Thermostable RNase Inhibitor
Securing RNA for Sequencing
SEQURNA® Thermostable RNase Inhibitor is a fully synthetic and heat-tolerant inhibitor that protects RNA integrity. It is active across a wide temperature range, provides sustainability through ambient storage and shipping, minimizes batch effects, and avoids activity loss from freeze-thaw cycles and long incubation times. This makes it ideal for applications such as single-cell and in situ RNA sequencing, where preserving high-quality RNA is essential.
By preserving RNA during crucial steps like cell lysis, RNA storage, and reverse transcription, the inhibitor ensures that sensitive samples remain intact, which is critical in workflows that require precise RNA quantification and sequencing, such as single-cell RNA-sequencing and spatial transcriptomics. SEQURNA Thermostable RNase Inhibitor enhances reproducibility and data accuracy, streamlines experimental workflows, and expands the potential for developing novel protocols that include temperature variations.
- Thermostable – active across a wide temperature range, ensuring reliable RNA protection in heat-based workflows, simplifying logistics and enabling flexible protocol development
- Synthetic - reduces batch variability for consistent results, with no risk of introducing cell-based contaminants from production
- Versatile - preserves RNA integrity across various workflows, including single-cell and in situ sequencing
- Sustainable - energy-efficient synthesis and storage, with ambient shipping and room temperature stability - no dry ice shipments!
SEQURNA® Thermostable RNase Inhibitor
Synthetic thermostable RNase inhibitor ideal for single-cell and in situ RNA sequencingAbout SEQURNA® Thermostable RNase Inhibitor
SEQURNA Thermostable RNase Inhibitor consists of a proprietary mix of synthetic molecules that protects RNA by interfering with the active site of RNases. It is the ideal choice in library preparation for single-cell RNA sequencing, in situ RNA sequencing, multiomics applications, or other methods that rely on consistent RNase inhibition.
The SEQURNA RNase Inhibitor is thermostable, enduring 55°C for 24 hours or 70°C for 2 hours, and can tolerate even harsher experimental conditions. This ensures robust RNA protection throughout and after heat cycles, outperforming commonly used protein-based recombinant RNase inhibitors. The stability of the inhibitor enables the development of new workflows and enhances RNA integrity over longer experimental timelines, as well as during sample handling and storage.
SEQURNA Thermostable RNase Inhibitor tolerates freeze/thaw cycles and vigorous vortexing, requires no DTT, and remains active across a broad pH range of 4.0-10.0.
SEQURNA® Thermostable RNase Inhibitor
Synthetic thermostable RNase inhibitor ideal for single-cell and in situ RNA sequencing.
SEQURNA Thermostable RNase Inhibitor is supplied as a solution in vials of 150 or 3 x 150 μl, with a concentration of 50 mass units/μl.
- Thermostable RNase inhibitor – active across a wide temperature range
- Robust synthetic RNase inhibitor
- Requires no DTT or other toxic reducing agents
- Can easily be stored at room temperature
- Minimizes batch effects in RNase inhibition
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Published Article:
Introducing synthetic thermostable RNase inhibitors to single-cell RNA-seq
Noble et al., 2024. Nature Communications
Detailed SmartSeq Protocols
In these modified Smart-seq protocols, SEQURNA Thermostable RNase Inhibitor replaces the recombinant RNase inhibitor used in the original protocols. The key difference is that SEQURNA is added only to the cell lysis buffer (the cell collection buffer) and not reintroduced during Reverse Transcription. Unlike the protein-based inhibitor, which loses activity at elevated temperature, SEQURNA remains effective throughout the 72°C cell lysis and RNA denaturation step, as well as the RT reaction.
Unit Definition
One unit SEQURNA Thermostable RNase Inhibitor is defined as a mass unit. 5 mass units/µl inhibits 0.1 pg of RNase A or RNase B in a 10 µl reaction (200 ng ssRNA) by ≥ 50% when incubated at 37°C for 1 hour.
Content and Storage
SEQURNA Thermostable RNase Inhibitor is supplied as an aqueous solution with a concentration of 50 mass units/ul. The product is shipped at ambient temperature, and should be stored at room temperature (15-30°C) upon arrival. It also tolerates deep freezing.
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