SEQURNA™ Brings Synthetic RNase Inhibitors to Single-Cell Transcriptomics!

October 21, 2024 | Genomics, News, Products |

Scientists at the Karolinska Institute have demonstrated the construction of single-cell RNA sequencing (scRNA-seq) libraries using SEQURNA RNase Inhibitor Thermostable, a novel, fully synthetic, and thermostable RNase inhibitor. SEQURNA overcomes the limitations of traditional recombinant RNase inhibitors (RRIs) by providing superior thermal stability, enhanced reproducibility, and logistical convenience. Its use enabled the creation of high-quality single-cell libraries with increased experimental flexibility. The introduction of SEQURNA will significantly advance single-cell transcriptomics, making scRNA-seq more reliable and scalable!

Single-cell RNA sequencing (scRNA-seq) is a powerful technique in biomedicine that allows for the analysis of gene expression at the single-cell level. Maintaining RNA integrity during cell collection, storage, and cDNA library generation is crucial for accurate results. Traditionally, protein-based RRIs are used to prevent RNA degradation, but they have limitations such as high cost, thermal sensitivity, and batch variability. SEQURNA is active across a wide temperature range, providing sustainability through ambient storage and shipping, minimizes batch effects, and avoids activity loss from freeze-thaw cycles and long incubation times. This makes it ideal for applications such as single-cell and in situ RNA sequencing, where preserving high-quality RNA is essential.
 

 
Figure 1. Schematic overview of the SEQURNA RNase Inhibitor workflow.
 
The scientists here set out to investigate whether traditional RRIs could be replaced with SEQURNA to create high-quality scRNA-seq libraries. SEQURNA was shown to be fully compatible with all enzymatic reactions required to create sequencing-ready libraries. Furthermore, the thermostability of SEQURNA enabled activity throughout freeze-thawing and PCR cycles, enhancing the reliability, consistency and scalability of scRNA-seq experiments. The thermostability reduces the need for dry ice transport, simplifying the logistics of handling and storage while the chemistry minimizes carryover of other proteins, such as nucleases, from protein production. Usage of SEQURNA produces high-quality single-cell libraries, maintaining RNA integrity better than RRIs and offering greater experimental flexibility.
 
The introduction of SEQURNA represents a significant advancement in the field of single-cell transcriptomics. By addressing the limitations of RRIs, SEQURNA enhances the reliability and efficiency of scRNA-seq, paving the way for more robust and scalable single-cell RNA analysis.
 
Replacing traditional RRIs with SEQURNA offers substantial benefits, including superior thermal stability, enhanced reproducibility, and logistical convenience. This innovation is expected to have a profound impact on single-cell RNA sequencing, making it more accessible and reliable for researchers!
 

Why SEQURNA™?

  • 🔥 Thermostable – active across a wide temperature range, ensuring reliable RNA protection in heat-based workflows, simplifying logistics and enabling flexible protocol development
  • 🧬 Synthetic – reduces batch variability for consistent results, with no risk of introducing cell-based contaminants from production
  • 🎨 Versatile – preserves RNA integrity across various workflows, including single-cell and in situ sequencing
  • 🌱 Sustainable – energy-efficient synthesis and storage, with ambient shipping and room temperature stability – no dry ice shipments!

Reference

Noble et al., 2024. Introducing synthetic thermostable RNase inhibitors to single-cell RNA-seq. Nature Communications

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SEQURNA™ RNase Inhibitor Thermostable – Securing RNA for Sequencing