Articles tagged ”FabRICATOR Z”

IgG Proteases for Gene Therapy Applications

June 16, 2021 | Applications |

The development and successful commercialization of gene therapy technologies as new treatments have spurred great interest and investments in this field of research. Many challenges remain to be solved to establish gene therapy as a mainstream line of treatment, but there are currently many promising and life-changing gene therapy programs in preclinical and clinical development. Preexisting antibodies against the capsid of adeno-associated virus (AAV) vectors is a major challenge for many gene therapies that could block uptake and limit the transduction of the new genetic material. The anti-AAV antibodies are prevalent both in humans and preclinical disease models. In patient populations, up to 50% of the patients could be excluded from treatment due to high titers of anti-AAV antibodies. Several strategies are explored to reduce the antibody response, once such is enzymatic pretreatment using IgG-specific proteases.
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Now Available – FragIT™Z & FragIT™Z Kit

May 28, 2015 | Products |

We like to introduce FragIT Z – the immobilized version of FabRICATOR Z. Digest your mouse IgG2a more rapidly without having to remove the enzyme from the final preparation.

For separation of the generated antibody domains we also offer the convenient FragIT Z Kit which enables rapid isolation of F(ab’)2 and Fc.

Read more about these products here

Introducing FabRICATOR Z for mouse IgG2a

May 5, 2015 | Products |

We are proud to present a new member of the SmartEnzyme family: FabRICATOR Z (IdeZ).

FabRICATOR Z (IdeZ) is a cystein protease highly similar to FabRICATOR (IdeS). However, FabRCATOR Z is much more efficient in digesting mouse IgG2a and IgG3 than FabRICATOR. The new enzyme is as easy to use as “regular” FabRICATOR – add the enzyme to your mouse IgG and incubate 2 hours to get a homogenous pool of F(ab’)2 and Fc-fragments.

Read more about FabRICATOR Z here