Powered by BiozHydrolysis of Fc N-glycans
IgGZERO (EndoS) is an IgG-specific endoglycosidase hydrolyzing complex N-glycans at the Fc N-glycosylation sites.
The enzyme deglycosylates IgG after the core GlcNAc and display limited activity on high-mannose and hybrid-type glycans. The enzyme is used to rapidly reduce sample complexity, to inactivate Fc-mediated effector functions in immunoassays and as a tool to improve imaging by reducing Fc interactions.
- Highly efficient IgG-specific endoglycosidase
- Fast - hydrolyzes complex Fc N-glycoforms within 30 minutes
- Enables characterization and quality control of antibody-based biotherapeutics
- Available immobilized in ready-to-use spin columns
Download product folderIgGZERO® Lyophilized
Lyophilized enzyme for deglycosylation of IgGIgGZERO® Low Endotoxin
Lyophilized enzyme with low levels of endotoxin for deglycosylation of IgGIgGZERO® Immobilized
Immobilized enzyme for deglycosylation of IgG in spin columnsAbout IgGZERO®
IgGZERO (EndoS) is an endoglycosidase from Streptococcus pyogenes that specifically hydrolyzes N-linked glycans in the Fc-region of native IgG.
It can be used to rapidly deglycosylate antibodies to reduce sample complexity, or to inactivate Fc-mediated effector functions.
The enzyme deglycosylates all human IgG subclasses and IgG from many different species, including mouse, rat, monkey, sheep, goat, cow, and horse. The enzyme only hydrolyzes glycans from the Fc-domain of IgG, even when other glycosylated proteins are present in the solution. The enzyme is expressed in E. coli, contains a His-tag, and the molecular weight is 110 kDa.
The IgGZERO enzyme has limited activity on high-mannose and hybrid-type glycans (Sjögren et al., 2015). If you are interested in endoglycosidases that hydrolyze all Fc N-glycoforms on IgG, we recommend the GlycINATOR product family.
Sjögren, J. et al., 2015. EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans. Glycobiology, 25(10), pp.1053–1063.
IgGZERO® Citations
IgGZERO® Lyophilized
Lyophilized enzyme for deglycosylation of IgG.
IgGZERO Lyophilized is available as a lyophilized powder in two different sizes; 1000 and 5000 units, for deglycosylation of 1 or 5 mg IgG, respectively.
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Deglycosylation of IgG
Degree of Fucosylation in Clone SelectionUnit Definition
One unit deglycosylates ≥ 95% of 1μg human IgG, when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30 min.
Content and Storage
IgGZERO Lyophilized is reconstituted by addition of water. No preservatives added. After reconstitution, IgGZERO Lyophilized is stable for 1 month at +4-8°C. The product is shipped at ambient temperature and should be stored at -20°C upon arrival.
Instructions
Product Specification
Safety Data Sheet
Certificate of Analysis
IgGZERO® Low Endotoxin
Lyophilized enzyme with low levels of endotoxin for deglycosylation of IgG.
IgGZERO Low Endotoxin is available as a lyophilized powder in 2000 unit vials for deglycosylation of 2 mg IgG. This product formulation has low levels of endotoxins per vial (< 0.2 EU).
- Efficient digestion with documented low levels of endotoxin
- Suitable for use in sensitive in vivo or cell-based assays
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Unit Definition
One unit deglycosylates ≥ 95% of 1μg human IgG when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30 min.
Content and Storage
IgGZERO Low Endotoxin is reconstituted by addition of water. No preservatives added. After reconstitution, IgGZERO Low Endotoxin is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
Instructions
Product Specification
Safety Data Sheet
Certificate of Analysis
IgGZERO® Immobilized (formerly known as deGlycIT)
Immobilized enzyme for deglycosylation of IgG in spin columns.
The IgGZERO Immobilized spin columns contain the IgGZERO enzyme covalently coupled to agarose beads, for deglycosylation of Fc N-glycans without contaminating the final preparation with enzyme.
The spin columns are available for deglycosylation of 0.5 mg (Microspin), 10 mg (Midispin) or 100 mg (Maxispin) IgG. The smallest columns are available in three different pack sizes, with 2, 5 or 10 columns per pack.
- Easy-to-use spin columns
- Increased enzyme-to-substrate ratio for improved digestion efficiency
- No enzyme in the final sample
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Unit Definition
One spin column contains sufficient IgGZERO-coupled agaose beads to deglycosylate 0.5 mg (Microspin), 10 (Midispin) or 100 (Maxispin) mg IgG.
Content and Storage
IgGZERO Immobilized is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!
Instructions
Product Specification
Safety Data Sheet
Certificate of Analysis
FAQ and Support
Popular FAQ
Yes, the IgGZERO enzyme is compatible with most commonly used buffers with pH ranging from 6.0 to 8.0, but the reaction conditions might need to be optimized to ensure optimal deglycosylation.
Yes, many buffers are compatible with IgGZERO. Please keep pH close to neutral.
First you could increase incubation time and increase temperature to 37°C. One explanation for incomplete deglycosylation with IgGZERO Immobilized could be that the antibody has higher amounts of high mannose, hybrid glycans or bisected glycans. For these glycans IgGZERO has low or no activity. High mannose glycans are particularly resistant to digestion recommend you try our other endoglycosidase GlycINATOR (EndoS2). This enzyme has much higher activity on these types of glycans sometimes seen in mAbs.
In both products the enzyme is immobilized on agarose beads for IgG-specific hydrolysis of the Fc glycans to the innermost GlcNAc. IgGZERO Immobilized contains IgGZERO (EndoS) and GlycINATOR Immobilized contains GlycINATOR (EndoS2). The GlycINATOR enzyme has much higher enzymatic activity for high mannose and some bisected and hybrid glycans that can occur on mAbs.
Typically, GlycINATOR is the first choice since it displays enzymatic activity on all glycoforms present on IgG, including complex type, high mannose, bisected and hybrid type glycans. IgGZERO does not hydrolyze high mannose glycans. IgGZERO deglycosylates some species faster as compared to GlycINATOR, for example goat IgG.
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