Genovis » IgASAP Sub1+2 Lyophilized

Description

IgASAP Sub1 is an IgA1-specific protease that digests human IgA1 and IgA2m1 at one specific site above the hinge, generating intact and homogeneous Fab and Fc fragments.

Detailed Information

Product Group	IgASAP
Application	Antibody Characterization, Antibody digestion, Antibody Fragmentation
Source/Origin	Cloned from Erysipelatoclostridium ramosum, Produced in E. coli (His-tagged), 131 kDa
Product Composition	Lyophilized in TBS, Purified to ≥ 90% homogeneity
Digestion Site	Above Hinge
Specificity	IgA1: VTVPCP/VPSTPP (above the hinge), IgA2m1: VTVPCP/VPPPPP (above the hinge)
Substrates	Human IgA1, Human IgA2m1
Reaction Conditions	Overnight reaction at 37°C according to protocol specifications
Digestion Buffers	PBS (pH 7.4), TBS (pH 7.6)
Buffer Compatibility	Active at pH 6.0 to 8.0, does not require reducing conditions or co-factors for activity and tolerates salt concentrations up to 0.5 M NaCl
Inhibitors	Activity is affected by ZnCl2 and EDTA (other inhibitors not yet evaluated)
Unit Definition	1 unit digests ≥ 90% of 1 μg human IgA of IgA1 and IgA2m1 type

FAQs

Is there an enzyme for digestion of IgA2-only?

There is no known enzyme that targets IgA2-only since the amino acids in the hinge regions of IgA2 is present also in IgA1. However, IgASAP Sub1 will digest IgA1-only.

What enzyme should be selected for the digestion of IgA1?

IgASAP Sub1 specifically targets IgA1 and exhibits a faster reaction time (1 hour) compared to IgASAP Sub1+2 (16-18 hours). The cleavage site of IgASAP Sub1+2 is located six amino acids above that of IgASAP Sub1, resulting in the absence of potentially O-glycosylated residues on the Fab fragments. This characteristic can be advantageous in certain cases.