Powered by BiozHydrolysis of Core 1 O-glycans
OglyZOR is an endoglycosidase (O-Glycosidase) that specifically hydrolyzes core 1 O-glycans on native glycoproteins.
OglyZOR is used for removal of O-glycans for glycan analysis, confirmation of O-glycan presence and reduction of sample heterogeneity.
- Highly efficient O-glycosidase
- Reduces sample heterogeneity to unmask potentially critical protein modifications
- Complete and robust removal of O-glycan core 1 disaccharides
Download product folderOglyZOR® Lyophilized
Lyophilized enzyme for hydrolysis of core 1 O-glycans on glycoproteinsAbout OglyZOR®
OglyZOR is an endoglycosidase (endo-α-N-Acetylgalactosaminidase) that catalyzes the hydrolysis of core 1, and to a limited extent core 3, O-glycans from native glycoproteins.
Removal of sialic acids is required for OglyZOR enzyme activity, and therefore, SialEXO Lyophilized composed of a mix of sialidases for removal of α2-3, α2-6 and α2-8-linked sialic acids, is supplied together with OglyZOR. Optimal activity is obtained at pH 6.5 to 7.5 and at 37°C.
OglyZOR is derived from Streptococcus oralis and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 227 kDa. SialEXO is derived from Akkermansia muciniphila and expressed in E. coli. The SialEXO enzymes contain His-tags and the molecular weights of the components are 42 kDa and 66 kDa, respectively.
OglyZOR® Citations
OglyZOR® Lyophilized
Lyophilized enzyme for hydrolysis of core 1 O-glycans on glycoproteins.
OglyZOR Lyophilized is available as a lyophilized powder in 2000 unit vials for hydrolysis of core 1 O-glycans on 2 mg glycoprotein. It requires removal of sialic aids for activity, and is therefore provided together with 2000 units of SialEXO Lyophilized.
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Efficient O-glycan Hydrolysis
Unit Definition
One unit digests ≥ 90% of O-glycans of 1 µg glycoprotein (TNFR) when incubated together with one unit of SialEXO in 20 mM Tris pH 6.8 at 37°C for 2 h.
Content and Storage
OglyZOR Lyophilized includes:
- 1 vial of OglyZOR Lyophilized (G1-OG1-020) supplied in TBS pH 7.6, with no preservatives added.
- 1 vial of SialEXO Lyophilized (G1-SM1-020) supplied in TBS pH 7.6, with no preservatives added.
The product is shipped at ambient temperature, and should be stored at -20°C upon arrival. After reconstitution, the enzymes are stable for 1 month at +4-8 °C.
Instructions
Product Specification
Safety Data Sheet
Certificate of Analysis
FAQ and Support
Popular FAQ
No, OglyZOR recognizes the core 1 disaccharide HexNAcHex (or GalNAcGal) and to some extend also the core 3 disaccharide HexNAc(2) (or GalNAcGlcNAc).
Yes, this workflow enables MS/MS of O-glycosylated peptides with removed O-glycans.
OglyZOR only works on desialylated O-glycans therefore is a sialidase (SialEXO) included with the product. SialEXO is not O-glycan specific and will therefore desialylate any N-glycans that are present.
Yes, we have performed successful experiments with OglyZOR, SialEXO and PNGaseF in the same reaction under native conditions O/N at pH ~7.0. If denaturing reaction conditions are required for PNGaseF, the reaction with OglyZOR and SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.
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