The Genovis team has published a review on FabRICATOR (IdeS) characterization of therapeutic antibodies
The Genovis team has together with Alain Beck, Senior Director Antibody Physico-Chemistry at Pierre Fabre, published a review article summarizing all the current publications using FabRICATOR (IdeS) for characterization of therapeutic antibodies and related products.
“The specificity and robustness of the IdeS digestion is critical for generating a homogenous pool of fragments. Combined with the limited sample preparation and ease of data interpretation, the IdeS enzyme has transformed antibody subunit analysis” Sjögren et al. 2016.
The review is published in The Analyst and demonstrates the wide use of FabRICATOR to study critical antibody qualities such as:
- Fc-glycosylation
- Oxidation
- Drug antibody ratio on ADCs
- Deamidation
The FabRICATOR enzyme specifically digests antibodies into F(ab’)2 and Fc/2 fragments that can be reduced to antibody subunits (Fd, LC and Fc/2) and studied with high mass accuracy.
Learn more on the FabRICATOR enzyme
