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Ultrafast SmartEnzymes™ Digestion Speed up Antibody Characterization Tell us about your project! We are pleased to showcase our most recent work that completely automates antibody characterization workflows using Genovis SmartEnzymes!…
/smartenzymes/applications/smartstories/IgASAP
IgASAP Sub1 digest human IgA1-only? IgASAP Sub1 may digest IgA1 from other primate species with similar hinge sequences, but this has not been tested. IgASAP Sub1 does not digest IgA2….
/smartenzymes/antibody-digestion/igasap/mAb Quality in Cell Culture
importance of controlling critical quality attributes during the process development, that could avoid extensive product characterization. Zupke, C. et al., 2015. Real-time product attribute control to manufacture antibodies with defined…
/mab-quality-in-cell-culture/FabRICATOR Z
B Can I release Fc fragments from the CaptureSelect column after isolating the F(ab’)2 fragments? It is possible to elute the Fc fragments from the CaptureSelect column using a low…
/smartenzymes/antibody-digestion/fabricator-z/GlycINATOR
a broader substrate selectivity and therefore much higher enzymatic activity on high mannose and some bisected and hybrid glycans that can occur on mAbs. IgGZERO deglycosylates some species faster as…
/smartenzymes/antibody-deglycosylation/glycinator/Generating F(ab´)2 and Fc/2 Antibody Fragments
reducing agent The solution contains Fab’ fragments, Fc fragments, 2-MEA, and potentially FabRICATOR enzyme if FabRICATOR Immobilized was not used. To remove the 2-MEA a desalting column with a suitable…
/smartenzymes/applications/generating-fab2-and-fc-2-antibody-fragments/IgGZERO
B Which should I choose, GlycINATOR or IgGZERO? Typically, GlycINATOR is the first choice since it displays enzymatic activity on all glycoforms present on IgG, including complex type, high mannose,…
/smartenzymes/antibody-deglycosylation/iggzero/PNGase F
best performance of the column is TBS buffer at pH 8.6. Other buffers with neutral pH containing 0.15 M NaCl can also be used but optimization may then be required….
/smartenzymes/glycan-profiling/pngase-f/Production of Fab Fragments
F(ab’)2 to Fab’ A beautiful way to circumvent the micro-heterogeneity and boost the low yield that is often the result of fragmentation using other proteolytic enzymes like pepsin or papain…
/smartenzymes/applications/preparative-applications/production-of-fab-fragments/