FabULOUS™ Fab Kit

Lyophilized enzyme and affinity resin for hinge-region digestion of murine IgG and purification of fragments

Available Products

  • Purification

FabULOUS (SpeB) is a cysteine protease that digests in the hinge region of IgG from several different species and subclasses, generating Fab and Fc fragments.

FabULOUS Fab Kit contains one vial of FabULOUS enzyme with 2000 units for digestion of 2 mg murine IgG, and 4 CaptureSelect™ LC-Kappa (mur)* microspin columns for affinity purification of fragments.

The affinity matrix recognizes the LC-Kappa domain of murine IgG antibodies, which enables purification of Fab fragments. Each microspin column contains sufficient material to purify 0.5 mg IgG.

Why FabULOUS™ Fab Kit?

  • Purification of Fab fragments from murine IgG
  • Fast – pure fragments in under two hours
  • Reliable workflow for preparative applications

Available Products

  • FabULOUS™ Fab Kit 2 mg

    Lyophilized enzyme and affinity resin for above hinge digestion of 2 mg murine IgG and purification of fragments

    A1-PFK-020

    €1,079.00

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    For information on how to order, visit Place an Order or contact us directly at order@genovis.com.

    Product Information

    Unit Defintion

    One unit digests ≥ 95% of 1 µg of human IgG1 in PBS pH 7.4 with 5 mM DTT or TCEP at 37˚C for 1 hour. This is also valid for 1 µg of mouse IgG1 in PBS with 30-50 mM L-cysteine as reducing agent.

    Content and Storage

    FabULOUS Fab Kit is shipped cold.The FabULOUS Lyophilized vials should be stored at -20°C upon arrival.

    The microspin columns containing CaptureSelect™* LC-Kappa (mur) resin should be stored at +4-8°C. Do not freeze the spin columns!

    After reconstitution, the FabULOUS enzyme is stable for 1 month at +4-8°C.

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    Performance Activity

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    FAQ and Support

    Yes, but a longer incubation time is needed.

    Yes, FabULOUS should be able to digest a Fc fusion protein as long as you have not fused the protein too close to the cleavage site. FabULOUS digests human IgG1 just above the hinge (…KTHT / CPPCP…).

    FabULOUS enzyme reaction is inhibited with iodoacetamide, iodo acetic acid or lowering pH below 3.

    Yes. GingisKHAN is provided lyophilized. After reconstitution, the enzyme can be used for up to 4 different antibodies. The digestion is performed in solution and you can prepare Fab fragments from 4 x 0.5 mg human IgG1. The purification step is done with Capture Select columns and 4 columns are provided in the kit.

    Depending on what reducing agent and what concentration you use you can obtain intact Fab and not reduce the interchain disulfide bond. The light chain (LC) and heavy chain (HC) will not dissociate under native conditions due to hydrogen/hydrophobic forces. Before you analyze your samples with SDS-PAGE you must remove the cysteine otherwise the Fab will be reduced to Fd and LC during sample preparation for the SDS-PAGE. This can be done by a simple buffer exchange.

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    Legal & Disclaimers

    All rights reserved. Genovis products may be covered by one or more patents, trademarks and copyrights owned by Genovis AB or licensed from third parties. For more information about commercial rights, please contact the Genovis team at licensing@genovis.com.

    Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.

    * Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.

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