Powered by BiozAbove Hinge Digestion of Human IgG1
FabALACTICA (IgdE) is a cysteine protease that digests human IgG1 at a specific site above the hinge, generating intact and homogenous Fab and Fc fragments.
The enzyme facilitates the characterization of bi- and multispecific antibodies and analyses of intact and paired Fc glycosylation. It also simplifies the study of monovalent binding, higher order structures, disulfide scrambling and antibodies with mutated hinge regions.
- Specific - one digestion site above the hinge of human IgG1
- Generates homogeneous Fab and Fc fragments with preserved immunoreactivity
- Middle-level characterization of human IgG1
- Available immobilized in ready-to-use spin columns
FabALACTICA® Lyophilized
Lyophilized enzyme for above hinge digestion of human IgG1FabALACTICA® Immobilized
Immobilized enzyme for above hinge digestion of human IgG1 in spin columnsFabALACTICA® Fab Kit
Immobilized enzyme and affinity resin for above hinge digestion of human IgG1 and purification of Fab fragmentsAbout FabALACTICA®
FabALACTICA is a cysteine protease that digests human IgG1 at one specific site above the hinge (KSCDKT / HTCPPC), generating intact Fab and Fc fragments.
The name of the enzyme is derived from the pathogen Streptococcus agalactiae, where it was first discovered (Spoerry et al., 2016). The enzyme is active at pH from 6-8 and does not require reducing conditions or co-factors for activity.
FabALACTICA is recombinantly expressed in E. coli, contains a His-tag, and the molecular weight is 70 019 Da.
Spoerry, C. et al., 2016. Novel IgG-Degrading Enzymes of the IgdE Protease Family Link Substrate Specificity to Host Tropism of Streptococcus Species. PloS one, 11(10), p.e0164809.
FabALACTICA® Citations
FabALACTICA® Lyophilized
Lyophilized enzyme for above hinge digestion of human IgG1.
FabALACTICA Lyophilized is available as a lyophilized powder in 2000 unit vials for digestion of 2 mg human IgG1.
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
FabALACTICA Lyophilized Citations
Unit Definition
One unit digests ≥ 90% of 1 μg human IgG1, when incubated in 150 mM sodium phosphate, pH 7.0 at 37°C overnight (16-18 h).
Content and Storage
FabALACTICA Lyophilized is reconstituted by addition of water. No preservatives added. After reconstitution, the enzyme is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
Instructions
Product Specifications
Safety Data Sheet
Certificate of Analysis
FabALACTICA® Immobilized
Immobilized enzyme for above hinge digestion of human IgG1 in spin columns.
The FabALACTICA Immobilized spin columns contain FabALACTICA enzyme covalently coupled to agarose beads, for digestion of human IgG1 without contaminating the final preparation with enzyme.
The spin columns are available for digestion of up to 0.5 mg (Microspin), 10 mg (Midispin) or 100 mg (Maxispin) human IgG1. The smallest columns are available in two different pack sizes, with 2 or 10 columns per pack.
- Easy-to-use spin columns
- Increased enzyme-to-substrate ratio for improved digestion efficiency
- No enzyme in the final sample
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
FabALACTICA Immobilized Citations
Unit Definition
One spin column contains sufficient FabALACTICA-coupled agarose beads to digest 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) human IgG1.
Content and Storage
FabALACTICA Immobilized is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!
Instructions
Product Specifications
Safety Data Sheet
Certificate of Analysis
FabALACTICA® Fab Kit
Immobilized enzyme and affinity resin for above hinge digestion of human IgG1 and purification of Fab fragments
FabALACTICA Fab Kit consists of spin columns of FabALACTICA Immobilized for antibody digestion, and spin columns of CaptureSelect™* Fc resin for purification of the Fab fragments. One spin column contains sufficient FabALACTICA-coupled agarose beads to process 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) human IgG1.
- Purification of homogeneous Fab fragments from human IgG1
- Reliable workflow for preparative applications
- Enables chain pairing characterization of bispecific antibodies
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
FabALACTICA Fab Kit Citations
Unit Definition
One spin column contains sufficient FabALACTICA-coupled agarose beads to digest 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) human IgG1.
Content and Storage
The FabALACTICA Immobilized spin columns and CaptureSelect™* spin columns are supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the products!
Product Specifications
Safety Data Sheet
Certificate of Analysis
* Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.
FAQ and Support
Popular FAQ
It is not recommended to elute the Fc fragments from the CaptureSelect column since the fraction might also include undigested or semi digested material.
No, FabALACTICA has shown very poor activity on Fc-fusion proteins.
We recommend using FabALACTICA Immobilized for digesting human IgG1 and generating a final preparation free of any residual enzyme. You can also use FabALACTICA Fab kit for digestion of human IgG1 and purification of the generated Fab fragments.
We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.
FabALACTICA does not require reducing conditions as both GingisKHAN and FabULOUS does, and allows for generation of Fab fragments from native human IgG1.
Support