MagIC
Immobilized enzyme on magnetic beads for automated digestion of IgG below the hinge. FabRICATOR (IdeS) is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.
The FabRICATOR MagIC magnetic beads contains FabRICATOR Immobilized enzyme. The beads enable fast automated digestion of multiple antibodies in parallel in a middle-level characterization workflow. They are designed for automation platforms, but perform equally well on a shaking table if automation is not available.
After digestion, the samples can be directly transferred to the autosampler of the LC-MS for fast chromatography or to another analytical method for analysis. Automated parallel antibody digestion and subunit generation allow for the processing of the numerous samples associated with clone selection, process development and stability studies for a potential biopharmaceutical. Automated antibody subunit analysis reduces the operator time and the risk for sample handling errors, and also increases the throughput.
- No enzyme in the final sample
- Fast, automated sample preparation
- Digestion of up to 96 samples in parallel
- Applicable to all magnetic automation platforms
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Automated Parallel Analysis of Antibodies
Download product folderStorage
FabRICATOR MagIC is supplied as a 25% bulk resin in vials. The product is shipped cold and should be stored at +4-8°C.
Product Overview
Product size: 2 ml and 4 x 2 ml
Sample size: Digestion of up to 24 x 200 µg IgG (2ml) and 96 x 200 µg IgG (4 x 2 ml)
Short Specification: 20µL settled magnetic agarose resin digest >=95% of 20 and 200µg human IgG1 in 100µL 10 mM Sodium Phosphate, 150 mM NaCl pH 7.4 in 20 minutes at 37°C as monitored by SDS-PAGE
Starting material: Human IgG1-4, Fc-fusion proteins
Operating temperature: 37°C
Storage conditions: +4°-8°C
Bead material: Magnetic agarose beads
Compatible Additives: 5mM TECP for generation of reduced subunits, Fd’, LC and scFc.
0.05% Polysorbate 20 in the digestion buffer to minimize shear stress in automated systems.
Performance
The performance of FabRICATOR MagIC at 37°C is typically less than 20 minute digestion with efficiency of >95% for 20-200 µg human IgG1 (1 mg/ml) sample in PBS, pH 7.4.
Instructions
Product Specifications
Safety Data Sheet
Certificate of Analysis
FAQ and Support
Popular FAQ
The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme.
FabRICATOR MagIC is suitable for using in automated systems that can handle magnetic beads. On the applications page you can read about how it’s used in a KingFisher™ workflow.
FabRICATOR is a cysteine protease. It should not result in any other modifications than the digestion of the peptide bond. Digestion will be between the two Glycines (…CPAPELLG / GPSVF…) and the C-terminus will regain the carboxyl group – that is addition of one OH-group and the N-terminal amino group will be regained (addition of one H).
In summary, R1-CO-NH-R2 + H2O –> R1-COOH + NH2-R2.
The protocol for FabRICATOR MagIC is based on using standard PBS pH 7.4 as digestion buffer. FabRICATOR in solution is compatible with most commonly used buffers at pH=6-8 and the same buffers should work for FabRICATOR MagIC, but the incubation time might need to be optimized.
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