IgGZERO™
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IgGZERO™ Immobilized
Immobilized enzyme for hydrolysis of complex-type Fc N-glycans in spin columns
IgGZERO (EndoS) is an IgG-specific endoglycosidase hydrolyzing complex N-glycans at the Fc N-glycosylation sites.
The IgGZERO Immobilized spin columns contain the IgGZERO enzyme covalently coupled to agarose beads, for deglycosylation of Fc N-glycans without contaminating the final preparation with enzyme.
The spin columns are available for deglycosylation of 0.5 mg (Microspin), 10 mg (Midispin) or 100 mg (Maxispin) IgG. The smallest columns are available in three different pack sizes, with 2, 5 or 10 columns per pack.
Why IgGZERO™ Immobilized?
- Easy-to-use spin columns
- Increased enzyme-to-substrate ratio for improved digestion efficiency
- No enzyme in the final sample
Available Products
IgGZERO Immobilized Microspin 2 x 0.5 mg
Immobilized enzyme for deglycosylation of 2 x 0.5 mg IgG in spin columns
IgGZERO Immobilized Microspin 5 x 0.5 mg
Immobilized enzyme for deglycosylation of 5 x 0.5 mg IgG in spin columns
IgGZERO™ Immobilized Microspin 10 x 0.5 mg
Immobilized enzyme for deglycosylation of 10 x 0.5 mg IgG in spin columns
IgGZERO Immobilized Midispin 1-10 mg
Immobilized enzyme for deglycosylation of 1-10 mg IgG in spin columns
IgGZERO Immobilized Maxispin 10-100 mg
Immobilized enzyme for deglycosylation of 10-100 mg IgG in spin columns
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One spin column contains sufficient IgGZERO-coupled agaose beads to deglycosylate 0.5 mg (Microspin), 10 (Midispin) or 100 (Maxispin) mg IgG.
Content and Storage
IgGZERO Immobilized is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Applications
A simple and rapid estimation of IgG core fucosylation using LC-MS analysis.
FAQ and Support
I did not get complete deglycosylation using IgGZERO Immobilized, how can I improve digestion?
First you could increase incubation time and increase temperature to 37°C. One explanation for incomplete deglycosylation with IgGZERO Immobilized could be that the antibody has higher amounts of high mannose, hybrid glycans or bisected glycans. For these glycans IgGZERO has low or no activity. High mannose glycans are particularly resistant to digestion recommend you try our other endoglycosidase GlycINATOR (EndoS2). This enzyme has much higher activity on these types of glycans sometimes seen in mAbs.
I read that cleavage buffer for IgGZERO is 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 Can I use PBS 1X in place of cleavage buffer?
Yes, many buffers are compatible with IgGZERO. Please keep pH close to neutral.
What is the difference between GlycINATOR Immobilized and IgGZERO Immobilized?
In both products the enzyme is immobilized on agarose beads for IgG-specific hydrolysis of the Fc glycans to the innermost GlcNAc. IgGZERO Immobilized contains IgGZERO (EndoS) and GlycINATOR Immobilized contains GlycINATOR (EndoS2). The GlycINATOR enzyme has much higher enzymatic activity for high mannose and some bisected and hybrid glycans that can occur on mAbs.
Can I use PBS 1X instead of the IgGZERO reaction buffer?
Yes, the IgGZERO enzyme is compatible with most commonly used buffers with pH ranging from 6.0 to 8.0, but the reaction conditions might need to be optimized to ensure optimal deglycosylation.
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