FabULOUS Fab kit

795.00

FabULOUS Fab kit generates and purifies Fab and Fc fragments from mouse IgG

795.00
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Description

FabULOUS Fab kit contains FabULOUS (SpeB) enzyme and CaptureSelectTM spin columns for affinity capture of mouse LC-lambda. FabULOUS digests mouse IgG1 above the hinge and other subclasses in the hinge region generating Fc and Fab fragments. CaptureSelectTM spin columns capture mouse LC-lambda for purification of Fab and Fc fragments. FabULOUS Fab kit is available in spin column format for digestion and purification of 2 mg of human IgG1.

Detailed Information

Application

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Product Group

Source/Origin

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Product Composition

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Digestion Site

Specificity

Substrates

Reaction Conditions

Digestion Buffers

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Buffer Compatibility

Additives/Co-factors

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Inhibitors

Unit Definition

FAQs

Yes, FabULOUS requires reduced condition for activity and reaction is performed in the presence of 1-5 mM DTT or TCEP at neutral pH.

FabULOUS enzyme reaction is inhibited with iodoacetamide, iodo acetic acid or lowering pH below 3.

Yes, but a longer incubation time is needed.

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