€987.00
Affinity purified, biotin-conjugated goat polyclonal antibody targeting the FabRICATOR Z enzyme.
Anti-FabRICATOR Z Affinity Purified Biotin Conjugated is an affinity purified, biotin-conjugated goat polyclonal antibody using FabRICATOR Z as the antigen. It is supplied as a frozen solution of 100 µl with a concentration of 0.5 mg/ml, with no preservatives added.
| Application | |
|---|---|
| Product Group | |
| Source/Origin | |
| Product Composition | A frozen solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4) of 0.1 ml with a concentration of 1 mg/ml (Affinity Purified) or 0.5 mg/ml (Affinity Purified Biotin Conjugated), No preservatives added |
| Digestion Site | Binding: Goat polyclonal IgG binding to the FabRICATOR Z enzyme |
| Specificity | Affinity for FabRICATOR Z (IdeZ) and containing biotin (Affinity Purified Biotin Conjugated) |
| Substrates |
No, FabRICATOR Z only digests mouse IgG2a (….CPAPNLLG / GPSVF.. ) and IgG3 (…CPAPNILG / GPSVF…) at one specific site below the hinge. If Fab fragments are an option, we recommend our FabULOUS enzyme that digest mouse IgG1 above the hinge. FabULOUS requires light reducing conditions for optimal activity. If strong reducing conditions are used, this may likely cause reduction of the interchain disulfides.
Unfortunately, FabRICATOR Z does not digest rat IgG2a. If generating Fab fragments is an option, we suggest using FabULOUS enzyme that digests rat IgG2a. FabULOUS does not have a single specific cleavage site, but a primary digestion site, so there can be more than one digestion site. The digestion site(s) for rat IgG2a using FabULOUS is not determined, but the generated fragments are Fab and Fc.
We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.
It is possible to elute the Fc fragments from the CaptureSelect column using a low pH buffer, for example a 0.1M Glycine buffer pH=3.0. The pH must immediately after elution be adjusted to neutral pH by adding 0.1x elution volume of 1M Tris pH=8.0.