GlySERIAS is a unique enzyme that digests flexible glycine-rich fusion protein linkers such as Gly4Ser and GlyxSery (GS), and polyglycine (G) linkers.
The enzyme enables separation of the individual domains of multi-functional fusion proteins to facilitate characterization and increase the understanding of these molecules. Middle-level analysis of fusion proteins serves to both reduce the overall sample complexity and allows for domain-specific identification and monitoring of post-translational modifications.
Immobilized enzyme for digestion of fusion proteins in spin columns
About GlySERIAS™
GlySERIAS is a unique enzyme for digestion of fusion proteins designed with flexible linkers consisting of repetitive sequences of glycine, or glycine and serine, residues. The enzyme is active under native reaction conditions and enables middle-level characterization of complex fusion proteins.
The linker regions are digested at several sites simultaneously, leading to separation of the previously linked components. Optimal activity occurs at pH 7.6 and the length of the incubation can be varied depending on the desired end-products. For improved linker removal we recommend GlySERIAS Immobilized, for linker characterization we recommend GlySERIAS Lyophilized.
GlySERIAS is cloned from phage K and is expressed in E. coli. The enzyme contains a His-tag and has a molecular weight of 18 kDa.
GlySERIAS™ Lyophilized
Lyophilized enzyme for digestion of fusion proteins.
GlySERIAS Lyophilized is available as a lyophilized powder in 2000 unit vials for digestion of 2 mg fusion proteins.
Flexible format for method development
Possible to combine enzymes for improved efficiency
Amenable to automated workflows
Ready to use – just add water!
Enables linker characterization
Available Products
GlySERIAS Lyophilized 2000 unitsLyophilized enzyme for digestion of 2 mg fusion proteinA0-GS1-020€795.00Buy / Request a Quote
One unit GlySERIAS digests ≥ 95% of 1 µg dulaglutide at a minimum of one site when incubated in TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.6) at 37°C for 15 minutes.
Content and Storage
GlySERIAS is supplied lyophilized in 50 mM Tris-HCl, 150 mM NaCl, pH 7.6, with no preservatives added. Upon arrival, the enzyme should be stored at -20°C. After reconstitution, GlySERIAS is stable for at least 1 month at +4-8°C.
Immobilized enzyme for digestion of fusion proteins in spin columns.
The GlySERIAS Immobilized spin columns contain GlySERIAS enzyme covalently coupled to agarose beads, for digestion of fusion proteins without enzyme in the final preparation.
The Microspin columns are available for digestion of 5 or 10 x 0.2 mg fusion protein.
Easy-to-use spin columns
Increased enzyme-to-substrate ratio for improved digestion efficiency
No enzyme in the final sample
Improved linker removal
Available Products
GlySERIAS Immobilized Microspin 5 x 0.2 mgImmobilized enzyme for digestion of 5 x 0.2 mg fusion protein in spin columnsA0-GS6-010€1,193.00Buy / Request a Quote
GlySERIAS Immobilized Microspin 10 x 0.2 mgImmobilized enzyme for digestion of 10 x 0.2 mg fusion protein in spin columnsA0-GS6-020€1,908.00Buy / Request a Quote
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