Powered by BiozDigestion of IgM
IgMBRAZOR is a unique IgM-specific protease that digests human IgM at one specific site below the CH2 domain in the heavy chain, generating homogeneous F(ab’)2 and Fc fragments.
The enzyme enables middle-level analysis of the complex and high molecular weight human IgM, which facilitates IgM characterization during, for example, the development of vaccines, therapeutics, and diagnostics.
- Unique - enables middle-level characterization and quality control of IgM
- Fast – generates homogeneous F(ab’)2 and Fc fragments within 30 minutes
- Highly specific – one digestion site in the heavy chain of human IgM
IgMBRAZOR™ Lyophilized
Lyophilized enzyme for digestion of IgMAbout IgMBRAZOR™
IgMBRAZOR is a unique IgM-specific enzyme that digests human IgM at one specific site below the CH2 domain in the heavy chain, generating F(ab’)2 and Fc fragments.
The digestion is complete within 30 minutes, and due to the specificity of the enzyme, there is no risk of overdigestion if the incubation time is prolonged. The enzyme is active at pH 5.5 to 9.0 and does not require reducing conditions or co-factors for activity. The optimal activity is at 37°C, but the digestion can also be performed at room temperature using an increased incubation time.
IgMBRAZOR is expressed in E. coli. The enzyme contains a His-tag and has a molecular weight of 38 kDa.
IgMBRAZOR™ Citations
IgMBRAZOR™ Lyophilized
Lyophilized enzyme for digestion of IgM.
IgMBRAZOR Lyophilized is available as a lyophilized powder in 1000 unit vials for digestion of 1 mg human IgM.
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Unit Definition
One unit IgMBRAZOR digests ≥90% of 1 µg human myeloma IgM when incubated in PBS (10 mM sodium phosphate, 140 mM NaCl, 2.7 mM KCl), pH 7.4 at room temperature for 20 minutes.
Content and Storage
IgMBRAZOR Lyophilized is reconstituted by the addition of water. No preservatives added. After reconstitution, the enzyme is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
Instructions
Product Specification
Safety Data Sheet
Certificate of Analysis
FAQ and Support
Popular FAQ
Yes, IgMBRAZOR and PNGase F can be used in combination if the reaction is performed under non-denaturing conditions. However, for complete deglycosylation of IgM using PNGase F denaturing conditions may be required and it is then recommended to perform the IgMBRAZOR digestion prior the PNGase F deglycosylation.
It is possible to use IgMBRAZOR with low concentrations of IgM. However, this will lead to a decreased reaction rate, so for IgM concentrations below 0.5 mg/mL it is recommended to increase the amount of added enzyme and/or increase the incubation time. There is no risk of overdigestion and the incubation time can be prolonged to overnight if necessary.
Yes, IgMBRAZOR and FabRICATOR can be used in combination.
The enzyme is active at pH 5.5 to 9.0, tolerates up to 300 mM NaCl, and does not require reducing conditions or co-factors for activity. PBS and TBS buffers are both compatible. Optimization may be required if buffers other than the recommended are used.
IgM from some species of monkey may also be digested by IgMBRAZOR.
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