Lysine-specific Protein Digestion
LysCERATOR (Lys-C) is a lysine-specific endopeptidase that digests proteins C-terminally of lysine residues, including at lysine-proline linkages.
LysCERATOR is recombinantly produced to high purity and is ideally suited for mass spectrometry-based proteomics applications. Additionally, the enzyme supports peptide mapping, de novo sequencing and characterization of post-translational modifications.
- Best-in-class digestion efficiency at lysine residues minimizes missed cleavages
- Superior digestion at lysine-proline linkages
- Retains high activity under strong denaturing conditions
Download product folderLysCERATOR™ Lyophilized
Lyophilized enzyme for lysine-specific protein digestionAbout LysCERATOR™
LysCERATOR (Lys-C) is a recombinantly produced lysyl endopeptidase that specifically digests peptide bonds C-terminally to lysine residues, including at lysine-proline linkages.
LysCERATOR is a highly efficient enzyme and ensures consistent and specific peptide cleavage whilst minimizing missed cleavages. By generating longer peptides than trypsin, LysCERATOR provides a complementary approach for peptide mapping experiments and can help improve overall sequence coverage. The enzyme is active in a pH range of 7.0 – 9.0 and retains high activity under denaturing conditions up to 8 M urea and 2 M guanidine hydrochloride.
LysCERATOR™ Lyophilized
Lyophilized enzyme for lysine-specific protein digestion.
LysCERATOR Lyophilized is available as a lyophilized powder in vials of 20 μg enzyme.
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Superior Digestion Efficiency on HeLa Cell LysatesQuality Control Assay
0.01 μg LysCERATOR Lyophilized digests ≥ 90% of 1 μg human IgG1 when incubated in 100 mM Tris pH 8.0 including 4 M Urea at 37°C, for 2 hours.
Buffers and Reducing Agent
LysCERATOR is optimally active in Tris and HEPES buffers in a pH range of 7.0 – 9.0. Activity is retained under denaturing conditions up to 8 M urea and 2 M guanidine hydrochloride.
Content and Storage
LysCERATOR Lyophilized is supplied lyophilized in 50 mM HEPES, 100 mM NaCl, 2% trehalose, pH 8.5, with no preservatives added. It is shipped cold, and should be stored at -20°C upon arrival. After reconstitution, LysCERATOR Lyophilized is stable for 4 weeks at -20°C or for 1 week at +4-8°C.
LysCERATOR Lyophilized is for R&D use only.
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