SmartEnzymes GingisREX workflow

Arginine-specific Protein Digestion

GingisREX (RgpB) is an arginine-specific protease that digests proteins C-terminally of arginine residues, including Arg-Pro linkages that are difficult to digest with other enzymes.

The enzyme can be used in peptide mapping, de novo peptide sequencing and analysis of post-translational modifications.
 

  • Specific digestion C-terminally of arginine residues
  • Reliable digestion at the otherwise difficult arginine-proline sequences
  • Generates longer peptides - improved sequence coverage
Digests any peptide or protein containing arginine, including Arg-Pro motifs
60 min reaction
Active in 6 M urea and 0.1% SDS
Digests C-terminally of arginine residues

GingisREX® Lyophilized

Lyophilized enzyme for digestion C-terminally of arginine residues

About GingisREX®

GingisREX workflow

GingisREX is a cysteine protease that specifically digests peptide bonds C-terminally to arginine residues, including arginine linked to proline. Cysteine is required for enzymatic activity.

Longer peptides are generated, as compared to trypsin digestion, which can be resolved and identified by high-resolution MS. This offers more options in sample preparation for bottom-up approaches, to achieve alternative digestion profiles and increased sequence coverage. GingisREX does not have activity at lysines, as commonly observed using Arg-C. The enzyme is active at a broad pH range of 5.0 - 9.0, it requires cysteine and is inhibited by guanidine hydrochloride.

The enzyme is purified from Porphyromonas gingivalis.

GingisREX® Lyophilized

Lyophilized enzyme for digestion C-terminally of arginine residues.
GingisREX Lyophilized is available as a lyophilized powder in vials of 5 μg enzyme.
 

  • Flexible format for method development
  • Possible to combine enzymes for improved efficiency
  • Amenable to automated workflows
  • Ready to use – just add water!

Available Products

GingisREX Lyophilized 5 ugLyophilized enzyme for digestion C-terminally of arginine residuesB0-GRX-005607.00Buy / Request a Quote

For information on how to order, visit Place an Order or contact us directly at order@genovis.com.

Quality Control Assay

The activity and specificity of GingisREX is tested on oxidized insulin β-chain at a protease:substrate ratio of 1:20 and 1:200. The digestion reaction is performed at 37°C for 30 min and 18 h and analysed with RP-HPLC.

Buffers and Reducing Agent

To maintain the enzymatically active cysteine in a reduced form, TCEP is added to preserve the reducing environment (see user instructions below for details). GingisREX is active in a broad pH range, 5.0-9.0, with optimal activity between pH 6.5-8.0. Digestion can be performed in the presence of chaotropic agents or detergents such as urea, SDS, tween and SDC. Buffers tested and shown to be compatible with GingisREX are Tris, Bis-Tris and ammonium bicarbonate.

Content and Storage

GingisREX Lyophilized is reconstituted by addition of water. No preservatives added. GingisREX is shipped cold and should be stored at -20°C upon arrival. After reconstitution, the enzyme is stable for at least 1 month at 4-8°C.

Instructions

Product Specification

Safety Data Sheet

Certificate of Analysis

FAQ and Support

Popular FAQ

No, GingisREX is inhibited by GdHCl even in low concentrations. This chaotrope should be avoided.

The digestion time is affected by many factors like the enzyme to sample ratio, pH of the digestion reaction, and the sample characteristics. The reaction time needs to be optimized for each specific case.

Yes, cysteine is required for both activity and specificity of the GingisREX enzyme.

 

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