FabULOUS Fab Kit – Intact Fab fragments from mouse IgG
The FabULOUS (SpeB) enzyme digests IgG in the hinge region. The FabULOUS Fab kit is designed for digestion and purification of Fab fragments from mouse IgG antibodies. Incubation at optimized reducing conditions generates intact Fab, easily purified with spin columns.
- Digestion in the hinge with FabULOUS
- Affinity purification of Fab fragments using the light chain
- Preparation of Fab fragments from mouse IgG in less than 2 h