GingisREX

475.00

GingisREX specifically digests proteins and peptides at arginine residues

475.00
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Description

GingisREX (RgpB) is a cysteine protease that digests any protein and peptide specifically C-terminally of arginine residues. The enzyme is active on difficult to digest Arg-Pro linkages and has no acitivty against lysine residues. GingisREX is available as lyophilized powder for digestion at a ratio of 1:20 – 1:200 enzyme to protein.

Detailed Information

Application

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Product Group

Source/Origin

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Product Composition

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Digestion Site

Specificity

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Reaction Conditions

Digestion Buffers

Buffer Compatibility

Additives/Co-factors

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Inhibitors

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Unit Definition

FAQs

No, GingisREX shows significantly reduced activity in the presence of Gd-HCl even in low concentrations. This chaotrope should be avoided.

Yes, cysteine is required for both activity and specificity of the GingisREX enzyme.

The digestion time is affected by many factors like the enzyme to sample ratio, pH of the digestion reaction, and the sample characteristics. The reaction might need to be optimized for each specific case. In general, we recommend starting with an overnight digestion.

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