Powered by Bioz

FabRICATOR Low Endotoxin is available as a lyophilized powder in two different sizes; 2000 and 5000 units, for digestion of 2 or 5 mg IgG, respectively. This product formulation has low levels of endotoxins per vial, with <0.04 EU /vial for the 2000 unit vials and <0.10 EU/vial for the 5000 unit vials.
 

• Efficient digestion with documented low levels of endotoxin
• Suitable for use in sensitive in vivo or cell-based assays

Unit Definition

One unit digests ≥ 95% of 1 µg human IgG when incubated in 10 mM sodium phosphate, 137 mM NaCl, 2.7 mM KCl pH 7.4 at 37°C for 30 min.

Storage and Stability

The FabRICATOR Low Endotoxin is reconstituted by addition of water. No preservatives added. After reconstitution, it is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.

Buffers

The buffers below have been tested for compatibility with FabRICATOR digestion at different pH. Note that the FabRICATOR enzyme is irreversibly inactivated below pH 5.0. Digestion above pH 8.0 requires prolonged/optimized conditions.

• Phosphate buffer saline (PBS) 6.0 – 8.0
• Tris buffer 7.0 – 8.0
• MES buffer 5.5 – 6.5
• HEPES buffer 7.0 – 8.0
• Ammonium bicarbonate buffer 6.0 – 7.0
• Sodium acetate buffer 6.0

FAQ and Support

Popular FAQ

Find all FAQ here