[GenovisWebinar!] 🤩 Unlocking Antibody Insights with Regeneron: SmartEnzymes™ in Action!
We are thrilled to welcome Xin Wang from Regeneron Pharmaceuticals to showcase how the reliability and robust performance of Genovis’ SmartEnzymes have enabled their subunit HILIC-MS method to be successfully applied across multiple Regeneron programs and commercial mAbs.
By combining SmartEnzymes, Regeneron developed a novel subunit HILIC-MS method for the site-specific localization and quantitation of intra-domain free thiols in mAbs.
- FabRICATOR® digests IgG at a specific site below the hinge, generating homogeneous F(ab’)2 and Fc fragments for middle-level characterization of antibodies.
- GalactEXO™ hydrolyzes b1-3,4-linked terminal galactose on N- and O-glycosylated proteins or oligosaccharides.
This method’s broad applicability validates its exceptional utility as a label-free approach for quantitating free thiols in mAb analysis!
Title:“Development of a Novel Subunit HILIC-MS Method for Site-specific Localization and Quantitation of Intra-domain Free Thiols”
Date: December 11th, 2024
Time: 4 pm CET / 10 am ET
Join us to explore how Regeneron is advancing antibody research with Genovis’ SmartEnzymes!
REGISTER HERE!
Key Learning Objectives
- Learn how FabRICATOR and GalactEXO enable precise identification and localization of intra-chain free thiols in mAbs
- Learn how middle-up HILIC-MS can be used as a label-free method for free thiol quantitation
- Explore the successful application of this method across various Regeneron programs and seven commercial mAbs
Who Should Attend?
- Scientists involved in the MS characterization of biopharmaceuticals
- Scientists working in Quality Control (QC) labs
- Scientists performing middle-level analysis of mAbs
- Contract Research Organizations (CROs)
- Contract Manufacturing Organizations (CMOs)
- Academic scientists working with protein characterization by MS
About FabRICATOR®
FabRICATOR (IdeS) is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.
The enzyme is widely used in characterization, quality control, stability testing, production monitoring and clone selection of antibody-based therapeutics such as mAbs, ADCs, biosimilars and Fc-fusion proteins.
Learn More About FabRICATOR
About GalactEXO™
GalactEXO is a β-Galactosidase mix that hydrolyses β1-3,4-linked terminal galactose on N- and O-glycosylated proteins or oligosaccharides.
The enzyme is applicable for trimming of galactose on released glycan structures for exoglycosidase arrays or for the generation of antibodies with homogenous G0/G0F glycan profiles.
Learn More About GalactEXO