusion proteins
2013-05-31
Beck A, Diemer H, Ayoub D, Debaene F, Wagner Rousset E, Carapito C, Dorsselaer Van A, Sanglier Cinférani S - Structural comparison of two anti-CD20 monoclonal antibody drug products using middle-down mass spectrometry
2013-04-12
Wang B, Gucinski AC, Keire DA, Buhse LF, Boyne Ii MT - Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry.
2013-03-15
Janin-Bussat MC, Tonini L, Huillet C, Colas O, Klinguer-Hamour C, Corvaïa N, Beck A. - Engineering and Characterization of the Chimeric Antibody that Targets the C-terminal Telopeptide of the α2 Chain of Human Collagen I: A Next Step in the Quest to Reduce Localized Fibrosis.
2013-02-22
Fertala J, Steplewski A, Kostas J, Beredjiklian P, Williams G, Arnold W, Abboud J, Bhardwaj A, Hou C, Fertala A. - Fluorogenic Tagging Methodology Applied to Characterize Oxidized Tyrosine and Phenylalanine in an Immunoglobulin Monoclonal Antibody
2013-02-15
Shuxia Zhou, Olivier Mozziconacci, Bruce A. Kerwin & Christian Schöneich - Protein digestion: An overview of the available techniques and recent developments
2013-01-31
Linda Switzar, Martin Giera, and Wilfried M.A. Niessen - Glycoproteomic analysis of antibodies
2013-01-16
Gerhild Zauner, Maurice H. J. Selman, Albert Bondt, Yoann Rombouts, Dennis Blank, André M. Deelder, Manfred Wuhrer - Structural analysis of a therapeutic monoclonal antibody dimer by hydroxyl radical footprinting.
2013-01-03
Deperalta G, Alvarez M, Bechtel C, Dong K, McRonald R, Ling V - Characterization of Fc-Fusion Protein Aggregates Derived from Extracellular Domain Disulfide Bond Rearrangements
2012-12-14
James Strand, Chi-Ting Huang, Jon Xu - Characterization of therapeutic antibodies and related products
2012-11-07
Alain Beck , Elsa Wagner-Rousset , Daniel Ayoub , Alain Van Dorsselaer , and Sarah Sanglier-Cianférani - Proteomics-directed cloning of circulating antiviral human monoclonal antibodies
2012-11-01
Sato S, Beausoleil SA, Popova L, Beaudet JG, Ramenani RK, Zhang X, Wieler JS, Schieferl SM, Cheung WC, Polakiewicz RD - Characterization of critical reagents in ligand-binding assays: enabling robust bioanalytical methods and lifecycle management
2012-11-01
Geist BJ, Egan AC, Yang TY, Dong Y, Shankar G. - Comparative Binding of Disulfide-Bridged PEG-Fabs.
2012-10-18
Khalili H, Godwin A, Choi JW, Lever R, Brocchini S - Fc Gamma Receptor CD64 Modulates the Inhibitory Activity of Infliximab
2012-08-24
Kacper A. Wojtal1*, Gerhard Rogler1,2, Michael Scharl1, Luc Biedermann1, Pascal Frei, Michael Fried, Achim Weber, Jyrki J. Eloranta, Gerd A. Kullak-Ublick, Stephan R. Vavricka - Determination of antibody glycosylation by mass spectrometry.
2012-06-27
Jäger C, Ferrara C, Umaña P, Zeck A, Regula JT, Koll H - Quantitative glycan profiling of normal human plasma derived immunoglobulin and its fragments Fab and Fc.
2012-06-06
Anumula KR - Analysis and functional consequences of increased Fab-sialylation of intravenous immunoglobulin (IVIG) after lectin fractionation.
2012-06-04
Käsermann F, Boerema DJ, Rüegsegger M, Hofmann A, Wymann S, Zuercher AW, Miescher S - Sialylation-independent mechanism involved in the amelioration of murine immune thrombocytopenia using intravenous gammaglobulin
2012-01-18
Danila Leontyev, Yulia Katsman, Xue-Zhong Ma, Sylvia Miescher, Fabian Käsermann and Donald R. Branc - The in vitro resistance of IgG2 to proteolytic attack concurs with a comparative paucity of autoantibodies against peptide analogs of the IgG2 hinge.
2011-11-01
Brezski RJ, Oberholtzer A, Strake B, Jordan RE - Rapid LC-MS screening for IgG Fc modifications and allelic variants in blood
2011-09-25
Andrew M. Goetzea, Zhongqi Zhanga, Ling Liub, Frederick W. Jacobsenb, Gregory C. Flynn - Rates and impact of human antibody glycation in vivo.
2011-09-19
Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC - Mechanism of immunoglobulin G4 Fab-arm exchange.
2011-06-15
Rispens T, Ooijevaar-de Heer P, Bende O, Aalberse RC - Fast analysis of recombinant monoclonal antibodies using IdeS proteolytic digestion and electrospray mass spectrometry.
2011-04-27
Chevreux G, Tilly N, Bihoreau N - Conjugation reactions in the preparations of quantum dot-based immunoluminescent probes for analysis of proteins by capillary electrophoresis.
2011-02-06
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2007-05-05
Hess JL, Porsch EA, Shertz CA, Boyle MD
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2022-09-23
Jui-Lin Chen, Chelsea N. Fries, Stella J. Berendam, Nicole S. Rodgers, Emily F. Roe, Yaoying Wu, Shuk Hang Li, Rishabh Jain, Brian Watts, Joshua Eudailey, Richard Barfield, Cliburn Chan , M. Anthony Moody , Kevin O. Saunders , Justin Pollara , Sallie R. Permar , Joel H. Collier, Genevieve G. Fouda - Proteolytic Profiling of Streptococcal Pyrogenic Exotoxin B (SpeB) by Complementary HPLC-MS Approaches
2021-12-30
Constantin Blöchl, Ulrich Eckhard, Christoph Holzner, Michela Luciano, Renate Bauer, Jutta Horejs-Hoeck, Hans Brandstetter and Christian G. Huber - Towards middle‑up analysis of polyclonal antibodies: subclass‑specific N‑glycosylation profiling of murine immunoglobulin G (IgG) by means of HPLC‑MS
2020-10-22
Constantin Blöchl, Christof Regl, Christian G. Huber, Petra Winter, Richard Weiss, Therese Wohlschlager - Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation
2020-08-25
Eveline A. N. Zeeuw van der Laan, Saskia van der Velden, Arthur E. H. Bentlage, Mads D. Larsen, Thijs L. J. van Osch, Juk Yee Mok, Giso Brasser, Dionne M. Geerdes, Carolien A. M. Koeleman, Jan Nouta, John W. Semple, Leendert Porcelijn, Wim J. E. van Esch, Manfred Wuhrer, C. Ellen van der Schoot, Gestur Vidarsson, Rick Kapur - Characterization of Whole and Fragmented Wild-Type Porcine IgG
2019-01-08
Claudia Nelson, Raymond Bacala, Baylie Gigolyk, Evelyn Ang, Haley Neustaeter, Emy Komatsu, Oleg Krokhin, Dave Hatcher and Hélène Perreault - A three-point identity criteria tool for establishing product identity using icIEF method
2018-03-01
Deepti Ahluwalia⁎, Madesh Belakavadi, Tapan K. Das, Amit Katiyar - SpeB Proteolysis with Imaged Capillary Isoelectric Focusing for the Characterization of Domain-Specific Charge Heterogeneities of Reference and Biosimilar Rituximab
2016-03-21
Zichuan Zhang, Ronel Perrault, Yun Zhao, Julia Ding - A method for high-throughput, sensitive analysis of IgG Fc and Fab glycosylation by capillary electrophoresis
2015-02-10
Alison E. Mahan, Jacquelynne Tedesco, Kendall Dionne, Kavitha Baruah, Hao D. Cheng, Philip L. De Jager, Dan H. Barouch, Todd Suscovicha, Margaret Ackermanc, Max Cripsin, Galit Alter
- Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells
2021-04-19
Christoph Gstöttner, Tao Zhang, Anja Resemann, Sophia Ruben, Stuart Pengelley, Detlev Suckau, Tim Welsink, Manfred Wuhrer and Elena Domínguez-Vega
- Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells
2021-04-19
Christoph Gstöttner, Tao Zhang, Anja Resemann, Sophia Ruben, Stuart Pengelley, Detlev Suckau, Tim Welsink, Manfred Wuhrer and Elena Domínguez-Vega
- Side-by-Side Comparison of uPAR-Targeting Optical Imaging Antibodies and Antibody Fragments for Fluorescence-Guided Surgery of Solid Tumors
2021-10-12
Victor M. Baart, Labrinus van Manen, Shadhvi S. Bhairosingh, Floris A. Vuijk, Luisa Iamele, Hugo de Jonge, Claudia Scotti, Massimo Resnati, Robert A. Cordfunke, Peter J. K. Kuppen, Andrew P. Mazar, Jacobus Burggraaf, Alexander L. Vahrmeijer & Cornelis F. M. Sier - Application of middle-down approach in quantitation and catabolite identification of protein by LC–high-resolution mass spectrometry
2021-03-15
Lijuan Kang, Shengsheng Xu, Yongle Pang, Tom Kirchner, Yue-mei Zhang, Wilson Edwards, Raul Camacho, Lisa Norquay, Naidong Weng & Wenying Jian - Using different proteolytic enzymes to digest antibody and its impact on stability of antibody mimetics
2020-11-21
Hanieh Khalili - Protein-Functionalized Gold Nanoparticles as Refractometric Nanoplasmonic Sensor for Detection of Proteolytic Activity of Porphyromonas gingivalis
2020-09-16
Anna Svärd, Jessica Neilands, Eleonor Palm, Gunnel Svensäter, Torbjörn Bengtsson, and Daniel Aili - Comparing different domains of analysis for the characterisation of N-Glycans on monoclonal antibodies
2019-11-29
Sara Carillo, Raquel Pérez-Robles, CraigJakes, Meire Ribeiro da Silva, Silvia Millán Martín, Amy Farrell, Natalia Navas, Jonathan Bones - Susceptibility of protein therapeutics to spontaneous chemical modifications by oxidation, cyclization, and elimination reactions
2019-10-01
Luigi Grassi, Chiara Cabrele - Middle-Down Multi-Attribute Analysis of Antibody- Drug Conjugates with Electron Transfer Dissociation
2019-08-23
Bifan Chen, Ziqing Lin, Yanlong Zhu, Yutong Jin, Eli Larson, Qingge Xu, Cexiong Fu, Zhaorui Zhang, Qunying Zhang, Wayne Pritts, and Ying Ge - Structural Analysis of Monoclonal Antibodies by Ultrahigh Resolution MALDI In-Source Decay FT-ICR Mass Spectrometry
2018-12-20
Yuri E.M. van der Burgt, David P. A. Kilgour, Yury O. Tsybin, Kristina Srzentic,́ Luca Fornelli, Alain Beck, Manfred Wuhrer, and Simone Nicolardi - Multiplexed Middle-Down Mass Spectrometry Reveals Light and Heavy Chain Connectivity in a Monoclonal Antibody
2018-09-25
Kristina Srzentic, Konstantin O. Nagornov, Luca Fornelli, Anna A. Lobas, Daniel Ayoub, Anton Kozhinov, Natalia Gasilova, Laure Menin, Alain Beck, Mikhail Gorshkov, Konstantin Aizikov, and Yury O. Tsybin - Accurate Sequence Analysis of a Monoclonal Antibody by Top- Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques
2018-06-12
Luca Fornelli, Kristina Srzentić, Romain Huguet, Christopher Mullen, Seema Sharma, Vlad Zabrouskov, Ryan T. Fellers, Kenneth R. Durbin, Philip D. Compton, Neil L. Kelleher - Cysteine-SILAC Mass Spectrometry Enabling the Identification and Quantitation of Scrambled Interchain Disulfide Bonds: Preservation of Native Heavy-Light Chain Pairing in Bispecific IgGs Generated by Controlled Fab-arm Exchange
2017-09-18
Ewald T. J. van den Bremer, Aran F. Labrijn, Ramon van den Boogaard, Patrick Priem, Kai Scheffler, Joost P. M. Melis, Janine Schuurman, Paul W. H. I. Parren and Rob N. de Jong - GingisKHAN™ protease cleavage allows a high-throughput antibody to Fab conversion enabling direct functional assessment during lead identification of human monoclonal and bispecific IgG1 antibodies
2017-08-14
Moelleken J, Endesfelder M, Gassner C, Lingke S, Tomaschek S, Tyshchuk O, Lorenz S, Reiff U, Mølhøj M. - Charge variants characterization of a monoclonal antibody by ion exchange chromatography coupled on-line to native mass spectrometry: Case study after a long-term storage at +5 ◦C
2017-02-14
Y. Leblanc, C. Ramon, N. Bihoreau, G. Chevreux
- Histone Sample Preparation for Bottom-Up Mass Spectrometry: A Roadmap to Informed Decisions
2021-04-21
Simon Daled, Sander Willems, Bart Van Puyvelde, Laura Corveleyn, Sigrid Verhelst, Laura De Clerck, Dieter Deforce and Maarten Dhaenens - Protein-Functionalized Gold Nanoparticles as Refractometric Nanoplasmonic Sensor for Detection of Proteolytic Activity of Porphyromonas gingivalis
2020-09-16
Anna Svärd, Jessica Neilands, Eleonor Palm, Gunnel Svensäter, Torbjörn Bengtsson, and Daniel Aili - Probing protein conformation destabilization in sterile liquid formulations through the formation of 3,4-dihydroxyphenylalanine
2020-09-10
Olivier Mozziconacci, Natalia Subelzu, Christian Schoneich, Yong Liu, Andreas Abend, and W Peter Wuelfing - Comprehensive histone epigenetics: A mass spectrometry based screening assay to measure epigenetic toxicity
2020-09-01
Sigrid Verhelst, Laura De Clerck, Sander Willems, Bart Van Puyvelde, Simon Daled, Dieter Deforce, Maarten Dhaenens - Detecting molecular forms of antithrombin by LC-MRM-MS: defining the measurands
2018-05-01
L. Renee Ruhaak, Fred P.H.T.M. Romijn, Nico P.M. Smit, Arnoud van der Laarse, Mervin M. Pieterse, Moniek P.M. de Maat, Fred J.L.M. Haas, Cornelis Kluft, Jean Amiral, Piet Meijer and Christa M. Cobbaert
- A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans
2022-10-24
Thapakorn Jaroentomeechai, Yong Hyun Kwon, Yiwen Liu, Olivia Young, Ruchika Bhawal, Joshua D. Wilson, Mingji Li, Digantkumar G. Chapla, Kelley W. Moremen, Michael C. Jewett, Dario Mizrachi & Matthew P. DeLisa - EGFR binding Fc domain-drug conjugates: stable and highly potent cytotoxic molecules mediate selective cell killing
2021-09-20
Sebastian Jäger, Stephan Dickgiesser, Jason Tonillo, Stefan Hecht, Harald Kolmar and Christian Schröter - Site-specific glycan-conjugated NISTmAb antibody drug conjugate mimetics: synthesis, characterization, and utility
2021-07-06
Brian Agnew, Shanhua Lin, Terry Zhang, Robert Aggeler, Trina Mouchahoir & John Schiel - Siplizumab Induces NK Cell Fratricide Through Antibody-Dependent Cell-Mediated Cytotoxicity
2021-02-11
Christian Binder, Felix Sellberg, Filip Cvetkovski, Stefan Berg, Erik Berglund and David Berglund - High microbiota reactivity of adult human intestinal IgA requires somatic mutations
2020-07-08
Johanna Kabbert, Julia Benckert, Tim Rollenske, Thomas C.A. Hitch, Thomas Clavel, Vuk Cerovic, Hedda Wardemann, Oliver Pabst - Glycan-mediated technology for obtaining homogeneous site-specific conjugated antibody-drug conjugates: synthesis and analytical characterization by using complementary middle-up LC/HRMS analysis
2020-05-14
Bastiaan L. Duivelshof, Evolène Deslignière, Oscar Hernandez-Alba, Anthony Ehkirch, Hanna Toftevall, Jonathan Sjögren, Sarah Cianférani, Alain Beck, Davy Guillarme, and Valentina D'Atri - Antibody transcytosis across brain endothelial-like cells occurs nonspecifically and independent of FcRn
2020-02-28
John S. Ruano-Salguero, Kelvin H. Lee - On enzymatic remodeling of IgG glycosylation; unique tools with broad applications
2019-10-17
Jonathan Sjögren, Rolf Lood, Andreas Nägeli - Middle-Down Multi-Attribute Analysis of Antibody- Drug Conjugates with Electron Transfer Dissociation
2019-08-23
Bifan Chen, Ziqing Lin, Yanlong Zhu, Yutong Jin, Eli Larson, Qingge Xu, Cexiong Fu, Zhaorui Zhang, Qunying Zhang, Wayne Pritts, and Ying Ge - Molecular Basis of Broad Spectrum N-Glycan Specificity and Processing of Therapeutic IgG Monoclonal Antibodies by Endoglycosidase S2
2019-02-06
Erik H. Klontz, Beatriz Trastoy, Daniel Deredge, James K. Fields, Chao Li, Jared Orwenyo, Alberto Marina, Robert Beadenkop, Sebastian Günther, Jair Flores, Patrick L. Wintrod, Lai-Xi Wang, Marcelo E. Guerin, and Eric J. Sundberg - Protocols for the analytical characterization of therapeutic monoclonal antibodies. II – Enzymatic and chemical sample preparation
2017-06-19
Balazs Bobaly, Valentina D’Atri, Alexandre Goyon, Olivier Colas, Alain Beck, Szabolcs Fekete, Davy Guillarme - Monitoring Glycosylation Profile and Protein Titer in Cell Culture Samples Using ZipChip CE-MS
2017-04-08
Yan Wang, Peng Feng, Zoran Sosic and Li Zang - Orthogonal liquid chromatography–mass spectrometry methods for the comprehensive characterization of therapeutic glycoproteins, from released glycans to intact protein level
2017-03-27
Eric Largy, Fabrice Cantais, Géry Van Vyncht, Alain Beck, Arnaud Delobel - Orthogonal Assessment of Biotherapeutic Glycosylation: A Case Study Correlating N-Glycan Core Afucosylation of Herceptin with Mechanism of Action
2016-09-12
Rosie Upton, Leonard Bell, Colin Guy, Paul Caldwell, Sian Estdale, Perdita E. Barran and David Firth - Rapid quantitation of monoclonal antibody N-glyco-occupancy and afucosylation using mass spectrometry.
2016-07-01
Suli Liu, Li Zang - Potential of Hydrophilic Interaction Chromatography for the Analytical Characterization of Protein Biopharmaceuticals
2016-04-19
Aur´elie Periat Szabolcs Fekete Alessandra Cusumano Jean-Luc Veuthey Alain Beck Matthew Lauber Davy Guillarme - N-glycan composition profiling for quality testing of biotherapeutics
2015-12-01
Christopher Taron, Rebecca Duke - Evaluation of a glycoengineered monoclonal antibody via LC-MS analysis in combination with multiple enzymatic digestion
2015-10-29
Renpeng Liu, John Giddens, Colleen M. McClung, Paula E. Magnelli, Lai-Xi Wang & Ellen P. Guthrie - EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans
2015-07-08
Jonathan Sjögren, Eoin F J Cosgrave, Maria Allhorn, Maria Nordgren, Stephan Björk, Fredrik Olsson, Sarah Fredriksson, Mattias Collin
- State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates Synthesis
2021-05-24
Evolène Deslignière, Anthony Ehkirch, Bastiaan L. Duivelshof, Hanna Toftevall, Jonathan Sjögren, Davy Guillarme, Valentina D’Atri, Alain Beck, Oscar Hernandez-Alba, and Sarah Cianférani - MINSTED fluorescence localization and nanoscopy
2021-04-20
Michael Weber, Marcel Leutenegger, Stefan Stoldt, Stefan Jakobs, Tiberiu S. Mihaila, Alexey N. Butkevich & Stefan W. Hell - Photoactivatable Fluorophore for Stimulated Emission Depletion(STED) Microscopy and Bioconjugation Technique forHydrophobic Labels
2020-12-04
Michael Weber, Taukeer A. Khan, Lukas J. Patalag, Mariano Bossi, Marcel Leutenegger, Vladimir N. Belov and Stefan W. Hell - Glycan-mediated technology for obtaining homogeneous site-specific conjugated antibody-drug conjugates: synthesis and analytical characterization by using complementary middle-up LC/HRMS analysis
2020-05-14
Bastiaan L. Duivelshof, Evolène Deslignière, Oscar Hernandez-Alba, Anthony Ehkirch, Hanna Toftevall, Jonathan Sjögren, Sarah Cianférani, Alain Beck, Davy Guillarme, and Valentina D'Atri - Quantitative PET imaging of PD-L1 expression in xenograft and syngeneic tumour models using a site-specifically labelled PD-L1 antibody
2019-12-27
Camilla Christensen, Lotte K. Kristensen, Maria Z. Alfsen, Carsten H. Nielsen, Andreas Kjaer - Site-specifically labeled 89Zr-DFO-trastuzumab improves immuno-reactivity and tumor uptake for immuno-PET in a subcutaneous HER2-positive xenograft mouse model
2019-06-09
Lotte K. Kristensen, Camilla Christensen, Mette M. Jensen, Brian J. Agnew, Christina Schjöth-Frydendahl, Andreas Kjaer, Carsten H. Nielsen
- State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates Synthesis
2021-05-24
Evolène Deslignière, Anthony Ehkirch, Bastiaan L. Duivelshof, Hanna Toftevall, Jonathan Sjögren, Davy Guillarme, Valentina D’Atri, Alain Beck, Oscar Hernandez-Alba, and Sarah Cianférani - C-terminal lysine clipping of IgG1: impact on binding to human FcγRIIIa and neonatal Fc receptors
2021-01-18
Valegh Faid, Yann Leblanc, Marie Berger, Alexander Seifert, Nicolas Bihoreau, Guillaume Chevreux - In vitro-transcribed antigen receptor mRNA nanocarriers for transient expression in circulating T cells in vivo
2020-11-27
N. N. Parayath, S. B. Stephan, A. L. Koehne, P. S. Nelson & M. T. Stephan - Towards automation of Collision Induced Unfolding experiments through online Size Exclusion Chromatography coupled to native Mass Spectrometry
2020-09-04
Evolène Deslignière, Anthony Ehkirch, Thomas Botzanowski, Alain Beck, Oscar Hernandez-Alba, and Sarah Cianférani - Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation
2020-08-25
Eveline A. N. Zeeuw van der Laan, Saskia van der Velden, Arthur E. H. Bentlage, Mads D. Larsen, Thijs L. J. van Osch, Juk Yee Mok, Giso Brasser, Dionne M. Geerdes, Carolien A. M. Koeleman, Jan Nouta, John W. Semple, Leendert Porcelijn, Wim J. E. van Esch, Manfred Wuhrer, C. Ellen van der Schoot, Gestur Vidarsson, Rick Kapur - Inter-laboratory Study for Characterizing Monoclonal Antibodies by Top-Down and Middle-Down Mass Spectrometry
2020-07-31
Kristina Srzentic, Luca Fornelli, Yury O. Tsybin, Joseph A. Loo, Henrique Seckler, Jeffrey N. Agar, Lissa C. Anderson, Dina L. Bai, Alain Beck, Jennifer S. Brodbelt, Yuri E.M. van der Burgt, Julia Chamot-Rooke, Sneha Chatterjee, Yunqiu Chen, David James Clarke, Paul O. Danis, Jolene K. Diedrich, Robert Anthony D'Ippolito, Mathieu Dupré, Natalia Gasilova, Ying Ge, Young Ah Goo, David R. Goodlett, Sylvester M. Greer, Kim F. Haselmann, Lidong He, Christopher L. Hendrickson, Joshua D. Hinkle, Matthew Holt, Sam Hughes, Donald F. Hunt, Neil L Kelleher, Anton N. Kozhinov, Ziqing Lin, Christian Malosse, Alan G. Marshall, Laure Menin, Robert J. Millikin, Konstantin O. Nagornov, Simone Nicolardi, Ljiljana Paša-Tolić, Stuart Pengelley, Neil R. Quebbemann, Anja Resemann, Wendy Sandoval, Richa Sarin, Nicholas Daniel Schmitt, Jeffrey Shabanowitz, Jared B. Shaw, Michael R. Shortreed, Lloyd M. Smith, Frank Sobott, Detlev Suckau, Timothy Toby, Chad R. Weisbrod, Norelle C. Wildburger, John R. Yates, Sung Hwan Yoon, Nicolas L. Young, and Mowei Zhou - Middle Level IM–MS and CIU Experiments for Improved Therapeutic Immunoglobulin Subclass Fingerprinting
2020-05-26
Thomas Botzanowski, Oscar Hernandez-Alba, Martine Malissard, Elsa Wagner-Rousset, Evolène Deslignière, Olivier Colas, Jean-François Haeuw, Alain Beck, and Sarah Cianférani - Comparison of two glycoengineering strategies to control the fucosylation of a monoclonal antibody
2020-02-20
Neha Mishraa, Maureen Spearman, Lynda Donald, Helene Perreault, Michael Butler - Implementation of a High-Resolution Liquid Chromatography–Mass Spectrometry Method in Quality Control Laboratories for Release and Stability Testing of a Commercial Antibody Product
2019-12-23
Izabela Sokolowska, Jingjie Mo,Fatie Rahimi Pirkolachahi, Carol McVean, Lars A. T. Meijer, Linda Switzar, Crina Balog, Michael J. Lewis, Ping Hu - Understanding how Fc-modification transforms a pathogenic HIT-like monoclonal antibody into a novel treatment for sepsis
2019-11-13
Kandace Gollomp, Amrita Sarkar, Sanjiv Harikumar, Steven H Seeholzer, Gowthami M. Arepally, Kristin Hudock, Lubica Rauova, M. Anna Kowalska, Mortimer Poncz - On enzymatic remodeling of IgG glycosylation; unique tools with broad applications
2019-10-17
Jonathan Sjögren, Rolf Lood, Andreas Nägeli - Two sequential layers of antibody‐mediated control of Legionella pneumophila infection
2019-05-10
Stefan S. Weber, Diana Stoycheva, Falk Nimmerjahn, Annette Oxenius - Sample Preparation for LC‐MS Bioanalysis of Antibody–Drug Conjugates
2019-02-25
Cong Wei Ragu Ramanathan - Fc Sialylation Prolongs Serum Half-Life of Therapeutic Antibodies
2019-01-25
Mathilde Bas, Aurélie Terrier, Emilie Jacque, Aurélie Dehenne, Virginie Pochet-Béghin, Cécile Beghin, Anne-Sophie Dezetter, Gilles Dupont, Anaïs Engrand, Benjamin Beaufils, Philippe Mondon, Nathalie Fournier, Christophe de Romeuf, Sylvie Jorieux, Alexandre Fontayne, Lennart T. Mars and Céline Monnet - Mechanical strain determines the site-specific localization of inflammation and tissue damage in arthritis
2018-11-05
Isabelle Cambré, Djoere Gaublomme, Arne Burssens, Peggy Jacques, Nadia Schryvers, Amélie De Muynck, Leander Meuris, Stijn Lambrecht, Shea Carter, Pieter de Bleser, Yvan Saeys, Luc Van Hoorebeke, George Kollias, Matthias Mack, Paul Simoens, Rik Lories, Nico Callewaert, Georg Schett & Dirk Elewaut - Comprehensive characterization of monoclonal antibody by Fourier transform ion cyclotron resonance mass spectrometry
2018-09-19
Yutong Jin, Ziqing Lin, Qingge Xu, Cexiong Fu, Zhaorui Zhang, Qunying Zhang, Wayne A. Pritts, Ying Ge - Variable domain N-linked glycosylation and negative surface charge are key features of monoclonal ACPA: implications for B-cell selection
2018-04-06
Katy A. Lloyd, Johanna Steen, Khaled Amara, Philip J. Titcombe, Lena Israelsson, Susanna L. Lundström, Diana Zhou, Roman A. Zubarev, Evan Reed, Luca Piccoli, Cem Gabay, Antonio Lanzavecchia, Dominique Baeten, Karin Lundberg, Daniel L. Mueller, Lars Klareskog, Vivianne Malmström, and Caroline Grönwall - Glyco-engineered Long Acting FGF21 Variant with Optimal Pharmaceutical and Pharmacokinetic Properties to Enable Weekly to Twice Monthly Subcutaneous Dosing
2018-03-09
Yan Weng, Tetsuya Ishino, Annette Sievers, Saswata Talukdar, Jeffrey R. Chabot, Amy Tam, Weili Duan, Kelvin Kerns, Eric Sousa, Tao He, Alison Logan, Darwin Lee, Dongmei Li, Yingjiang Zhou, Barbara Bernardo, Alison Joyce, Mania Kavosi, Denise M. O’Hara, Tracey Clark, Jie Guo, Craig Giragossian, Mark Stahl, Roberto A. Calle, Ron Kriz, Will Somers & Laura Lin - Quantitative analysis of glycation and its impact on antigen binding
2018-03-05
Jingjie Mo, Renzhe Jin, Qingrong Yan, Izabela Sokolowska, Michael J. Lewis & Ping Hu - Clinical assay for direct assessment of IgG galactosylation in serum using endoglycosidase S
2018-02-22
Dieter Vanderschaeghe, Leander Meuris, Hendrik Grootaert, Tom Raes, Annelies Van Hecke, Xavier Verhelst, Frederique Van de Velde, Bruno Lapauw, Hans Van Vlierberghe, and Nico Callewaert - Subunits analysis approach for the determination of fucosylation levels in monoclonal antibodies using LC-HRAM-MS
2018-01-01
Craig Jakes, Sara Carillo, Izabela Zaborowska, and Jonathan Bones National Institute for Bioprocessing Research and Training (NIBRT) - One-pot enzymatic glycan remodeling of a therapeutic monoclonal antibody by endoglycosidase S (Endo-S) from Streptococcus pyogenes
2017-07-29
XinTong, Tiezheng Li, Jared Orwenyo, Christian Toonstra, Lai-Xi Wang - Subunit mass analysis for monitoring antibody oxidation
2017-01-20
Izabela Sokolowska, Jingjie Mo, Jia Dong, Michael J. Lewis, Ping Hu - Calculated conjugated payload from immunoassay and LC-MS intact protein analysis measurements of antibody-drug conjugate
2016-09-29
B. Rago, T. Clark, L. King, J. Zhang, L. N. Tumey, F. Li, F. Barletta, C. Wei, M. Leal,S. Hansel & X. Han - Orthogonal Assessment of Biotherapeutic Glycosylation: A Case Study Correlating N-Glycan Core Afucosylation of Herceptin with Mechanism of Action
2016-09-12
Rosie Upton, Leonard Bell, Colin Guy, Paul Caldwell, Sian Estdale, Perdita E. Barran and David Firth - Unconjugated payload quantification and DAR characterization of antibody–drug conjugates using high-resolution MS
2016-08-03
Leanne Grafmuller, Cong Wei, Ragu Ramanathan, Frank Barletta, Rick Steenwyk & Joseph Tweed - High-Throughput, Automated Protein A Purification Platform with Multiattribute LC-MS Analysis for Advanced Cell Culture Process Monitoring
2016-08-03
Jia Dong, Nicole Migliore, Steven J. Mehrman, John Cunningham, Michael J. Lewis and Ping Hu - Glycation of polyclonal IgGs: Effect of sugar excipients during stability studies
2016-03-15
Leblanc Y, Bihoreau N, Jube M, Andre MH, Tellier Z, Chevreux G - Effect of high mannose glycan pairing on IgG antibody clearance.
2016-02-22
Liu YD, Flynn GC - N-glycan composition profiling for quality testing of biotherapeutics
2015-12-01
Christopher Taron, Rebecca Duke - Characterization of monoclonal antibodies by a fast and easy LC- MS ToF analysis on culture supernatant
2015-08-05
Antoine Henninot, Aurelie Terrier, Julie Charton, Rémi Urbain, Alexandre Fontayne, Benoit Deprez, Terence Beghyn - EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans
2015-07-08
Jonathan Sjögren, Eoin F J Cosgrave, Maria Allhorn, Maria Nordgren, Stephan Björk, Fredrik Olsson, Sarah Fredriksson, Mattias Collin - Native mass spectrometry and ion mobility characterization of trastuzumab emtansine, a lysine-linked antibody drug conjugat
2015-02-18
Julien Marcoux, Thierry Champion, Olivier Colas, Elsa Wagner-Rousset, Nathalie Corvaïa, Alain Van Dorsselaer, Alain Beck and Sarah Cianférani - Site-Specific Conjugation of Monomethyl Auristatin E to Anti-CD30 Antibodies Improves Their Pharmacokinetics and Therapeutic Index in Rodent Models
2015-02-09
F. Lhospice, D. Brégeon ,C. Belmant ,P. Dennler, A. Chiotellis, E. Fischer, L. Gauthier, A. Boëdec, H. Rispaud, S. Savard-Chambard, A. Represa, N. Schneider, C. Paturel, M. Sapet, C. Delcambre, S. Ingoure, N. Viaud , C. Bonnafous , R. Schibli , and F. Romagné - Cutting-edge mass spectrometry characterization of originator, biosimilar and biobetter antibodies
2015-02-02
Alain Beck, François Debaene, Hélène Diemer, Elsa Wagner-Rousset, Olivier Colas, Alain Van Dorsselaer and Sarah Cianférani - Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to Fcγ receptors
2014-12-15
Jérémy Loyaua, Pauline Malingea, Bruno Daubeufa, Limin Shanga, Greg Elsonab, Marie Kosco-Vilboisa, Nicolas Fischera & François Rousseaua - Adsorption and recovery issues of recombinant monoclonal antibodies in reversed-phase liquid chromatography
2014-10-22
Szabolcs FEKETE, Alain BECK, Elsa WAGNER, Karine VUIGNIER, Davy GUILLARME - Deglycosylation of mAb by EndoS for Improived Molecular Imaging
2014-08-19
Peng Gao, Kenneth L. Pinkston, Nathaniel Wilganowski, Holly Robinson, Ali Azhdarinia, Banghe Zhu, Eva M. Sevick-Muraca, Barrett R. Harvey - LC-MS analysis of polyclonal IgGs using IdeS enzymatic proteolysis for oxidation monitoring
2014-05-04
Leblanc Y, Romanin M, Bihoreau N, Chevreux G. - Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry.
2013-03-15
Janin-Bussat MC, Tonini L, Huillet C, Colas O, Klinguer-Hamour C, Corvaïa N, Beck A. - Characterization of therapeutic antibodies and related products
2012-11-07
Alain Beck , Elsa Wagner-Rousset , Daniel Ayoub , Alain Van Dorsselaer , and Sarah Sanglier-Cianférani - A Novel Assay for Antibody-Dependent Cell-Mediated Cytotoxicity against HIV-1- or SIV-Infected Cells Reveals Incomplete Overlap with Antibodies Measured by Neutralization and Binding Assays.
2012-08-29
Alpert MD, Heyer LN, Williams DE, Harvey JD, Greenough T, Allhorn M, Evans DT. - Fc Gamma Receptor CD64 Modulates the Inhibitory Activity of Infliximab
2012-08-24
Kacper A. Wojtal1*, Gerhard Rogler1,2, Michael Scharl1, Luc Biedermann1, Pascal Frei, Michael Fried, Achim Weber, Jyrki J. Eloranta, Gerd A. Kullak-Ublick, Stephan R. Vavricka - Quantitative glycan profiling of normal human plasma derived immunoglobulin and its fragments Fab and Fc.
2012-06-06
Anumula KR - Rapid LC-MS screening for IgG Fc modifications and allelic variants in blood
2011-09-25
Andrew M. Goetzea, Zhongqi Zhanga, Ling Liub, Frederick W. Jacobsenb, Gregory C. Flynn - Rates and impact of human antibody glycation in vivo.
2011-09-19
Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC
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Valentina Rangel-Angarita and Stacy A. Malaker - Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells
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2018-12-04
Valentina D’Atri, Lucie Novaḱova, Szabolcs Fekete, Dwight Stoll, Matthew Lauber, Alain Beck, and Davy Guillarme
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Kelly A. Dingess, Max Hoek, Danique M. H. van Rijswijk, Sem Tamara, Maurits A. den Boer, Tim Veth1,2, Mirjam J. A. Damen1,2, Arjan Barendregt1,2, Michelle Romijn, Hannah G. Juncker, Britt J. van Keulen, Gestur Vidarsson, Johannes B. van Goudoever, Albert Bondt and Albert J. R. Heck - Precise O-Glycosylation Site Localization of CD24Fc by LC-MS Workflows
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Xiaojuan Li, Robert Wilmanowski, Xinliu Gao, Zachary L. VanAernum, Daniel P. Donnelly, Brent Kochert, Hillary A. Schuessler, and Douglas Richardson - Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells
2021-04-19
Christoph Gstöttner, Tao Zhang, Anja Resemann, Sophia Ruben, Stuart Pengelley, Detlev Suckau, Tim Welsink, Manfred Wuhrer and Elena Domínguez-Vega - Generating Informative Sequence Tags from Antigen-Binding Regions of Heavily Glycosylated IgA1 Antibodies by Native Top-Down Electron Capture Dissociation
2021-02-11
Jean-Francois Greisch, Maurits A. den Boer, Frank Beurskens, Janine Schuurman, Sem Tamara, Albert Bondt, Albert J. R. Heck - Glycoproteomics: growing up fast
2020-12-07
Thomas. D & Scott. N - Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila
2020-09-24
Beatriz Trastoy, Andreas Naegeli, Itxaso Anso, Jonathan Sjögren, Marcelo E. Guerin - Large-scale site-specific mapping of the O-GalNAc glycoproteome
2020-07-17
Weiming Yang, Angellina Song, Minghui Ao, Yuanwei Xu & Hui Zhang - Comprehensive N- and O-glycosylation Mapping of Human Coagulation Factor V
2020-04-20
Cheng Ma, Ding Liu, Dong Li, Junping Zhang, Xiao‐Qian Xu, He Zhu, Xiu‐Feng Wan, Carol H. Miao, Barbara A. Konkle, Philip Onigman, Weidong Xiao, Lei Li - Advances toward mapping the full extent of protein site-specific O-GalNAc glycosylation that better reflects underlying glycomic complexity
2019-03-16
Kay-Hooi Khoo - Mapping the O‐glycoproteome using site‐specific extraction of O‐linked glycopeptides (EXoO)
2018-11-20
Weiming Yang, Minghui Ao, Yingwei Hu, Qing Kay Li, Hui Zhang - Deciphering Protein O‑Glycosylation: Solid-Phase Chemoenzymatic Cleavage and Enrichment
2018-06-24
Yang S., Onigman P., Wu WW., Sjogren J., Nyhlen H., Shen RF. and Cipollo J.
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2022-11-03
Y. Diana Liu , Lance Cadang, Karenna Bol, Xiao Pan, Katherine Tschudi, Mansour Jazayri, Julien Camperi , David Michels, John Stults, Reed J. Harris and Feng Yang - Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences
2022-04-09
Judy Shon, Daniel Fernandez, Nicholas M.Riley, Michael J.Ferracane, Carolyn R.Bertozzi - Glycoform analysis of intact erythropoietin by MALDI FT-ICR mass spectrometry
2021-09-21
Steffen Lippold, Raashina Thavarajah, Dietmar Reusch, Manfred Wuhrer, Simone Nicolardi - Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells
2021-04-19
Christoph Gstöttner, Tao Zhang, Anja Resemann, Sophia Ruben, Stuart Pengelley, Detlev Suckau, Tim Welsink, Manfred Wuhrer and Elena Domínguez-Vega - Generating Informative Sequence Tags from Antigen-Binding Regions of Heavily Glycosylated IgA1 Antibodies by Native Top-Down Electron Capture Dissociation
2021-02-11
Jean-Francois Greisch, Maurits A. den Boer, Frank Beurskens, Janine Schuurman, Sem Tamara, Albert Bondt, Albert J. R. Heck - Large-scale site-specific mapping of the O-GalNAc glycoproteome
2020-07-17
Weiming Yang, Angellina Song, Minghui Ao, Yuanwei Xu & Hui Zhang - Comprehensive N- and O-glycosylation Mapping of Human Coagulation Factor V
2020-04-20
Cheng Ma, Ding Liu, Dong Li, Junping Zhang, Xiao‐Qian Xu, He Zhu, Xiu‐Feng Wan, Carol H. Miao, Barbara A. Konkle, Philip Onigman, Weidong Xiao, Lei Li - Calcium-activated chloride channel regulator 1 (CLCA1) forms non-covalent oligomers in colonic mucus and has Mucin 2–processing properties
2019-09-29
Elisabeth E.L. Nyström, Liisa Arike, Erik Ehrencrona, Gunnar C. Hansson, and Malin E.V. Johansson - Orthogonal Middle-up Approaches for Characterization of the Glycan Heterogeneity of Etanercept by Hydrophilic Interaction Chromatography Coupled to High-Resolution Mass Spectrometry
2018-12-04
Valentina D’Atri, Lucie Novaḱova, Szabolcs Fekete, Dwight Stoll, Matthew Lauber, Alain Beck, and Davy Guillarme