Monitoring mAb afucosylation using GlycINATOR
The level of core fucosylation on therapeutic antibodies have major impact on antibody dependent cell cytotoxicity. To study the core fucosylation, Suli Liu and Li Zang at Biogen have published a method using deglycosylation by GlycINATOR, reduction of the antibody and LC-MS analysis. Using this methodology the scientist could quantify the fucosylation within 1 h, including sample preparation and analysis. Liu and Zang argue that this methodology is particularly well suited for high-troughput analysis cell line development and process development of therapeutic mAbs.
