Question 1

Can I use SialEXO in combination with OglyZOR or OpeRATOR?

kk · Accepted Answer

Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.

Question 2

Does SialEXO contain a purification tag?

kk · Accepted Answer

Yes, SialEXO contains a 6x His-tag.

Question 3

Can you combine GalactEXO with SialEXO or SialEXO 23 to remove both galactoses and sialic acids in a single reaction?

kk · Accepted Answer

Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.

Question 4

Can I use SialEXO for desialylation of both N- and O-glycans?

kk · Accepted Answer

Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.

Question 5

Does SialEXO require denaturation of the proteins for activity on glycoproteins?

kk · Accepted Answer

No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.

Question 6

Does SialEXO require denaturation of the proteins for activity on glycoproteins?

kk · Accepted Answer

No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.

Question 7

Will OglyZOR work on extended core 1 structures or single HexNAc?

kk · Accepted Answer

No, OglyZOR recognizes the core 1 disaccharide HexNAcHex (or GalNAcGal) and to some extend also the core 3 disaccharide HexNAc(2) (or GalNAcGlcNAc).

Question 8

Can OglyZOR be used to deglycosylate peptides generated with OpeRATOR?

kk · Accepted Answer

Yes, this workflow enables MS/MS of O-glycosylated peptides with removed O-glycans.

Question 9

Will OglyZOR leave the N-glycans intact?

kk · Accepted Answer

OglyZOR only works on desialylated O-glycans therefore is a sialidase (SialEXO) included with the product. SialEXO is not O-glycan specific and will therefore desialylate any N-glycans that are present.

Question 10

Can I use OglyZOR and PNGaseF in the same reaction?

kk · Accepted Answer

Yes, we have performed successful experiments with OglyZOR, SialEXO and PNGaseF in the same reaction under native conditions O/N at pH ~7.0.  If denaturing reaction conditions are required for PNGaseF, the reaction with OglyZOR and SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.

Question 11

Can I use OglyZOR and PNGaseF in the same reaction?

kk · Accepted Answer

Yes, we have performed successful experiments with OglyZOR, SialEXO and PNGaseF in the same reaction under native conditions O/N at pH ~7.0.  If denaturing reaction conditions are required for PNGaseF, the reaction with OglyZOR and SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.

Question 12

There are not yet any FAQ's for this product

kk · Accepted Answer

If you have a technical question, please contact us.

Question 13

There are not yet any FAQ's for this product

kk · Accepted Answer

If you have a technical question, please contact us.

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