PNGase F
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PNGase F Automation
Lyophilized enzyme in 96-well plates for automated hydrolysis of N-glycans on glycoproteins
PNGase F (Peptide N-glycosidase F) is a glycoamidase hydrolyzing the amide bond between the polypeptide asparagine and the innermost GlcNAc of all mammalian asparagine-linked complex, hybrid, or high-mannose oligosaccharides.
PNGase F Automation contains the PNGase F enzyme lyophilized in an automation-friendly 96-well plate format. It enables high-throughput N-glycan removal to facilitate simplified downstream analysis of a range of glycoprotein substrates. Depending on your workflow requirements and sample availability, PNGase F Automation is available lyophilized in 96-well plates for automated hydrolysis of N-glycans on 96 x 5 μg or 96 x 50 μg glycoprotein.
Why PNGase F Automation?
- Enables automated high-throughput sample processing
- Digestion of up to 96 samples in parallel
- Two options available for digestion of different sample amounts
- Ready to use – just add your sample!
Available Products
PNGase F Automation 96 x 5 units
Lyophilized enzyme in 96-well plates for automated hydrolysis of N-glycans on 96 x 5 𝜇g glycoprotein
PNGase F Automation 96 x 50 units
Lyophilized enzyme in 96-well plates for automated hydrolysis of N-glycans on 96 x 50 𝜇g glycoprotein
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit PNGase F removes N-glycans from ≥ 95% of 1 µg etanercept when incubated in TBS buffer (50 mM Tris-HCl, 150 mM NaCl) pH 8.6 at 37°C for 1 h.
Content and Storage
PNGase F Automation is supplied lyophilized in 50 mM HEPES buffer pH 7.5, with no preservatives added. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
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FAQ and Support
Can I use FabRICATOR and PNGaseF in the same reaction?
Yes, PNGaseF and FabRICATOR can be used in a one-pot reaction as long as the reaction conditions follow the specifications for the FabRICATOR enzyme. It is important to have native reaction conditions for FabRICATOR to remain active.
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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