[New Reference] Using HILIC to study antibody subunits
Researchers at the University of Geneva led by Davy Guillarme has used a novel form of HPLC column, based on wide-pore hybrid silica bonded with amide ligand, and developed an HILIC analytical workflow for characterization of biopharmaceuticals. The scientists used FabRICATOR to fragment trastuzumab to subunits and studied the fragments using the new column with HILIC separation. The results showed that the method effectively separated important glycoforms of the Fc/2 fragment. The developed method is compatible with mass spectrometry, does not need high mobile phase temperatures, and allows serial coupling of columns.