ImpaRATOR Lyophilized

1,215.00

Lyophilized enzyme for digestion of mucin-type O-glycoproteins and peptides, including sialylated O-glycan species

1,215.00
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Description

ImpaRATOR is an O-glycan-dependent protease that digests proteins carrying mucin-type O-glycans, including sialylated species, N-terminally of glycosylated Ser and Thr residues.

Detailed Information

Application

Product Group

Source/Origin

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Product Composition

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Digestion Site

Specificity

Substrates

Reaction Conditions

Digestion Buffers

Buffer Compatibility

Inhibitors

Unit Definition

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FAQs

ImpaRATOR is a metalloprotease and thereby sensitive to chelating agents such as EDTA. Concentrations > 1 mM EDTA results in complete inhibition of the enzyme. In addition, the ImpaRATOR activity is inhibited by reducing agents and detergents.

Insufficient digestion during non-denaturing conditions can be caused by O-glycosylation sites within the glycoprotein inaccessible to the enzyme. In such cases, we recommend trying the following workflow: reduction, denaturation, carboxymethylation, buffer-exchange, and then digestion with ImpaRATOR. If detergent is added, make sure to include a detergent removal step prior ImpaRATOR digestion.

ImpaRATOR has a broad activity towards different O-glycan structures; however, the enzyme has limited activity towards sites with two adjacent O-glycosylated Ser/Thr residues. For complete information about O-glycan sites in glycoprotein substrates containing several sites with two adjacent O-glycosylated Ser/Thr residues, OpeRATOR may be a better option.

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